Part No: Issued year: 2011File size: 0.72mbFile type: pdf
Biotage AB was established in 1997 under the name Pyrosequencing AB. The Company made a number of acquisitions in the medicinal chemistry sector between 2003 and 2005. Following the disposal of the Biosystems business area, the Company consists of one business area – Discovery Chemistry. The Company’s head office is situated in Uppsala. Biotage applies the Swedish Code of Corporate Governance (“the Swedish Code”). The diagram below shows Biotage’s corporate governance model and how the central bodies interact.
Part No: Issued year: 2013File size: 0.11mbFile type: pdf
Biotage AB was established in 1997 under the name Pyrosequencing AB. The Company made a number of acquisitions in the medicinal chemistry sector between 2003 and 2005. Following the disposal of the Biosystems business area, the Company is mainly active within analytical and organic chemistry.
Part No: PPS445Issued year: 2017File size: 0.62mbFile type: pdf
Ease of use is what stands out as the top feature of Isolera™ flash chromatography
system for Professor Anna Bernardi, head of the synthetic organic chemistry
research group at the University of Milan. Her current research goal: developing
sugar-like molecules, called glycomimetics, for healthier life.
Part No: PPS443.V.1Issued year: 2019File size: 2.98mbFile type: pdf
Analysis of drug panels in urine samples can be challenging, and the trend towards larger panels including multiple drug classes compounds the issues faced during method development.
This white paper examines a number of aspects of sample preparation, and their impact on the success of subsequent LC-MS/MS analysis of broad urine panels.
Section 1 examines the applicability of various sample preparation techniques: supported liquid extraction, reverse phase SPE and mixed-mode SPE, to the various classes of drugs extracted. In addition, hydrolysis approaches: enzyme type and protocol used (time, temperature), are compared.
Mixed-mode reverse phase/cation exchange SPE is widely used for extraction of basic drug classes from urine, but the inclusion of drugs and metabolites that exhibit ‘non-typical’ functionality within urine panels can be problematic. Section 2 examines the impact of various parameters (interference wash strength, elution solvent composition) on analyte retention, elution and extract cleanliness with particular focus on zwitterionic (gabapentin, pregabalin) and non-ionic (carisoprodol, meprobamate) drugs.
Part No: Issued year: 2011File size: 0.07mbFile type: pdf
Through innovative patented technology based on molecularly imprinted polymers (MIPs)
you will experience improved clean up in your processes. With a high probability of success,
unwanted contaminants or high value desirables can be selectively extracted from your
processes, resulting in more efficient production and cleaner products.
Part No: P102Issued year: 2014File size: 0.6mbFile type: pdf
Designed polymers are a class of selective resins with engineered selectivities for particular target molecules or ‘classes’ of molecules. These designed polymers are obtained by careful tuning of their surface chemistry and morphology which allows them to exhibit unique separation capabilities. The tailored and optimized selectivity of designed polymers is utilized to conduct difficult separations that are not able to be accomplished with conventional separation resins or other techniques.
Part No: AN954Issued year: 2010File size: 0.09mbFile type: pdf
This paper demonstrates how the introduction of simple automated technology and a modification in analysis. Can
positively impact analytical results and overall throughput for critical environmental testing.
Part No: AN053-HORIssued year: 2015File size: 1.3mbFile type: pdf
Carbaryl, a commonly used insecticide for the past 20 years, has become a significant environmental concern throughout the world. Also known through its trademarked name Sevin, it is a cholinesterase inhibitor and can be toxic to humans, causing problems in the blood, nervous, and reproductive systems.
Part No: AN089-HORIssued year: 2014File size: 2.48mbFile type: pdf
Deoxynivalenol is a common mycotoxin found in agricultural grain crops and final consumer processed products. Most impacted are wheat, barley, and corn. Deoxynivalenol, also known as Vomitoxin, is produced by Fusarium fungi and has a unique dual-stage growth cycle of producing mold during warm daylight and toxin during cool nights. There is no known procedure or processing that will remove deoxynivalenol that is already present in grain.
Part No: AN001-HORIssued year: 2015File size: 1.35mbFile type: pdf
Hormones in drinking water are a growing concern, since the presence of these compounds can potentially be linked to serious health hazards such as human developmental and reproductive side-effects. Hormones are introduced into our environment in several ways. Natural and synthetic hormones that are given to livestock, pass though the animals, runoff into surface water, and leach into underground water supplies. Humans also produce and excrete natural hormone waste every day.
Part No: AN012-HORIssued year: 2010File size: 0.99mbFile type: pdf
The second Unregulated Contaminant Monitoring Regulation (UCMR2) program was developed to monitor US drinking water sources for currently unregulated compounds. EPA Method 529 is categorized under List 1, Assessment Monitoring in the UCMR2 program, and focuses on three explosives: 1,3-dinitrobenzene; 2,4,6- trinitrotoluene (TNT); and hexahydro-1,3,5-trinitro-1,3,5- triazine (RDX). The resulting data will be used by the EPA to determine whether or not regulatory standards should be established for these compounds.
Part No: AN057-HORIssued year: 2010File size: 0.72mbFile type: pdf
At present, pollution of freshwater algae has become aglobal environmental problem. Of all the different pollution types, microcystin LR is the most toxic and the most acute hazard as far as is known presently
Part No: AN078-HORIssued year: 2015File size: 1.25mbFile type: pdf
This application note was developed to demonstrate the extraction of five organophosphate compounds Monocrotophos, Diazinon, Malathion, EPN, and Methamidophos using one solid phase extraction method with one pre-treatment step of sodium chloride (NaCl). The method uses the Biotage® Horizon 4790 automated SPE extraction system. It will show the efficiency of the extraction while demonstrating excellent recoveries of OPP compounds using methylene chloride and minimal amounts of acetone after sample pre-treatment with sodium chloride. Methods were developed and results are shown using 47 mm Atlantic® HLB-H disks and carbon cartridges.
Part No: AN009-HORIssued year: 2015File size: 1.33mbFile type: pdf
Method 525.2 describes the procedure to determine a full suite of low concentration semi-volatile organic compounds in drinking water using solid phase extraction (SPE) or liquid–solid extraction (LSE) techniques. The City of Fort Worth, Water Department implemented an automated SPE process for the analysis of semi-volatiles by EPA Method 525.2 using the Atlantic® C18 solid phase extraction disk. Ethyl acetate, methanol and water were used to condition the Atlantic C18 disk prior to the extraction step. The extraction solvents used were a 1:1 mixture of methylene chloride and ethyl acetate. Extracts were then analyzed by GC/MS using a splitless injection technique.
Part No: AN108-HORIssued year: 2016File size: 2.35mbFile type: pdf
Drinking water is a critical resource important for human health. As water becomes more scarce, quality will become increasingly important and monitoring will be required more frequently and at lower concentration levels. The number of compounds and lower concentrations for monitoring will make extraction and analysis even more challenging.
Part No: AN019-HORIssued year: 2010File size: 0.92mbFile type: pdf
The City of Fort Worth, Water Department conducted an evaluation of the Atlantic™ solid phase extraction (SPE) C18 disks using EPA Method 608. This application note describes the procedure to determine low ppb levels of organochlorine pesticides and PCBs in wastewater. This procedure used methylene chloride as the primary extraction solvent, followed by a hexane solvent exchange. Extracts were then analyzed by GC/ECD using a pressure pulse injection technique.
Part No: AN021-HORIssued year: 2009File size: 0.89mbFile type: pdf
Method 1657 describes the procedure to determine low ppb levels of organophosphorus pesticides in municipal and industrial wastewater.
The City of Fort Worth Water Department implemented Automated SPE for the analysis of organophosphorous pesticides by EPA Method 1657, using the Atlantic™ C18 solid phase extraction disk.
The Biotage® Horizon 4790 Automated Extraction System with Envision®
Platform controller, and the DryVap® Automated Drying
and Concentration System were used in this application note.
Part No: AN072-HORIssued year: 2012File size: 0.88mbFile type: pdf
Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous environmental contaminants, naturally occurring in coal, crude oil, gasoline, and their byproducts (e.g. coal tar or creosote). In addition, PAHs are formed in the incomplete combustion processes of all organic materials, such as wood or fossil fuels. Consequently, the EU water framework directive (WFD) lists in its annex X the whole group of PAHs as priority hazardous substances.
Part No: AN038-HORIssued year: 2009File size: 2.13mbFile type: pdf
This application note demontrates that the Biotage® fully automated extraction, drying and concentration systems used with the Atlantic™ HLB disk are capable of extracting PAH compounds from sea water.
Typical extraction times using the Biotage® Horizon 4790 range from 20 to 25 minutes while drying and concentration on the DryVap® with the DryDisk® took approximately 35 minutes.
Part No: AN055-HORIssued year: 2015File size: 1.36mbFile type: pdf
Polybrominated diphenyl ethers (PBDEs) are a worldwide contamination problem. Structurally similar to PCBs, these compounds are long-lived in the environment and can bio-accumulate throughout the food chain. The health hazards of these chemicals have attracted increasing scrutiny and, as such, a great deal of research and regulations have been implemented to manage and control them.
Part No: AN011-HORIssued year: 2010File size: 0.93mbFile type: pdf
The second unregulated contaminant monitoring regulation (UCMR2) program was developed to monitor US drinking water sources for currently unregulated compounds. EPA Method 527 is categorized under List 1; Assessment Monitoring in the UCMR2 program. It focuses on a wide range of semi volatile organic contaminants including pesticides that were deferred during the first UCMR, flame retardants, and pyrethroid pesticides. This application for EPA Method 527 employs SPE with analysis by gas chromatography / mass spectrometry (GC/MS).
Part No: AN035-HORIssued year: 2009File size: 0.83mbFile type: pdf
The purpose of this application note is to demonstrate the use of a fully automated solid phase extraction (SPE) and concentration system that provides fast extraction while improving the quality and consistency of results for EPA Method 8270D.
Part No: AN104-HORIssued year: 2016File size: 1.48mbFile type: pdf
Drinking water is an important resource that can provide significant exposure to humans if it is polluted. Analysis of source water, which may come from surface water, groundwater or treated water must be evaluated for possible contaminants to ensure regulatory compliance.1 Drinking water at the tap is often evaluated for contaminants that may be introduced through leaks in the pipes or pipe materials.
Part No: AN036-HORIssued year: 2009File size: 0.86mbFile type: pdf
The purpose of this application note is to demonstrate the capabilities of the Biotage® Horizon 4790 Automated Extractor System when used for the analysis of nitramine, nitroaromatic, and nitrate ester compounds in surface and ground water.
Part No: AN058-HORIssued year: 2010File size: 0.9mbFile type: pdf
The purpose of this application note is to demonstrate the capabilities of the automated Horizon Technology solution when used for the analysis of EPA method 8061A phthalate ester compounds in surface and ground water. Method 8061A states gas chromatography/mass spectrometry (GC/MS) can be used as an alternative for compound confirmation for phthalate esters.
Part No: AN064-HORIssued year: 2015File size: 1.69mbFile type: pdf
Although not frequently a problem, recent incidents at sea have led to large amounts of crude oil being released and dispersed throughout the Gulf of Mexico, such as in the Deepwater Horizon Gulf oil spill of 2010. Originally presumed to be Louisiana Sweet Crude, sample testing later revealed a harsher form of crude containing a high amount of asphaltenes was actually being released.
Part No: AN063-HORIssued year: 2012File size: 0.9mbFile type: pdf
Oftentimes it becomes necessary to test sea water for oil and grease analytes. Whether it is due to a controlled release of effluent water from a waste water treatment plant (usually during large rain storms) or a large oil spill, there is a need generated for a reliable, automated extraction and evaporation system.
Part No: AN061-HORIssued year: 2010File size: 0.74mbFile type: pdf
Throughout the world, a lack of regulation has been blamed for the detection of pharmaceuticals and personal care products (PPCP) in some bodies of water. While the detected amounts are usually relatively small, the persistence of these compounds means that they can cause untold damage to the ecosystem for years to come.
Part No: AN065-HORIssued year: 2010File size: 2.07mbFile type: pdf
Although not frequently a problem, recent incidents at sea have led to large amounts of crude oil being released and dispersed throughout the Gulf of Mexico. Originally presumed to be Louisiana Sweet Crude, sample testing later revealed a harsher form of crude containing a high amount of asphaltenes was actually being released. The differences are substantial, as Louisiana Sweet degrades more readily in nature than crude oil containing asphaltenes.
Part No: Issued year: 2014File size: 0.12mbFile type: pdf
This poster describes the development and validation of a method for supported liquid extraction of serum cortisol, with analysis by UPLC-MS/MS. The aim of this study was to develop a candidate reference method that could then be used to underpin the UK NEQAS Cortisol scheme.
MSACL EU 2014
Part No: AN004-HORIssued year: 2009File size: 0.72mbFile type: pdf
This evaluation study was conducted to determine the feasibility of the Biotage SPE-DEXÒ 4790 Automated Extraction System for the extraction of herbicides and pesticides. The preliminary results demonstrate the capability of this method for the automated SPE extraction of organic compounds.
Part No: Issued year: 2017File size: 0.27mbFile type: pdf
This poster describes a simple solid phase extraction method using EVOLUTE® EXPRESS ABN columns for the extraction of the marine toxins okadaic acid (OA), dinophysistoxins (DTX1 and DTX2), ciguatoxin 3C (CTX3C) and tetrodotoxin (TTX).
Part No: AN057Issued year: 2012File size: 0.21mbFile type: pdf
The formation of diarylethers by reacting an arylhalide and phenol is usually a reaction demanding long reaction times, high temperatures and strong bases, in order to obtain acceptable yield. The substitution patent of the electrophile and the nucleophile affects the reaction times mostly. A sterically hindered electrophile and a strongly deactivated nucleophile as outlined in the (Scheme 1) below, gives a very low yield (13 %) at conventional reflux for 2 weeks.1,2 Remainder was
recovered starting material. We have previously reported the dramatically shortened reaction time to 1 hour along with improved yield running the reaction outlined in the Scheme by heating by microwaves.
Part No: P131Issued year: 2015File size: 0.47mbFile type: pdf
DMSO and DMF are suitable injection solvents for reversed-phase flash purification. DMSO shows it can be loaded in larger volumes (up to 0.05 mL/g of C18 media or 3.5% of a column volume) without affecting chromatographic separations or carrying compounds with it.
Part No: AN101-HORIssued year: 2016File size: 1.07mbFile type: pdf
This application note highlights a SPE disk which was specifically designed to increase the recoveries of traditionally difficult polar compounds as well as help to increase the diversity of the traditional 525.2 list to include those compounds which are of concern in today’s society.
Part No: P171Issued year: 2017File size: 0.69mbFile type: pdf
This poster demonstrates protocols for the determination of a range of drugs of abuse following collection with the NeoSal™ oral fluid device and GC/MS analysis. The drug suites includes amphetamines and synthetic cathinones, barbiturates, benzodiazepines, cocaine, opiates, cannabinoids and synthetic cannabinoids.
SOFT 2017, Boca Raton