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SPE columns for fractionation of aliphatics and aromatics in soil

ISOLUTE EPH 1.45g/3ml
ISOLUTE EPH 1.45 g 3 mL ISOLUTE EPH 5 g 25 mL on VacMaster 20 ISOLUTE EPH 5 g 25 mL ISOLUTE EPH 5 g/25 mL

Reliably fractionate aliphatics from aromatics in soil extracts, for GC-FID or GC-MS analysis.


Part No. Description Product type Pack Size Price
928-0145-B ISOLUTE EPH 1.45 g/3 mL Consumables 50 Log in for price
928-0500-E ISOLUTE EPH 5 g/25 mL Consumables 20 Log in for price

ISOLUTE® EPH columns are designed to fractionate pentane or hexane based soil extracts into aliphatic (C8-C40) and aromatic (C10-C22) fractions, using a simple, easily automated procedure. The common problem of breakthrough of aromatic compounds into the aliphatic fraction is eliminated.

The approach is based on procedures published by MADEP (Massachusetts Dept of Environmental Protection) and the TPH Criteria Working Group.

Columns and optimised methodology for both manual and automated procedures are available.  The automated procedure provides reliable, high throughput fractionation with substantial redusctions in  solvent consumption and costs, and can eliminate the need to evaporate fractions prior to analysis.

ISOLUTE® EPH columns consist of a proprietary blend of silica and alumina that has been optimised to achieve clean, robust fractionation of the aliphatic/aromatic EPH components.  Fractionation efficency of the ISOLUTE EPH columns is shown in the chromatograms below.

Fractionation of EPH aliphatics and aromatics

Product purity, compatible with non-selective GC-FID analysis, is ensured by QC testing.  The absence of naphthalene in the aliphatic fraction is one of the QC parameters used to control the activity of ISOLUTE® EPH products.

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