EVOLUTE® WCX Columns for Solid Phase Extraction of Quaternary Amines and Strongly Basic Compounds from Aqueous Samples

This technical note provides guidelines for the extraction of quaternary amines and strongly basic analytes from aqueous samples using EVOLUTE WCX (Method A). A method optimized for extraction of a wide range of basic compounds, allowing elution under mildly acidic conditions (Method B) is also detailed. This approach is recommended for the extraction of basic compounds when basic elution conditions are unsuitable, or acidic elution conditions are preferred due to LC-MS/MS compatibility. The procedures are described on page 1-2, with processing and optimisation guidelines on page 3. An example application showing the extraction of quaternary amines from urine and plasma illustrates the use of EVOLUTE WCX (Chlormequat, Neostigmine, Bretylium, Difenzoquat) for clean-up of biological fluid samples. BASIC, BIOTAGE, EVOLUTE, OASIS, SPE, POLYMER, SOLID PHASE EXTRACTION