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ISOLUTE® PLD+ Protein and Phospholipid Removal Plates

Effortlessly Enhance Analyte Sensitivity

Figure 1 PLD+ Product Note
PLD+ protein precipitation plate Figure 2 PLD+ FAQs Test 2 FAQ P1 Chromatogram Web isolute pld+ plate from below

ISOLUTE® PLD+ Protein and Phospholipid Removal Plates provide a very effective but extremely simple sample clean-up for LC-MS/MS analysis. Requiring next to no method development, ISOLUTE PLD+ can be integrated quickly and easily into routine workflow, increasing productivity and reducing instrument downtime. 


Part No. Description Product type Pack Size Price
121-5202 Collection Plate, 1 mL Square, Qty 50 Consumables 50 Log in for price
121-5203 Collection Plate, 2 mL Square, Qty 50 Consumables 50 Log in for price
121-5204 Piercable Sealing Cap Consumables 50 Log in for price
121-5213 Collection Plate, 2 mL Round, Qty 50 Consumables 1 Log in for price
918-0050-P01 ISOLUTE® PLD+ Protein and Phospholipid Removal Plate Consumables 1 Log in for price

Remove More Phospholipids - Get Better Sensitivity

ISOLUTE® PLD+ plates deliver cleaner extracts than other phospholipid removal products, meaning lower detection limits can be achieved. In the example below, signal-to-noise using ISOLUTE PLD+ was more than double when compared with competitor products (figure 1), leading to an immediate enhancement in sensitivity.


Figure 1.  Relative peak intensity, 20 pg/mL amitriptyline in plasma (Blue = ISOLUTE® PLD+, Orange = Competitor O, Red= Competitor P).

Excellent Protein and Phospholipid Removal

More than 99 % of phospholipids are removed as the sample passes through the multifunctional sorbent bed, leaving clean, purified extracts for analysis (figure 2). Removing phospholipids also improves analytical column lifetime, preventing the build-up of phospholipids over multiple injections, and maintaining analyte sensitivity over extended analytical runs.

Figure 2. Phospholipids in human plasma treated by a) protein precipitation and b) ISOLUTE® PLD+. Lysophospholipids and phospholipids present in plasma treated by protein precipitation (red and green respectively) are shown in 1b on an expanded scale for direct comparison with residual levels of lysophospholipids and phospholipids present in ISOLUTE® PLD+ treated plasma (purple and blue respectively).

Effective Sample Clean-up in 3 Simple Steps

Figure 3. Typical ISOLUTE PLD+ procedure

Using a simple solvent crash/filtration based procedure, proteins and phospholipids are simultaneously removed from plasma and other blood based samples while high, reproducible analyte recoveries are maintained. The optimised frit arrangement acts as a depth filter, efficiently trapping precipitated proteins, without blocking or plugging.

Trouble Free Processing

ISOLUTE® PLD+ plates have excellent flow characteristics, and do not plug or block during use. They can be processed easily using either positive pressure or vacuum based systems, and are compatible with most automated liquid handlers.

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