Part No: Issued year: 2014File size: 1.02mbFile type: pdf
Testing for drugs of abuse in oral fluids can strongly benefit the criminal justice field as a less invasive and cost-effective approach for drug detection when compared to blood or urine sampling. Oral fluid analysis has facilitated laboratory analysis for many drugs of abuse and is a constantly evolving analysis procedure which benefits from increasingly sensitive methods of detection. The Laser Diode Thermal Desorption (LDTD) source combined with Mass spectrometry is presented as a new screening tool for drug analysis in Oral Fluids. Analysis speed of LDTD provides accurate results in seconds in combination with exceptional specificity of MS instruments make a powerful platform for the screening of different drugs of abuse and new emerging drugs. Different extraction procedures are available; however those methods depend on specific drug conditions: basic or acid drugs / hydrophilic or hydrophobic. A new extraction approach, Supported Liquid Extraction (SLE+) is evaluated as generic extraction procedure.
Part No: UI400Issued year: 2018File size: 0.11mbFile type: pdf
The tasks carried out during the Preventive Maintenance (PM)
visit shall include servicing the listed components and a final
check, which is based on factory acceptance and qualification
tests. These two procedures constitute a Gilson Preventive
Part No: AN056Issued year: 2012File size: 0.2mbFile type: pdf
Quinolines are an important class of broad-spectrum antibiotics1 that were traditionally obtained by refluxing an aniline and diethyl ethoxymethylemalonate (Scheme 1) for several hours often in low yield.2 Heating the reaction mixture by microwaves to temperatures above the boiling points, with or without solvents, improves the yields and shortens the reaction time dramatically.3-6
Part No: AN106Issued year: 2015File size: 0.3mbFile type: pdf
The term “Green Chemistry” has become a major part of the
science community’s lexicon. In this application note we will
look at two areas for flash chromatography:
1. Replacing chlorinated solvents with those considered more
2. Reducing solvent use and waste generation with more
thoughtfully applied chromatography principles.
Part No: P158Issued year: 2017File size: 0.27mbFile type: pdf
As reversed-phase flash chromatography gains traction in
medicinal chemistry labs the need to monitor its cost and
safety are becoming more important. Commonly used
reversed-phase solvents typically include water with an
organic solvent such as methanol or acetonitrile – each
have advantages and disadvantages.
Part No: AN801Issued year: 2003File size: 0.27mbFile type: pdf
The heat flow parameter can be used to monitor the progress of a reaction, and can be useful
in determining reaction rates. A heat flow probe study was executed on the Advantage
Series™ 3400 process chemistry workstation for the hydrolysis of acetic anhydride in water
at temperatures from 25 to 55 °C. The observed rate constant for the hydrolysis was determined
from the heat flow measurements. An Eyring plot was produced and the enthalpy and
entropy of activation were determined. Finally, the heat of reaction was measured based on
the total amount of heat that was observed during the hydrolysis.
Part No: AN036Issued year: 2001File size: 0.55mbFile type: pdf
Activated carbon is an adsorbent media used to remove colored compounds from solution. Using this media in the Biotage FLASH-AC cartridge as a packed bed improves adsorbent performance. Increasing the temperature of the solvent modifies adsorption and alters performance of the decolorization process. Elevated temperatures increase product solubility, therefore requiring less solvent than at room temperature. The increased concentration of product in a hot solution also simplifies crystallization.
Part No: AN040Issued year: 2003File size: 0.4mbFile type: pdf
Research in CNS drugs is primarily centered on nitrogen heterocycle chemistry. Basic amines are difficult to purify using traditional silica chromatography because of strong interactions between acidic silica and the molecules’ basic amine groups. These interactions cause band spreading and poor compound recovery. Solutions employed to counteract this phenomena include adding a competing amine (e.g. triethyl amine or ammonium hydroxide) to the flash chromatography solvent system or using reversed-phase HPLC with a buffered solvent system.
Part No: AN118-HORIssued year: 2017File size: 1.2mbFile type: pdf
Hexane extractable material (HEM), also known as Oil & Grease extraction is a simple water quality measurement. The measurement can be used to assess pollution for compliance or as a measure of influent contamination to protect a waste treatment plant from levels that might cause malfunction.
Part No: P172Issued year: 2017File size: 0.49mbFile type: pdf
We investigate the use of reversed-phase high performance flash chromatography (HPFC) to quickly purify large quantities of crude synthetic peptides.
(6th Solid Phase Peptide Synthesis Symposium & 12th Australian Peptide Conference, Queensland, Australia, Oct. 2017)
Part No: Issued year: 2018File size: 0.66mbFile type: pdf
This poster presents a high sensitivity method for extraction and analysis of estrogens in human plasma. Sample preparation utilizes Biotage ISOLUTE SLE+ plates, providing high analyte recovery and low matrix effects.
MSACL 2018, Palm Spings, CA
Part No: AN041-HORIssued year: 2010File size: 0.83mbFile type: pdf
This application note investigates the applicability of automated solid phase extraction using the Biotage(r) Horizon 4790 extractor and DryDisk solvent drying system to extraction of a wide range of different compounds from phenols, to triazines, to organochlorine and organophosphorous pesticides spiked into tap water at a concentration of 1 ug/L.
Part No: Issued year: 2014File size: 0.34mbFile type: pdf
In this poster, an IONICS 3Q 220 triple quadrupole mass spectrometer using a load, wash, elute method protocol with recently advanced solid phase extraction (SPE) sorbent technology (EVOLUTE EXPRESS WCX) was used to perform analysis of the two compounds. As a result low levels of metanephrine and normetanephrine are detectable in plasma with short sample preparation and LC run times. This LC-MS/MS method provides a fast, sensitive, accurate, and reproducible solution for the analysis of ME and NME in plasma.
Part No: AN303Issued year: 2007File size: 0.14mbFile type: pdf
Heck reactions utilizing a thermostable catalyst in combination with microwave irradiation have been performed. A substantial enhancement of reaction rates as well as excellent regiocontrol could be obtained under microwave conditions without using inert gas. A slight increase in yield was also noted.
Part No: CM-HMPB-0810Issued year: 2010File size: 0.29mbFile type: pdf
For peptides acid, we recommend using the HMPB-ChemMatrix
as this resin will provide high crude purity and a recovery yield of
90-95%. The Wang-ChemMatrix will produce similar crude
peptide purity, but the recovery yield is lower (60-70%). On
request, we can provide preloaded HMPB-ChemMatrix resins with
all 20 naturally occurring amino acids. Other amino acids can be
preloaded on special request.
Part No: PPS535Issued year: 2017File size: 0.8mbFile type: pdf
When a new piece of equipment is introduced the question is always raised about performance. Does it perform as well as its predecessor? What about the performance over time? This technical note will provide data to describe the performance of the SPE-DEX 5000, providing solid phase disk extraction (SPE), compared to standards required by environmental methods.
Part No: UI402Issued year: 2019File size: 0.36mbFile type: pdf
Confidence is what you need before you separate your precious
compound on a new brand of flash chromatography column.
Predictably getting your compound in a pure state is what you
want, and if you can get it faster and use less solvent all the
better. After all, your project needs to be completed on time,
and trying something new is not always appealing.
In order to take your first steps toward gaining that confidence
you can follow the same procedures we use here at Biotage to
Part No: AN747Issued year: 2011File size: 0.18mbFile type: pdf
Biotage® PRESSURE+ manifolds deliver positive pressure, parallel processing for 96 well plates and column formats. The systems utilize a consistent, uniform flow of positive pressure to move both low and high viscosity liquids through SPE plates and columns. ISOLUTE SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation and greatly matrix effects associated with biofluid analysis.
SLE+, SLE, Supported Liquid Extraction, positive pressure manifold, Pressure+96, Pressure+48
Part No: UI333Issued year: 2015File size: 0.89mbFile type: pdf
If high back pressure is detected in the makeup pump lines, and/or the High Makeup Flow Pressure dialog appears, the microfilter may be partially clogged and needs replacing. This is a step by step guide.
Part No: UI403Issued year: 2019File size: 0.13mbFile type: pdf
Biotage® Sfär columns can be used with CombiFlash Rf and
CombiFlash NextGen Systems. This document outlines how to
set up your CombiFlash instrument for use with Biotage® Sfär
Silica HC and Biotage® Sfär C18.
Part No: UI319.v3Issued year: 2017File size: 0.7mbFile type: pdf
Biotage®SNAP Ultra cartridges are compatible with CombiFlash® Rf Systems. As a convenience, Biotage SNAP Ultra cartridges with Luer slip connectors are available. Contact your Biotage representative for ordering information.
Part No: TechTipIssued year: 2011File size: 0.07mbFile type: pdf
Are you keeping up with your annual six month Hydrophilic Solution treatments for your SPE-DEX 4790 or 1000/3000 SPE-DEX instruments?
1. The water sample will not flow down into the disk holder.
2. My water inlet valve may not be opening
3. My water inlet valve is opening, but my sample is not dispensing into the disk holder.
4. My entire collection vessel is filled with the water sample.
Part No: AN078Issued year: 2013File size: 1.05mbFile type: pdf
In this application note we demonstrate the power of mass detection in Isolera™ Dalton by investigating the incomplete separation of two dyes, Butter yellow (Mass 225.29 g/mol) and Sudan red (Mass 278.31 g/mol), by normal phase flash purification.
Part No: P053Issued year: 2013File size: 1.22mbFile type: pdf
Immunosuppressant drugs are extremely important for therapeutic drug monitoring (TDM) regimes, requiring robust and reliable methods for their analysis. This poster aims to demonstrate strategies for the extraction of various immunosuppressant drugs from whole blood providing clean samples prior to UPLC-MS/MS analysis. Spiked whole blood was extracted using a variety of protocols to investigate optimal combination of drug recovery and extract cleanliness. UPLC-MS/MS was conducted using a Waters ACQUITY UPLC coupled to a Quattro Premier XE triple quadrupole mass spectrometer. Acceptable extraction recoveries were obtained showing excellent extract cleanliness, demonstrating a reliable method for mass spectrometric approaches for these analytes.
Immunosuppressants, SLE, Supported Liquid Extraction, ISOLUTE, MSACL, San Diego, 2013
Part No: P140Issued year: 2016File size: 0.31mbFile type: pdf
Reversed-phase chromatography is typically used when you need to separate several milligrams of relatively polar compounds that either are not soluble in normal-phase solvents or are not compatible with bare silica
because they react, stick, or both. If you are currently using reversed-phase at preparative scale, such as flash chromatography, you know the mobile phase limitations – water with either methanol, acetonitrile, or
THF. As with normal-phase flash chromatography, when it comes time to purify you want your crude sample fully solubilized in the weakest possible solvent at the highest possible concentration.
Part No: AN044-HORIssued year: 2009File size: 0.65mbFile type: pdf
The Biotage® DryVap Concentrator System was intentionally designed to provide enhanced recoveries of semivolatile organic compounds (SVOC) such as those found in EPA Method 8270. The unique design of the DryVap system, which uses a vacuum, sparge gas, and internal immersion heater, allows it to achieve excellent recoveries for a wide range of compounds.
This purpose of this study is to determine the washing
technique for a DryVap® concentrator tube which
optimizes the recovery of both low and high boiling point
Part No: AN075.v2Issued year: 2013File size: 0.49mbFile type: pdf
In this application note we present a new type of polymeric reverse phase adsorbent that is able to provide improved separations of a range of small chemical compounds and also peptides evaluated side by side to a comparable benchmark DVB-Styrene macroporous resin.
MIP, molecularly imprint resins
Part No: AN124-HORIssued year: 2017File size: 1.71mbFile type: pdf
Semivolatile organic compounds (SVOC) have a variety of chemical properties that have been found to cause harmful effects to both humans and the environment. Accurate measurements are challenging to obtain because SVOCs readily adsorb onto surfaces, and are found in common household items such as cleaning agents, personal care products, electrical components, pesticides, water and food.
Part No: AN095-HORIssued year: 2014File size: 2.85mbFile type: pdf
The global demand for fish as a natural source of fresh animal protein, essential fats, minerals, and vitamins continues to rise with the human population. It is estimated that natural fish resources will not be sustainable, creating the need to increase the available supply of fish through aquaculture.
Part No: AN106-HORIssued year: 2016File size: 1.4mbFile type: pdf
Worldwide, there is great concern for the presence of semi-volatile organic compounds in surface water, groundwater, and drinking water, such as Aldrin, extremely toxic to fish and a persistent organic pollutant. China has increased concern about drinking water as sources of surface and groundwater have become polluted with microbiological, metal and organic contamination
Part No: AN063Issued year: 2012File size: 0.48mbFile type: pdf
Pyrazines are a class of organic molecules often used to provide flavor to foods. They are typically synthesized but some are found in fruits and vegetables, e.g. grapes, bell peppers, peas, asparagus, beetroot, tobacco, and roasted foods. Pyrazine’s heterocyclic chemistry can yield some challenges to their purification due to the various separation kinetics between the compound and silica. Biotage SNAP Ultra.
Part No: AN123-HORIssued year: 2017File size: 2.18mbFile type: pdf
The US EPA monitors a variety of chemicals in water that may cause harm to humans or wildlife in order to minimize exposure. Method 625 was developed by the Office of Science and Technology in the Clean Water program to monitor a large suite of semivolatile chemicals in wastewater for compliance with the National Pollution Discharge Elimination System (NPDES). NPDES is a system of permitting that regulates the characteristics of water that is released into a waterway, defined by industrial category. The permitting levels are set depending on the waterway’s use.
Part No: P160Issued year: 2017File size: 0.24mbFile type: pdf
Although capable of very high resolution, RP-HPLC is often limited by low column loading capacity, therefore demanding a significant time investment for peptide purification. As an alternative strategy, reversed-phase flash chromatography can also be used to purify synthetic peptides. The larger particle size used in flash column chromatography enables much larger loading capacity, thereby significantly reducing the time required for peptide purification.
Part No: PPS375.v1Issued year: 2015File size: 0.3mbFile type: pdf
User report: Flash instruments. Chugai Pharmaceutical uses Biotage flash chromatography products for drug discovery research. When deciding to convert from manual open-column procedures to automated systems, they chose successive generations of Biotage products, ranging from the Flash+® packed column to the Biotage® Horizon, SP1, Isolera™ Spektra, and Isolera™ Dalton automated flash chromatography systems.
Part No: AN041Issued year: 2001File size: 0.16mbFile type: pdf
Normal-phase flash purification is commonly used by organic chemists in pharmaceutical drug discovery and
process development labs. However, for many synthesized products (e.g. peptides, nucleotides and basic
drug candidates) purification on standard flash silica is not an option due to irreversible adsorption, chemical
interaction and/or solubility issues. Reversed-phase flash purification is an excellent solution for these applications. Yet, this technique has been used sparingly because of perceived lower loading capacity, higher
operating pressures and a scarcity of publications addressing reversed-phase flash chromatography.
Part No: AN082-HORIssued year: 2012File size: 0.85mbFile type: pdf
The focus of this Application Note is to illustrate some of the advances which have been made when performing EPA method 525.2 when using Solid Phase Extraction (SPE). It will make use of the Biotage(r) Horizon SmartPrep Automated Cartridge Extraction system set up to run in Bottle Rinse Mode with 6 mL SPE cartridges.
Part No: AN039Issued year: 2002File size: 0.64mbFile type: pdf
For pilot and production scale purification drugs, the use of a large particle-size media is common. Improving the purification throughput is limited by the media’s large particle size. Such a case was recently encountered when a pharmaceutical company attempted to improve the efficiency of a large-scale purification. In their current process, the use of a 350-600 µm polystyrene-type resin resulted in a lengthy purification cycle and low separation efficiency. However, the labile properties of the targeted component required a shorter purification cycle and high purification.