Documents|Sample Preparation of a Drugs of Abuse Panel in Human Hair Using ISOLUTE® SLE+ Prior to UPLC-MS/MS Analysis
Sample Preparation of a Drugs of Abuse Panel in Human Hair Using ISOLUTE® SLE+ Prior to UPLC-MS/MS Analysis
This application note describes a procedure for sample
pre-treatment and extraction of a panel of 49 drugs of
abuse from human hair, using Biotage® Lysera for matrix
micropulverization, prior to clean up using ISOLUTE® SLE
supported liquid extraction. The application note contains
procedures optimized for 400 mL capacity column format and
200 mL and 400 mL supported liquid extraction plate formats,
for higher throughput applications. The methodology delivers
clean extracts and analyte recoveries mostly greater than
80% with RSDs lower than 10% for all analytes and LLOQ from
DownloadE-mail a CopySample Preparation of a Drugs of Abuse Panel in Human Hair Using ISOLUTE® SLE+ Prior to UPLC-MS/MS Analysis
Biotage is a Global Impact Tech Company committed to solving society’s problems. We offer workflow solutions and products to customers in drug discovery and development and diagnostics, analytical testing and water and environmental testing.
Biotage is contributing to sustainable science with the goal to make the world healthier, greener and cleaner – HumanKind Unlimited.
Our customers span a broad range of market segments including pharmaceutical, biotech, diagnostic, contract research and contract manufacturers as well as clinical, forensic and academic laboratories in addition to organizations focused on food safety, clean water and environmental sustainability.
Biotage is headquartered in Uppsala in Sweden and employs appr. 700 people worldwide. The Group had sales of 1,566 MSEK in 2022 and our products are sold in more than 80 countries. Biotage’s share (BIOT) is listed in the Large Cap segment on the NASDAQ Stockholm.