The environmental persistence of pharmaceuticals is of increasing interest, with little known regarding removal during wastewater treatment. This study investigates the potential of commercially available QuEChERS kits for the extraction and clean-up of a suite of common over-the-counter (OTC) pharmaceuticals from water and sludge cake. Extracts are analysed using LC-MS/MS.
Tags: Acetaminophen, Analgesics, Application Notes, Cetrizine dihydrochloride, Diclofenac, Diphenhydramine hydrochloride, English, Environmental, LC-MS/MS, Over the counter pharmaceuticals, QuEChERs, Sludge cake, Water
Malachite green (MG) and crystal (gentian) violet (CV) are triphenylmethane dyes used in aquaculture as fungicides and ectoparasiticides. They are rapidly metabolised to the leucomalachite green (LMG) and leucocrystal (gentian) violet (LCV) metabolites that persist in fish tissue. MG and CV are banned in many countries due to their mutagenicity and carcinogenicity, but may still be used illegally. Monitoring methodologies must be able to detect the compounds at 1 ng/g (US) or 2 ng/g (EU). In order to meet these LODs for complex matrices such as oily fish, and the low UV absorbance of the leuco forms, LC-MS-MS is the analytical method of choice. This application note focuses on ease of use when compared to other methods, and describes a QuEChERS based approach incorporating the use of aluminium oxide in enhanced dispersive SPE to reduce the levels of fat in the final extract. Additional cleanup is provided by polymeric EVOLUTE SPE columns, and the resulting extract demonstrated low ion suppression. Analyte recoveries of 60-105%, with low rsds, are achieved. The method is also applicable to Brilliant green.
Malachite green, Leuco malachite green, Crystal violet, Leuco crystal violet, Brilliant Green, EVOLUTE, EVOLUTE CX, QuEChERS, LC/MS/MS, Salmon, Contamination, Gentian violet, SPE, Solid Phase Extraction, Polymer, Resin,
Tags: Application Notes, Brilliant Green, Column, Crystal Violet, Fish, Food, Food, Gentian Violet, LC-MS/MS, Leuco Crystal Violet, Leuco Malachite Green, Malachite Green, Salmon, Triphenylmethane Dyes, Veterinary drugs
The first decision that needs to be made is what the final analysis will be for the analyte. This will have an impact on the sample and cartridge size, as well as the final elution solvent. Gas chromatography offers higher sensitivity than HPLC, while HPLC is better suited for ionisable species and very high molecular weights. If LC-MS is available, minimal sample clean-up may be required.
Tags: Manuals & User Guides
15g QuEChERS AOAC 15mL Extraction Tube
Q0010 – 15V
Uses advised against:
Not for therapeutic or diagnostic use
Tags: English, Material Safety Data Sheets
QuEChERS EN 15 mL dispersive SPE
Tags: Material Safety Data Sheets
QuEChERS (pronounced “catchers”) stands for Quick, Easy, Cheap, Effective, Rugged, and Safe. The technique is designed to be simple and involve a minimum number of steps; but still allow for effective clean up of complex samples. The QuEChERS technique involves two stages.
Tags: Product Notes
EN 15662 General Fruit and Vegetables
Tags: Product Notes
The Biotage 2018 catalog of analytical sample preparation products for chemistry professionals is now available. This edition includes a comprehensive range of sample preparation consumables for bi...17 July 2017
On July 1st, Biotage expanded its direct sales organization and opened the door to analytical customers in Italy. Account Manager Gianluca Pessoni is added to the Italian team, filling the ana...28 June 2017
This application note from Biotage Labs describes a method for extracting nitrosamines from urine. The ISOLUTE® SLE+ sample preparation method allows for a fast extraction with sufficient sampl...15 June 2017
This application note describes the extraction of amphetamine and amphetamine-style compounds from hair matrix prior to GC/MS analysis following bead homogenization. This application note describe...20 March 2017
This application note describes the operating conditions for the automated fractionation of EPH into aliphatic and PAH fractions using the ISOLUTE® EPH column in conjunction with the Biotage&re...20 February 2017
This application note describes the extraction of 49 drugs of abuse from whole blood, prior to UPLC-MS/MS analysis. An effective and efficient ISOLUTE SLE+ protocol optimized the for ...05 January 2016
Now available, the Biotage 2016 catalog of analytical sample preparation products for chemistry professionals. This edition includes a comprehensive range of products for bioanalysis, forensic, cli...11 August 2015
Dilute and shoot (D&S) methods are promoted for use by analytical instrument vendors in clinical, forensic toxicology and bioanalytical laboratories. More sensitive LC-MS/MS systems make this s...27 March 2015
Biotage, pioneers in flash chromatography, started packing columns with silica for flash purification 30 years ago. Some of the first employees are still with us today. In this video, site director...08 January 2015
Now available, the Biotage 2015 catalog of analytical sample preparation products for chemistry professionals. This edition includes a comprehensive range of products for bioanalysis, forensic, cli...19 November 2014
This study suggests that a modified form of QuEChERS may be suitable for extraction and clean-up of pharmaceuticals in complex environmental matrices. Carried out by a collaborative ...29 July 2014
Buy your Biotage consumables through our website and shipping is free For a limited time, free shipping is applied to Biotage consumables orders through the Biotage website*. Let Biotage make orde...27 September 2010
Uppsala, Sweden, September 27, 2010 – Biotage® (STO: BIOT), a leading supplier of tools and technology for analytical and medicinal chemistry, announced its new line of Biotage/IST ISOLUT...
What is the maximum capacity of an SPE column?
The capacity of a sorbent is defined as the total mass of strongly retained analyte that can be retained by a given mass of the sorbent under optimum conditions.
For most sorbents, this can range from 1-5% by weight. 100 mg of sorbent might retain 1-5 mg of analyte.
Ion exchange sorbents typically have a capacity of 0.3-1 meq/g.Browse more questions and answers >
|Part No.||Description||Product type||Pack Size||Price|
|Q0000-50V||QuEChERS 50 mL Centrifuge Tube With Rack||Consumables||25||Log in for price|
|Q0010-15V||QuEChERS AOAC 15 g/15 mL Extraction Tube||Consumables||25||Log in for price|
|Q0020-15V||QuEChERS EN 10 g/15 mL Extraction Tube||Consumables||25||Log in for price|
||Q0030-15V||QuEChERS AOAC Fruit and Vegetables Clean up Tube||Consumables||25||Log in for price|
||Q0035-15V||QuEChERS EN Fruit and Vegetables Clean up Tube||Consumables||25||Log in for price|
||Q0050-15V||QuEChERS AOAC Waxed Fruit and Vegetables Clean up Tube||Consumables||25||Log in for price|
||Q0060-15V||QuEChERS EN Waxed Fruit and Vegetables Clean up Tube||Consumables||25||Log in for price|
|Q0070-15V||QuEChERS AOAC Pigmented Fruit and Vegetables Clean up Tube||Consumables||25||Log in for price|
||Q0080-15V||QuEChERS EN Pigmented Fruit and Vegetables Clean up Tube||Consumables||25||Log in for price|
|Q0090-15V||QuEChERS EN Highly Pigmented Fruit and Vegetables Clean up Tube||Consumables||25||Log in for price|