Part No: TN118Issued year: 2006File size: 0.07mbFile type: pdf
ISOLUTE HM-N is a modified form of diatomaceous earth that can efficiently absorb aqueous samples. ISOLUTE HM_N is chemically inert and stable in the pH range 1-13. These characteristics make it a versabile material that plays an important role in many sample preparation applications.
Part No: Issued year: 2006File size: 0.83mbFile type: pdf
• Novo Nordisk A/S & radiochemistry at Novo Nordisk A/S
• Introduction to radiochemistry in general
• Radiochemistry & microwaves
• Selected case stories from the labs at Novo Nordisk A/S
• Applications of radiolabelled compounds
• Summary and acknowledgements
Part No: TechTipIssued year: 2011File size: 0.09mbFile type: pdf
This month’s topic of discussion will be the operation of the valves within the SPE-DEX® 1000/3000 extractors. There are several internal valves in the extractors, the solvent to waste valve, the sample collect valve and the water to waste valve.
Part No: PPS584Issued year: 2019File size: 0.46mbFile type: pdf
Dr. Picado works within the Structural Genomics Consortium
(SGC), housed under the Eshelman School of Pharmacy at the
University of North Carolina. Their team consists of chemists
and biologists who all work under the SGC. We were able
to discuss Dr. Picado’s most recent project at SGC and why
Biotage’s columns made such an impact.
Part No: P016Issued year: 2007File size: 0.16mbFile type: pdf
In order to obtain a fast synthesis of an N-unprotected tripeptide, microwave (mw) assisted deprotection, coupling and partial deprotection were applied starting with a purified protected dipeptide [Fig. 1]. The three consecutive mw assisted steps were performed without any intermediate purifications and were accomplished within some 30 minutes. The resulting crude mixture containing the Boc deprotected tripeptide ester was shown to be quite complex [Fig. 2]. The crude product was subjected to automated reversed phase flash chromatography, followed by automated fraction pooling and “thin film evaporation”. The total time for the entire procedure was 1 h 20 min. The purity of the product was > 99%.
Part No: P197Issued year: 2019File size: 1.01mbFile type: pdf
Perfluorinated compounds (PFCs) are a group of compounds that have been used in a wide array of industrial and household applications, including fabric protectors, non-stick coatings for cookware, coatings for food packaging and in some fire-fighting foams.
Their persistence in the environment allows them to accumulate in water sources, particularly those used for consumption.
This work will demonstrate the use of a cartridge-based extraction
system to extract and quantify up to 24 PFC compounds in drinking
water to highlight the challenges associated with this application.
Part No: PPS484Issued year: 2018File size: 0.21mbFile type: pdf
Dr. Simon Hammann is Postdoctoral Researcher at the
Department of Anthropology and Archaeology, University
of Bristol. By combining chemistry with archeological
research, he is able to look back in time and has produced
the first direct chemical evidence for dietary cereal
processing among Romans.
Part No: PPS433Issued year: 2016File size: 0.78mbFile type: pdf
Customer Case: Japan Frozen Foods Inspection Corporation.
The Japan Frozen Foods Inspection Corporation (JFFIC) made the transition from traditional mouse
testing of shellfish toxins, to instrumental analysis by developing original methods. In the sample
preparation procedure JFFIC uses EVOLUTE® ABN hydrophilic/hydrophobic SPE columns from Biotage.
Part No: PPS426Issued year: 2016File size: 0.38mbFile type: pdf
Customer case: Welsh Water. Analytical on-line cartridges for environmental testing from Biotage were released in 2016. Russell Gibbs from Dŵr Cymru Welsh Water has just started using them for drinking water testing.
Part No: CM-TRIT-0810Issued year: 2010File size: 0.26mbFile type: pdf
Trityl-ChemMatrix is mainly used to obtain a protected peptide as is has very low TFA cleavage conditions (under 1%). This resin therefore has the same cleavage conditions as the Cl-Trityl-polystyrene. On request, Trityl-ChemMatrix resins can
be offered preloaded will all 20 natural amino acids. Other amino acids can be preloaded on special request. Please take note that the Cl-Trityl-ChemMatrix resin is not offered for stability reasons. It is most effective to start with the Trityl-
OH-ChemMatrix resinchlorinate the resin and then add the amino acid of desire.
Part No: PPS448.v2Issued year: 2017File size: 0.53mbFile type: pdf
The TurboVap® 96 is a high speed concentrator designed to work with 96-well microplates and deep-well plates. It is an efficient alternative to the constant monitoring and long evaporation times that are characteristic of conventional techniques — with the added bonus of unattended operation.
Part No: TV-SS-07Issued year: 2010File size: 0.23mbFile type: pdf
In order to decide which model TurboVap will best fit a laboratory’s application, the following questions should be asked, along with following the flowchart on the reverse side:
• After extraction on the Dionex ASE, what is the matrix of my extract?
• After extraction on the Dionex ASE, will the extract require further cleanups or drying steps?
• What other types of samples may require concentration that are not extracted on the ASE, so that I may select the model TurboVap that will be suitable for all types?
• Do I have enough Dionex ASE extracts and enough non-ASE extracts to justify two separate model TurboVaps?
• Does it make sense for me to purchase a standard TurboVap model now, and convert with the ASE compatible kit later?
Part No: TV-SS-05Issued year: 2010File size: 0.24mbFile type: pdf
In order to concentrate to 1.0 mL of solvent, the TurboVap must be configured such that the sensor is mounted with spacers on the bottom plate of the internal rack (Figure 1). To concentrate to 0.5 mL of solvent, the TurboVap must be configured such that the sensor is flush to the bottom plate of the internal rack (Figure 3). If the TurboVap is not correctly configured, the light beam from the sensor will not be aligned with the desired endpoint of the tube.
Part No: UI324Issued year: 2015File size: 0.75mbFile type: pdf
In determining the gas flow rate, the goal is to achieve
the maximum evaporation rate possible without
splashing or compromising the sample. During
evaporation the distance between the nozzle tips
and the liquid surface increases, therefore, the flow
rate may be safely increased as the evaporation
progresses to shorten evaporation time, if so desired.
Part No: PPS438.V.12Issued year: 2019File size: 1.67mbFile type: pdf
TurboVap® is a second generation product which builds on the solid foundations of the historic TurboVap product line and incorporates many new customer-driven features. It still features the highly efficient patented gas vortex shearing technology, which is synonymous with the TurboVap brand.
Part No: AN017-HORIssued year: 2009File size: 1.36mbFile type: pdf
The purpose of this application note is to demonstrate the use of a fully automated solid phase extraction (SPE) and concentration system that provides fast sample preparation while improving the quality and consistency of results for the Total Petroleum Hydrocarbon (TPH) compounds from C10 to C40 as dictated by EPA Method 8015.
Part No: P185.V.1Issued year: 2018File size: 0.86mbFile type: pdf
US EPA method 625 is used to determine acidic, basic, and neutral semi-volatile organic compounds (SVOC) in municipal and industrial wastewater. Revision A of this method is applied to a total possible list of 364 compounds that include; polynuclear aromatic hydrocarbons, chlorinated hydrocarbons, pesticides, phthalate esters, organophosphate esters, nitrosamines, haloethers, aldehydes, ethers, ketones, anilines, pyridines, quinolones, aromatic nitro compounds, and phenols.
Part No: AN857Issued year: 2016File size: 2.29mbFile type: pdf
This application note describes a protocol for the extraction of 1,25 di-OH Vitamin D2 and 1,25 di-OH Vitamin D3 metabolites from serum using supported liquid extraction prior to LC-MS/MS detection.
A calibration range between 5 and 500 pg/mL is demonstrated using a starting volume of 0.25 mL of serum. Sensitivity is maximized through the use of a simple PTAD derivatization and formation of a methylamine complex.
The method can be easily automated using the Biotage Extrahera. Details of the automated procedure and data comparing manual and automated method performance are included.
Part No: Issued year: 2011File size: 0.16mbFile type: pdf
Molecularly Imprinted Polymers (MIPs) are highly cross-linked polymers with selective binding sites engineered to contain recognition elements in defined positions1. MIPs have been developed for a large variety of small molecules, peptides, carbohydrates and even proteins. The schematic 0of a MIP site.
Part No: P055Issued year: 2013File size: 0.7mbFile type: pdf
Free urinary cortisol levels can be an indicator of a variety of disorders, such as Cushing Syndrome. This poster presents a simple method for the extraction of cortisol from urine demonstrating good recoveries and low ion suppression. LC-MS/MS analysis was performed using a Waters 2795 Liquid Handling Unit coupled to a Quattro Ultima Pt triple quadrupole MS using electrospray ionization, operated in the MRM mode. Supported liquid extraction method development resulted in recoveries greater than 90% for cortisol spiked urine. Calibration curves constructed using this method from 25-2000 ng/mL, demonstrated excellent linearity with coefficients of determination greater than 0.99.
Cortisol, Stress, Cushin's Syndrome, SLE, Supported Liquid Extraction, SPE, LLEMSACL, San Diego, 2013
Part No: Issued year: 2004File size: 0.39mbFile type: pdf
•Metal-mediated [2 + 3] cycloaddition to coordinated nitriles
-- synthesis of new transition metal complexes
-- synthesis of new heterocycles
-- enantioselective synthesis of heterocycles
role of microwaves?
• Platinum-catalysed cycloisomerisation of alkynes
-- Synthesis of Taxol-like compounds
-- highly efficient and atom economic chemistry
role of microwaves?
Part No: AN061Issued year: 2012File size: 0.63mbFile type: pdf
Acetone is successfully used as solvent in normal-phase flash chromatography when used with an Isolera™ Spektra flash purification system. The new λ-All detection and baseline correction features provide compound detection at all wavelengths in the detector’s range while minimizing any baseline drift due to solvent UV absorption.
Part No: P186Issued year: 2018File size: 0.2mbFile type: pdf
With the growing popularity and use of mass-directed flash
chromatography to purify specific compounds, it is important to understand that the sample’s compounds may not be detected as expected (e.g. M+H or M-H ions) because they may fragment or form adducts in the mass detector. This is true with both synthetic and natural product research.
Part No: PPS410Issued year: 2015File size: 0.7mbFile type: pdf
User Case: Professor Roger Strömberg, Karolinska Institutet. One of Professor Strömberg’s projects at Karolinska Institutet
involves the synthesis of artificial nucleases and PNAzymes. In
2013, his research group acquired a Biotage® Initiator+ Alstra™
peptide synthesizer for making PNAs and peptides.
Part No: PPS409Issued year: 2015File size: 1.61mbFile type: pdf
RIKEN is Japan’s largest research organization with 3,400
researchers from more than 50 countries. The chemists in RIKEN’s
Sugiyama Laboratory use EVOLUTE® EXPRESS solid-phase extraction
plates from Biotage in the analysis of clinical trial samples
for pharmacokinetic studies.
Part No: PPS432Issued year: 2016File size: 0.4mbFile type: pdf
Customer Case: Hitachi, Ltd. Healthcare Innovation Center. Hitachi’s R&D group has developed a new method for analyzing pharmacodynamics in cultured cells, in a study aimed at improving the pass rate in clinical trials. Mr. Ryosuke Takahashi tells the story.
Part No: Issued year: 2013File size: 0.43mbFile type: pdf
User report: ISOLUTE SLE+. Shin Nippon Biomedical Laboratories, Ltd. use ISOLUTE® SLE+ to prepare biological samples for pharmacokinetic testing as part of a drug discovery and development program. We had the chance to speak with Mr. Mitsuhiko Kawabata and Ms. Akiko Toda of the pharmacokinetics group.
Part No: T82Issued year: 2009File size: 0.16mbFile type: pdf
Oil & Grease standards come in pre-measured Teflon tubes of 10 mL each. Each tube contains 20 mg of n-Hexadecane and Stearic Acid for a total concentration of 40 mg. Care must be taken upon the introduction of the spiking solution to the sample bottle.
Part No: PPS406Issued year: 2015File size: 2.25mbFile type: pdf
Tokyo based PeptiDream Inc. is a company specializing in non-standard peptide therapeutics containing non-standard amino acids, and conducts research and development of new drug candidates. PeptiDream uses the peptide synthesizers Syro I and Biotage® Initiator+ Alstra™ for the synthesis of various kinds of peptides to support drug discovery and development.
Part No: AN049-HORIssued year: 2009File size: 0.74mbFile type: pdf
The DryVap® Concentrator System was intentionally designed to provide enhanced recoveries of semivolatile organic compounds, such as those found in EPA Method 8270. Its unique design of using an internal immersion heater vs. an external water bath, vacuum, and a sparge gas which is directed onto the surface of the solvent during the final stages of the evaporation process, allows the DryVap® System to achieve very good recoveries for a variety of tests.
Part No: AN042Issued year: 2003File size: 0.08mbFile type: pdf
Thin-layer chromatography (TLC) is a commonly used method development tool for flash purification. Product mixtures separated using TLC generally can be purified by flash chromatography using the identical solvent system. The method transfer accuracy of TLC to flash is based on the TLC silica and flash silica properties. When identical, the equation 1/Rf = CV holds, where Rf is the retention factor of a compound separated by TLC, and CV is the number of column volumes required to elute a compound. Cartridge loading capacity is based on the difference in CV (DCV) between two adjacent compounds.
Part No: Issued year: File size: 0.62mbFile type: pdf
The utilization of bound reagents for the solution phase synthesis has become an important tool in synthesis of biologically active molecules. The disadvantage of this technique is the relative slow rate of reaction. Microwave irradiation has been used to overcome this problem and increase rate of reaction of solid-assisted solution phase synthesis. This presentation covers developing efficient and robust strategies for the preparation of an array of cyclic tertiary amines using microwave irradiation in conjunction with bound reagents and scavengers such as MP-cyanoborohydride.
Part No: Issued year: 2015File size: 0.94mbFile type: pdf
The crossreactivity of molecularly imprinted polymers (MIPs) and its
practical implications are discussed. Screening of MIP libraries is presented as a
fasttrack route to discovery of resins selective towards new targets, exploiting the
fact that MIPs imprinted with one type of template molecule also show recognition
to related and sometimes also to apparently unrelated molecules. Several examples
from our own and others’ studies are presented that illustrate this crossreactivity and
the pattern of recognition is discussed for selected examples.
Part No: PPS482Issued year: 2018File size: 1.01mbFile type: pdf
We combine a robust, reliable UV (Visible/Ultraviolet
absorbance detector) monitor with a specialized
wireless tablet optimized for use in sensitive*
environments. The Biotage UV Monitor kit features
the latest in fiber optic technology and a powerful
SW package to provide the most effective and
flexible UV monitoring system for Biotage® Flash 150
and Biotage® Flash 400 purification systems.
Part No: AN117-HORIssued year: 2017File size: 4.2mbFile type: pdf
The US EPA monitors a variety of chemicals in water that may cause harm to humans or wildlife to minimize exposure. Method 625 was developed by the Office of Science and Technology in the Clean Water program to monitor a large suite of semivolatile chemicals in wastewater for compliance with the National Pollution Discharge Elimination System (NPDES).
Part No: PPS525Issued year: 2017File size: 0.19mbFile type: pdf
Safety is always a prime consideration in a laboratory. While working with organic solvents and samples that may contain harmful analytes, it makes sense to shield the operator from hazardous conditions whenever possible.
Part No: PPS468Issued year: 2017File size: 0.71mbFile type: pdf
Producing peptides for research is challenging. Biotage has developed
a holistic approach to the entire peptide workflow via an automated solution, designed for dedicated peptide researchers and those new to the field.
Part No: PPS466.GermanIssued year: 2017File size: 0.41mbFile type: pdf
Modelle haben gezeigt, dass sich die zur Herstellung
eines Zielmoleküls benötigte Zeit mit dem automatisierten
Workflow von Biotage um bis zu achtzig
Prozent verkürzen lässt, sodass auch die Projektlaufzeiten
entsprechend verkürzt werden können.
Part No: PPS463Issued year: 2017File size: 1.74mbFile type: pdf
Traditional approaches to compound purification involving chromatography utilize large volumes of relatively toxic and expensive solvents, and significantly contribute to the environmental footprint of organizations involved in molecular research. Current directives for greener chemistry have put pressure on organizations to reduce the
environmental impact of their work.
Part No: PPS463.gerIssued year: 2018File size: 0.68mbFile type: pdf
Bei herkömmlichen Aufreinigungsverfahren mittels Chromatographie kommen große Mengen verhältnismäßig giftiger und teurer Lösungsmittel zum Einsatz, die sich negativ auf den ökologischen Fußabdruck von Unternehmen in der Molekularforschung auswirken. Neue Richtlinien für einen verantwortungsvolleren Umgang mit Chemikalien zwingen Unternehmen, die Umweltauswirkungen ihrer Arbeit zu reduzieren. Durch Einblicke in die Arbeitsabläufe dieser Unternehmen und neue Entwicklungen im Bereich Flash-Chromatographiesysteme und -säulen lassen sich Verbrauch und Toxizität der für die Aufreinigung eingesetzten Lösungsmittel und somit auch die Umweltbelastungen dieser Verfahren reduzieren.
Part No: PPS466Issued year: 2017File size: 1.4mbFile type: pdf
Using the Biotage automated workflow, models have shown that the time taken to produce a target molecule can be reduced by up to eighty percent, allowing project delivery timescales to be reduced
Part No: PPS356Issued year: 2014File size: 1.19mbFile type: pdf
To save money on consumables, many chemists choose to reuse
silica flash cartridges. This is true but risks purification results because chromatographic separation performance will change from run to run which reduces purification quality, especially in normal phase systems.
Regardless of the cartridge brand used, repeated use of silica flash cartridges results in loss of compound resolution and therefore fraction purity.
Part No: TechTipIssued year: 2011File size: 0.07mbFile type: pdf
It’s important to replace these filters to ensure proper operation and protection of the internal solvent delivery valves. This filter not only traps particulates, but also protects the valves as particulates may get trapped within and could lead to improper solvent dispensing even when the extractors are at idle.
Part No: TN121Issued year: 2006File size: 0.07mbFile type: pdf
In medicinal chemistry reactions are often carried out in solvents such as DMSO. Once the reaction is complete, it is usually necessary to separate the products of the reaction from reagents and by-products. This can be performed using strong cation exchange sorbents (e.g. ISOLUTE SCX-2 or ISOLUTE Flash SCX-2) to selectively isolate the basic compounds from the reaction mixture. (see Figure 1 for structure of ISOLUTE SCX-2 and ISOLUTE Flash SCX-2 sorbents)
Part No: TN135.06Issued year: 2006File size: 0.09mbFile type: pdf
One of the most common work-up techniques in medicinal chemistry is liquid-liquid extraction (LLE) using a
separating funnel. The technique is used for removal of water soluble impurities, solvent exchange, water
removal or work-up of aqueous reaction mixtures.
Part No: TN135Issued year: 2006File size: 0.1mbFile type: pdf
One of the most common work-up techniques in medicinal chemistry is liquid-liquid extraction (LLE). The technique is used for removal of water soluble impurities, solvent exchange, water removal or work-up of aqueous reaction mixtures.
Part No: PPS352.v3Issued year: 2015File size: 7.34mbFile type: pdf
Biotage offers a range of products for reaction mixture work-up and organic sample preparation. The solutions include a range of techniques and also address throughput requirements from filtration, catch & release, and scavenging cartridges in organic synthesis workflows.