Sample Preparation of a Drugs of Abuse Panel in Human Hair Using ISOLUTE® SLE+ Prior to UPLC-MS/MS Analysis

This application note describes a procedure for sample pre-treatment and extraction of a panel of 49 drugs of abuse from human hair, using Biotage® Lysera for matrix micropulverization, prior to clean up using ISOLUTE® SLE supported liquid extraction. The application note contains procedures optimized for 400 mL capacity column format and 200 mL and 400 mL supported liquid extraction plate formats, for higher throughput applications. The methodology delivers clean extracts and analyte recoveries mostly greater than 80% with RSDs lower than 10% for all analytes and LLOQ from 1 ng/mL.