Part No: PPS335Issued year: 2013File size: 0.99mbFile type: pdf
ISOLUTE PLD+ plates remove >99 % of plasma proteins and phospholipids, the main causes of ion suppression, leading to cleaner extracts and increased sensitivity (signal-to-noise
(S/N)) for a broad range of analytes.
Part No: PN424Issued year: 2007File size: 0.15mbFile type: pdf
Protein precipitation is a routinely used sample preparation technique for removal of proteins from biological fluid samples prior to analysis. Protein precipitation in the 96-well format, using filtration for protein removal, is a high throughput alternative to the traditional centrifugation based technique.
Part No: TN-0020.0810Issued year: 2010File size: 1mbFile type: pdf
QuEChERS (pronounced “catchers”) stands for Quick, Easy, Cheap, Effective, Rugged, and Safe. The technique is designed to be simple and involve a minimum number of steps; but still allow for effective clean up of complex samples. The QuEChERS technique involves two stages.
Part No: PPS373.V.1Issued year: 2016File size: 0.91mbFile type: pdf
ISOLUTE® Si-Propylsulfonic acid (SCX-2) belongs to a class of strong supported acids. As a bound sulfonic acid, it has a natural propensity to bind alkali and some transition metal group metals, which means that it can be used as a scavenger for metals with a +I or +II oxidation state, such as Na, K, Li, also
Pd, Rh, and Ru.
Part No: PPS372.V.1Issued year: 2016File size: 2.4mbFile type: pdf
ISOLUTE® Si-Trisamine is a silica bound equivalent of a trisamine base and can scavenge a variety of electrophiles, including aldehydes, acid chlorides, sulfonyl chlorides, isocyanates, isothiocyanates and heavy metal ions such as Mn2+, Fe3+, Co2+, Ni2+, Cu2+, Pb+2, Ru+2 and Zn2+.
Part No: TN0017.0406Issued year: 2006File size: 0.05mbFile type: pdf
ISOLUTE® Si-Propylsulfonic acid (SCX-2) belongs to the class of strong acids. Similar
to ISOLUTE Si-TsOH (SCX-3)1, it can be used to (a) scavenge amines and other bases such
as anilines and borohydrides (b) as an acid catalyst in reactions or (c) as a replacement
for aqueous or organic acids in quenching reactions. The advantage of using SCX-2 as a
bound acid reagent is in that it eliminates work-up. The crude reaction mixture can be evaporated to dryness and the resulting free flowing solid can be purified directly by flash column chromatography.
Part No: TN0018.0406Issued year: 2006File size: 0.09mbFile type: pdf
ISOLUTE® Si-Thiol is the silica-bonded equivalent of 1-propanethiol, which is useful
for covalent scavenging of alkyl, benzyl, and allyl halides as well as a variety of other
electrophiles including acid chlorides and isocyanates. In addition it can be used to
scavenge a variety of metals1 used in organic synthesis including Pd, Pt, Cu, Hg, Ag and Pb.
Part No: PPS374.V.1Issued year: 2016File size: 0.98mbFile type: pdf
ISOLUTE® Si-Thiol is the silica-bound equivalent of 1-propanethiol which is primarily used in the pharmaceutical industry as a metal scavenger for metals such as Pd, Pt, Cu, Hg, Ag and Pb but it can also be useful for covalent scavenging of alkyl, benzyl and allyl halides as well as a variety of other electrophiles including acid chlorides and isocyanates.
Part No: TN0016.0406Issued year: 2006File size: 0.07mbFile type: pdf
ISOLUTE® Si-TsOH (SCX-3) is the bound equivalent of p-toluene sulfonic acid with a
pKa <1. Similar to ISOLUTE propylsulfonic acid (SCX-2)1 , it can be used to (a) scavenge
amines and other bases such as anilines and borohydrides (b) as an acid catalyst in reactions
or (c) as a replacement for aqueous or organic acids in quenching reactions.
Part No: PPS431.v1Issued year: 2016File size: 0.48mbFile type: pdf
At Chiba University’s Graduate School of Medicine and its affiliated hospital, researchers are developing an LC-MS/MS method for vitamin D analysis using ISOLUTE® SLE+ and other Biotage products. We interviewed Professor Mamoru Satoh at the Disease Proteomics Center and Mr. Takashi Ishige from the Inspection Department at the affiliated hospital.
Part No: TN-0011.0708Issued year: 2008File size: 0.34mbFile type: pdf
ISOLUTE® SLE+ Supported Liquid Extraction Plates offer an efficient alternative to traditional liquid-liquid extraction
(LLE) for bioanalytical sample preparation, extracting up to 400 μL of aqueous sample. ISOLUTE SLE+ plates provide
high analyte recoveries, eliminate emulsion formation, and cut sample preparation time in half.
Part No: TN-0012.0610 (2)Issued year: 2010File size: 0.35mbFile type: pdf
ISOLUTE® SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional liquidliquid extraction (LLE) for bioanalytical sample preparation, extracting up to 10 mL of aqueous sample. ISOLUTE SLE+ provides high analyte recoveries, eliminates emulsion
formation and reduces sample preparation time by half.
Part No: AN068Issued year: 2013File size: 0.6mbFile type: pdf
The ability to synthesize peptides in a range of different
scales is a requirement in many research laboratories. The
Biotage® Initiator+ Alstra™ fully automated microwave peptide
synthesizer has a scale range of 5 μmol up to 2 mmol using
three different reactor vial sizes (5 mL, 10 mL and 30 mL).
The acyl carrier protein fragment, ACP (65–74), H-Val-Gln-
Ala-Ala-Ile-Asp-Tyr-Ile-Asn-Gly-NH2 (VQAAIDYING-NH2) (1), is
a well-known so-called difficult sequence and is commonly
used to evaluate the performance of new synthesis reagents
Here we demonstrate a variable scale synthesis on the
Initiator+ Alstra using the ACP (65–74) fragment as a model
Part No: PPS397Issued year: 2016File size: 0.23mbFile type: pdf
Customer Case: Satori Pharmaceuticals. Extracting target compounds from large volumes of mixture confronts chemists with challenges which are right at the heart of large scale process chemistry. Ruichao Shen at Satori Pharmaceuticals knows exactly what it takes.
Part No: P115Issued year: 2014File size: 2.69mbFile type: pdf
This poster compares the extraction of the ethanol metabolites (alcohol biomarkers) from urine using strong anion exchange (EVOLUTE AX) and weak anion exchange (ISOLUTE NH2) solid phase extraction columns. A comparison with a simple dilution protocol is also presented.
Part No: P173Issued year: 2018File size: 0.55mbFile type: pdf
Urine drug testing to support pain management is a mainstay of the clinical toxicology laboratory. Reduced reimbursement has continued to put increasing pressure on laboratories. Many toxicology labs are moving to larger drug panels to increase throughput and efficiency, while reducing turn-around-time and cost.
Part No: PPS526Issued year: 2015File size: 0.68mbFile type: pdf
In the laboratory many chemicals are used in small and larger volumes. Table 1 shows several of the typical kinds of analyses done for environmental compliance. Semivolatile organic compound extraction and evaporation can be a large source of solvent emission to the atmosphere if the solvent is allowed to go up the hood and is not recaptured.
Part No: AN064Issued year: 2012File size: 1.09mbFile type: pdf
This application note describes the use of ISOLUTE® HM-N as a support material
for pre-adsorption of a reaction mixture prior to flash chromatography. This
approach is useful when loading reaction mixtures that are only soluble in polar
solvents onto a Biotage® SNAP Ultra, SNAP KP-SIL or SNAP KP-NH column.
Part No: AN128Issued year: 2019File size: 0.27mbFile type: pdf
In this application note, we compare the amounts of a threecomponent
mixture that can be separated on columns of the
same size, 25 g, but with different Silica material: SNAP KP-Sil
(average 50 μm irregular particles), Sfär Silica (60 μm spherical
particles) and Sfär Silica HC (20 μm spherical particles) using a
Biotage® Selekt instrument.
Part No: P211Issued year: 2019File size: 1.16mbFile type: pdf
This poster compares manual SPE (EVOLUTE EXPRESS CX) processing using the Biotage Pressure+ 96 with automated processing using Biotage Extrahera, using a suite of 98 drugs of abuse analytes in whole blood . Parameters such as the total time for extraction of 96 samples, and consistency of analyte recovery and RSD are explored.
Part No: TechTipIssued year: 2011File size: 0.15mbFile type: pdf
The focuses of this month’s discussion are the:
“O” ring (30-0452-01) on the bottom of any 1000/3000 Disk Holder.
Cap Adapters (150-0001, 150-0008-01)
40 mL VOA vial adapter (160-0001-02)
125 mL Sepratory Funnel (27-0834-01)
Rinse Rods (50-0667)
Gasket on the top of the water in valve (02-0560)
“O” Rings used on the Rinse Rods – (30-0544, bag of 100).
Part No: AN108Issued year: 2015File size: 0.38mbFile type: pdf
In this study we synthesized two closely related amphipathic peptides and show the mass directed flash purification of non-acetylated ‘18A’ from a crude mixture containing a single residue deletion sequence ‘17A’ (-Glu residue) using the Biotage® Isolera Dalton™.
Part No: AN109Issued year: 2015File size: 0.71mbFile type: pdf
Here we show how flash chromatography with mass directed fractionation can be used for purifying carbohydrate derivatives such as β-D-glucosamine pentaacetate 1 as a model compound, and other UV transparent or poorly UV absorbing compounds using either normal or reversed-phase chromatography.
Part No: AN077Issued year: 2013File size: 1.35mbFile type: pdf
Developed by collaboration with the University of Eindhoven, European Regional Marketing and R+D, this application note describes the application of Isolera Dalton to the detection of multiply charged peptide ions prior to purification. This effectively increases the range of the Isolera Dalton mass detection, for this class of compounds and demonstrates that Isolera Dalton is a fully integrated purification instrument rather than a simple MS based analytical tool.
Part No: P098Issued year: 2014File size: 0.28mbFile type: pdf
In this poster we will show how using reversed-phase flash chromatography with the new Biotage® Isolera™ Dalton mass-directed flash purification system provides real-time simultaneous separation, detection, and identification of lipids.
Presented at ACS Spring 2014.