Extraction of 8-oxodG from Biological Fluids Using ISOLUTE ENV+

This application note describes a SPE-UHPLC-MS/MS procedure for the analysis of 8-oxodG, a biomarker of oxidative stress, from various human biofluids using ISOLUTE ENV+ solid phase extraction columns. Oxidative stress is the imbalance between the production of reactive oxygen species (ROS) and the body's ability to eliminate them, in favour of the former. ROS have been linked with development of numerous diseases including Parkinson's and Alzheimer's and have also been associated with fertility problems. 8-Oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) is one of the mutagenic nucleobase modifications produced in DNA and the 2'deoxyribonucleotide pool, by the reaction of ROS. Measurement of 8-oxodG in biological fluids can provide a useful indicator of oxidative stress. 8-oxodG, sample prep, ENV+, urine, biological fluids, biomarker, oxidative stress, nucleotide,