This method describes the extraction of quaternary amines, strongly basic amines and amines that are unstable at high pH levels, from biological fluids. The mechanism is mixedmode
hydrophobic and weak cation exchange, allowing acidic elution conditions.
Tags: Application Notes, Biological fluids, Clinical, Quaternary Amines
The first decision that needs to be made is what the final analysis will be for the analyte. This will have an impact on the sample and cartridge size, as well as the final elution solvent. Gas chromatography offers higher sensitivity than HPLC, while HPLC is better suited for ionisable species and very high molecular weights. If LC-MS is available, minimal sample clean-up may be required.
Tags: Manuals & User Guides
The ISOLUTE® HCX-Q SPE sorbent has been developed for the extraction of quaternary amines and polybasic
drugs from biological fluids, giving high reproducible recoveries and clean extracts.
Tags: Product Notes
The use of ISOLUTE HCX mixed-mode sorbents is widely accepted for providing high purity extracts of basic drugs from biological fluids. The ISOLUTE HCX-Q sorbent utilizes a combination of weak cation exchange and C8 non-polar retention mechanisms.
Tags: Product Notes
On July 1st, Biotage expanded its direct sales organization and opened the door to analytical customers in Italy. Account Manager Gianluca Pessoni is added to the Italian team, filling the ana...28 June 2017
This application note describes the operating conditions for the automated fractionation of EPH into aliphatic and PAH fractions using the ISOLUTE® EPH column in conjunction with the Biotage&re...20 February 2017
Now available, the Biotage 2016 catalog of analytical sample preparation products for chemistry professionals. This edition includes a comprehensive range of products for bioanalysis, forensic, cli...11 August 2015
Dilute and shoot (D&S) methods are promoted for use by analytical instrument vendors in clinical, forensic toxicology and bioanalytical laboratories. More sensitive LC-MS/MS systems make this s...27 March 2015
Now available, the Biotage 2015 catalog of analytical sample preparation products for chemistry professionals. This edition includes a comprehensive range of products for bioanalysis, forensic, cli...29 July 2014
What is the maximum capacity of an SPE column?
The capacity of a sorbent is defined as the total mass of strongly retained analyte that can be retained by a given mass of the sorbent under optimum conditions.
For most sorbents, this can range from 1-5% by weight. 100 mg of sorbent might retain 1-5 mg of analyte.
Ion exchange sorbents typically have a capacity of 0.3-1 meq/g.Browse more questions and answers >
|Part No.||Description||Product type||Pack Size||Price|
|986-0002-A||ISOLUTE® HCX-Q 25 mg/1 mL||Consumables||100||Log in for price|
||986-0005-A||ISOLUTE® HCX-Q 50 mg/1 mL||Consumables||100||Log in for price|
|986-0010-A||ISOLUTE® HCX-Q 100 mg/1 mL||Consumables||100||Log in for price|
||986-0025-P01||ISOLUTE®-96 HCX-Q 25 mg Fixed Well Plate||Consumables||1||Log in for price|
||986-0100-P01||ISOLUTE®-96 HCX-Q 100 mg Fixed Well Plate||Consumables||1||Log in for price|