Part No: AN068Issued year: 2013File size: 0.6mbFile type: pdf
The ability to synthesize peptides in a range of different
scales is a requirement in many research laboratories. The
Biotage® Initiator+ Alstra™ fully automated microwave peptide
synthesizer has a scale range of 5 μmol up to 2 mmol using
three different reactor vial sizes (5 mL, 10 mL and 30 mL).
The acyl carrier protein fragment, ACP (65–74), H-Val-Gln-
Ala-Ala-Ile-Asp-Tyr-Ile-Asn-Gly-NH2 (VQAAIDYING-NH2) (1), is
a well-known so-called difficult sequence and is commonly
used to evaluate the performance of new synthesis reagents
Here we demonstrate a variable scale synthesis on the
Initiator+ Alstra using the ACP (65–74) fragment as a model
Part No: PPS397Issued year: 2016File size: 0.23mbFile type: pdf
Customer Case: Satori Pharmaceuticals. Extracting target compounds from large volumes of mixture confronts chemists with challenges which are right at the heart of large scale process chemistry. Ruichao Shen at Satori Pharmaceuticals knows exactly what it takes.
Part No: P115Issued year: 2014File size: 2.69mbFile type: pdf
This poster compares the extraction of the ethanol metabolites (alcohol biomarkers) from urine using strong anion exchange (EVOLUTE AX) and weak anion exchange (ISOLUTE NH2) solid phase extraction columns. A comparison with a simple dilution protocol is also presented.
Part No: PPS526Issued year: 2015File size: 0.68mbFile type: pdf
In the laboratory many chemicals are used in small and larger volumes. Table 1 shows several of the typical kinds of analyses done for environmental compliance. Semivolatile organic compound extraction and evaporation can be a large source of solvent emission to the atmosphere if the solvent is allowed to go up the hood and is not recaptured.
Part No: AN064Issued year: 2012File size: 1.09mbFile type: pdf
This application note describes the use of ISOLUTE® HM-N as a support material
for pre-adsorption of a reaction mixture prior to flash chromatography. This
approach is useful when loading reaction mixtures that are only soluble in polar
solvents onto a Biotage® SNAP Ultra, SNAP KP-SIL or SNAP KP-NH column.
Part No: AN128Issued year: 2019File size: 0.27mbFile type: pdf
In this application note, we compare the amounts of a threecomponent
mixture that can be separated on columns of the
same size, 25 g, but with different Silica material: SNAP KP-Sil
(average 50 μm irregular particles), Sfär Silica (60 μm spherical
particles) and Sfär Silica HC (20 μm spherical particles) using a
Biotage® Selekt instrument.
Part No: TechTipIssued year: 2011File size: 0.15mbFile type: pdf
The focuses of this month’s discussion are the:
“O” ring (30-0452-01) on the bottom of any 1000/3000 Disk Holder.
Cap Adapters (150-0001, 150-0008-01)
40 mL VOA vial adapter (160-0001-02)
125 mL Sepratory Funnel (27-0834-01)
Rinse Rods (50-0667)
Gasket on the top of the water in valve (02-0560)
“O” Rings used on the Rinse Rods – (30-0544, bag of 100).
Part No: AN108Issued year: 2015File size: 0.38mbFile type: pdf
In this study we synthesized two closely related amphipathic peptides and show the mass directed flash purification of non-acetylated ‘18A’ from a crude mixture containing a single residue deletion sequence ‘17A’ (-Glu residue) using the Biotage® Isolera Dalton™.
Part No: AN109Issued year: 2015File size: 0.71mbFile type: pdf
Here we show how flash chromatography with mass directed fractionation can be used for purifying carbohydrate derivatives such as β-D-glucosamine pentaacetate 1 as a model compound, and other UV transparent or poorly UV absorbing compounds using either normal or reversed-phase chromatography.
Part No: AN077Issued year: 2013File size: 1.35mbFile type: pdf
Developed by collaboration with the University of Eindhoven, European Regional Marketing and R+D, this application note describes the application of Isolera Dalton to the detection of multiply charged peptide ions prior to purification. This effectively increases the range of the Isolera Dalton mass detection, for this class of compounds and demonstrates that Isolera Dalton is a fully integrated purification instrument rather than a simple MS based analytical tool.
Part No: P098Issued year: 2014File size: 0.28mbFile type: pdf
In this poster we will show how using reversed-phase flash chromatography with the new Biotage® Isolera™ Dalton mass-directed flash purification system provides real-time simultaneous separation, detection, and identification of lipids.
Presented at ACS Spring 2014.
Part No: AN085Issued year: 2014File size: 0.92mbFile type: pdf
Mass-directed flash purification is a simple and effective
technique to detect and fractionate compounds with poor UV chromophores. In this study, fatty acid methyl ester mixtures were purified by mass-directed flash purification using the Biotage Isolera™ Dalton System.
Part No: P099Issued year: 2014File size: 0.39mbFile type: pdf
In this poster we demonstrate real-time detection and identification of peptides during reversed-phase flash chromatography with the new Biotage® Isolera™ Dalton mass-directed flash purification system.
Presented at ACS Spring 2014.
Part No: P168Issued year: 2017File size: 0.28mbFile type: pdf
Natural product chemistry deals with discovering the previously unknown in nature. Compounds found in nature are typically found in low quantity and thus extractions are needed to isolate certain compounds classes or at least compounds with similar solubility.
Part No: PN38Issued year: 2011File size: 0.17mbFile type: pdf
Mephedrone (MCAT) is a ‘designer drug’ synthesised to mimic the amphetamine class of compounds. Due to its perceived ‘ecstasy like’ effects of euphoria, excitement and alertness combined with ease of availability, this (until recently) unregulated drug (legal high) has found widespread abuse in the UK in recent years. Harmful effects associated with mephedrone are still being studied but after several deaths were linked to the abuse of the drug, the UK Government made this a Class B substance. Mephedrone extraction was evaluated on three mixed mode cation exchange SPE sorbents: EVOLUTE® CX, EVOLUTE® WCX and ISOLUTE® HCX.
DOA, ISOLUTE, EVOLUTE, Amphetamine
Part No: Issued year: 2011File size: 0.44mbFile type: pdf
User Report: Metal scavenger Si-Thiol. Nippon Shinyaku Co., Ltd use Biotage metal scavengers to control residual metals such as palladium, which are difficult to remove by other methods. We have been talking to Mr. Toshio Fujiwara who is in charge of Process Chemistry, CMC Research & Development Department, Discovery Research Laboratories.
Part No: P148Issued year: 2016File size: 0.93mbFile type: pdf
Biotage metal scavengers are a more effective alternative to traditional carbon batch adsorption processes. They have the lowest metals content, and also excellent extractable profiles, therefore not likely to risk contamination, unlike competitive materials we tested. Furthermore, Biotage metal scavengers offer volumetrically, a much more compact solution than carbon, requiring 48 times less volume for the same degree of Pd removal in our studies.
Part No: AN102Issued year: 2015File size: 1.12mbFile type: pdf
This study was conducted in 3 parts.
Metal scavengers were investigated for inherent metals content by ICP analysis methods.
Additionally these scavengers were extracted in a range of common solvents in order to show extractable/leachable levels.
We demonstrate an example metal scavenging mitigation step, looking at the corresponding API mass yield, purity and also metals data.
Part No: P187Issued year: 2018File size: 0.29mbFile type: pdf
Recently there has been substantial motivation to consider and evaluate alternative, more environmentally friendly solvents. Some countries have even gone so far as to ban some of the more toxic, yet commonly used solvents. In addition to general toxicity, the volume of solvent used in any particular application is given extra consideration in the green chemistry movement. In this regard, purification solvent selection is closely monitored as they are often
used in large quantities.
Part No: AN007-HORIssued year: 2009File size: 0.64mbFile type: pdf
EPA Method 515.2 is applicable to the determination of
chlorinated acids in ground water and finished drinking
water. The form of each acid is not distinguished by this
method. Results are calculated and reported for each listed analyte as the total free acid. The analytes are extracted from the water using a DVB-Hydrophobic disk and the Horizon Technology SPE-DEXÒ 4770 Automated
Extraction System. The disk is extracted with Methylene
Chloride and the extract dried with sodium sulfate. The
extract is derivatized with diazomethane. Final analysis is by GC/ECD.
Part No: AN056-HORIssued year: 2015File size: 1.24mbFile type: pdf
One of the most frequently desired quantities in environmental chemistry is the amount of diesel range organics (DROs) contained within a sample. The analysis of DROs is performed in almost every major environmental laboratory under the US EPA Method 80151 or by using the similar ISO 9377-22. This method is commonly used to determine the contamination of alkanes from C10 to C28 caused by leaking underground storage containers.
Part No: PPS522Issued year: 2014File size: 0.36mbFile type: pdf
SmartPrep automated cartridge extractor is the key to working through the method development process. SPE automation only requires the user to setup development parameters in the software and the instrument does the rest. While the SmartPrep takes care of the SPE method development steps, an LC or LC/MS method can be developed.
Part No: TN104.V.1Issued year: 2017File size: 0.88mbFile type: pdf
The ISOLUTE® family of anion exchange sorbents are used to extract organic anions (acidic compounds capable of exhibiting a negative charge) from both aqueous and non-aqueous matrixes.
Anion exchange SPE can be accomplished by strong (very high pKa) or weak (lower pKa) ion exchangers.
Part No: TN106.V.1Issued year: 2017File size: 0.9mbFile type: pdf
The ISOLUTE® family of cation exchange sorbents are used to extract organic cations (basic compounds capable of exhibiting a positive charge) from both aqueous and non-aqueous matrixes. Although extraction is by the same mechanism, each sorbent has properties that influence the way they are used.