Part No: P077Issued year: 2014File size: 0.48mbFile type: pdf
This poster uses gel electrophoresis data to visually illustrate the amount of residual protein in extracts prepared using protein precipitation, supported liquid extraction and various silica and polymer based SPE approaches.
Part No: TN125Issued year: 2003File size: 0.07mbFile type: pdf
The ISOLUTE HCX series of mixed-mode SPE sorbents is based on a combination of strong cation exchange and non-polar chemistries. Basic drugs are retained by two primary retention mechanisms. This allows a rigorous interference elution regime to be used to elute interferences retained by either non-polar or cation exchange interactions alone. Only analytes with both non-polar and basic characteristics are extracted using the ISOLUTE HCX series of sorbents, providing an extremely pure final extract.
Part No: TN126Issued year: 2003File size: 0.06mbFile type: pdf
ISOLUTE non-polar SPE sorbents retain drugs from aqueous biological fluids through hydrophobic interactions. Endogenous compounds from the original sample matrix, such as proteins, are not retained. When the sorbent is rinsed, weakly retained compounds are eluted to waste, leaving strongly retained compounds to be eluted in the final elution solvent.
Part No: AN898Issued year: 2018File size: 1.91mbFile type: pdf
This application note describes a procedure for sample
pre-treatment and extraction of a panel of 49 drugs of
abuse from human hair, using Biotage® Lysera for matrix
micropulverization, prior to clean up using ISOLUTE® SLE
supported liquid extraction. The application note contains
procedures optimized for 400 μL capacity column format and
200 μL and 400 μL supported liquid extraction plate formats,
for higher throughput applications. The methodology delivers
clean extracts and analyte recoveries mostly greater than
80% with RSDs lower than 10% for all analytes and LLOQ from
Part No: P165Issued year: 2017File size: 0.32mbFile type: pdf
This poster evaluates 3 different sample preparation approaches (ISOLUTE SLE+, EVOLUTE EXPRESS ABN, EVOLUTE EXPRESS CX) for extraction of large multi-drug urine panels.
Each approach is assessed in terms of suitability for extraction of analytes with different different properties (pka, LogP etc).
Part No: Issued year: 2007File size: 0.48mbFile type: pdf
•Extends the range of acidic, basic and neutral compounds that can be extracted using a single generic method, increasing method development productivity•Has an optimized pore structure which reduces the extraction of endogenous matrix components, and leads to cleaner extracts that give fewer matrix effects in LC-MS/MS analysis•Has optimized physical and chemical characteristics that improve reliability of SPE procedures•Has no secondary interactions, allowing the use of pure organic elution solvent to give high recoveries of very low drug concentrations
Part No: TN127Issued year: 2003File size: 0.28mbFile type: pdf
ISOLUTE HAX mixed-mode SPE sorbent is based on a combination of strong anion exchange and non-polar chemistries. Acidic drugs are retained by two primary retention mechanisms. This allows a rigorous interference elution regime to be used to elute interferences retained by either non-polar or anion exchange interactions alone. Only analytes with both non-polar and acidic characteristics are extracted usign the ISOLUTE HAX sorbent, providing an extremely pure final extract.
Part No: Issued year: 2004File size: 0.31mbFile type: pdf
1.We have developeda general method for synthesizing Diaryl Ethers from both electron richand electron deficient phenols.
2.We have showedthat with our instrumentation we have removedscalabilityas alimiting factor for the ”bench” chemist.
3.We have also developeda new cooling technique on the Advancer that dramaticallyshortens coolingtimes. For ethanol: 180 ºC to 70 ºC within less than 10 seconds..
Part No: P020Issued year: 2008File size: 0.16mbFile type: pdf
The trend pharma industry is clearly to speed up the process from lead identification to selection of candidate drug. One of the major limitations is the availability of larger amounts typically hundreds of grams, of the potentially active substance to be further investigated
Part No: P153.v.1Issued year: 2017File size: 1.12mbFile type: pdf
This poster describes a simple but effective method for screening a range of pesticides and drugs of abuse from liver samples. Liver tissue is homogenized using the Biotage® Lysera, and the subsequent extract is cleaned up using ISOLUTE SLE+ supported liquid extraction columns.
Part No: P012Issued year: 2006File size: 0.22mbFile type: pdf
The use of palladium-catalysts in the synthesis of fine chemicals, pharmaceutical intermediates and active pharmaceutical ingredients (APIs) has become quite common in the last few decades. The number of palladiumcatalyzed reactions (both achiral and chiral) available to chemists has provided access to more complex structures in fewer steps and with less waste. An unfortunate side effect of using palladium is the potential for palladiumcontaining impurities to remain in the desired compound after isolation. This is an especially significant problem for the pharmaceutical industry since there is a low limit for heavy metal impurities allowed in the drug substance.
Part No: AN072.v2Issued year: 2013File size: 0.39mbFile type: pdf
Here we present the separation capabilities of a new type of designed adsorbent, RENSA® Boronate. This new resin has affinity for diols and α-hydroxycarboxylic acids and is able to extract those target compounds with high efficiency and selectivity.
Part No: Issued year: 2010File size: 0.26mbFile type: pdf
• Solid-Phase Peptide Synthesis (SPPS) is still often
faced with difficulties in the assembly of long and
‘difficult’ sequences, e.g. due to aggregation and
steric hindrance giving rise to incomplete reactions.
These problems have only partly been solved by
new coupling reagents and solid supports.
• Precise microwave heating has emerged as one
new parameter for SPPS, in addition to coupling
reagents, resins, solvents etc.
Part No: Issued year: 2017File size: 0.31mbFile type: pdf
This poster includes a simple Load-Wash-Elute solid phase extraction method using EVOLUTE EXPRESS WCX, for extraction of aminoglycoside antibiotics from a range of meat, milk and egg samples.
The sample preparation method is automated using Biotage Extrahera, with fast, sensitive analysis utilizing HILIC-MS/MS with MRM Spectrum Mode for ID confirmation (Shimadzu LCMS-8060).
Part No: AN091Issued year: 2014File size: 0.63mbFile type: pdf
Three protected carbohydrate derivatives, 1,2:5,6-Di-O-Isopropylidene-
α-D-glucofuranose, Methyl 4,6-O-Benzylidene-α-D-glucopyranose, and
1,2-O-Isopropylidene-α-D-glucofuranose were successfully separated and
detected, using a Biotage® SNAP KP-Sil cartridge on a Biotage Isolera™ system
with the Biotage® Evaporative Light-Scattering Detector (ELSD) attached.
Part No: AN089Issued year: 2014File size: 0.68mbFile type: pdf
Three sterols, (+)-dihydrocholesterol, β-estradiol-3-benzoate, and
β-estradiol were successfully separated, detected, and isolated using a
Biotage® SNAP KP-Sil cartridge attached to a Biotage Isolera™ One system connected to Biotage® Evaporative Light-Scattering Detector (ELSD).
Part No: TechTipIssued year: 2011File size: 0.06mbFile type: pdf
There are many things that can cause your Speed Vap III to have a problem with the vacuum. Such as the following examples:
1. The compressed air has to flow through the Speed Vap, if there is no exhaust, there is no vacuum. (The exhaust contains solvent vapors and should be properly vented)
2. There may not be sufficient compressed air being delivered to the Speed Vap. (2 CFM is required)
3. If the unit is connected to a Solvent Trap, the rubber stopper that goes into the collection flask may not be making a good seal.
4. The seal on Speed Vap III lid compresses over time and it will need to be replaced to maintain a good seal.
Part No: Issued year: 2007File size: 3.19mbFile type: pdf
Biotage bietet ein umfassendes Jahresservice-Konzept, inhaltlich aller Ersatzteile, Arbeit vor Ort und empfohlener vorbeugender Wartung. Holen Sie sich den Prioritätsstatus für alle Service-Anrufe durch spezialisierte Außendiensttechniker von Biotage und mit
Original-Biotage-Ersatzteilen (wesentlich zur Aufrechterhaltung der optimalsten Geräteleistung).
Part No: Issued year: 2007File size: 0.05mbFile type: pdf
PS-TsNHNH2 and ISOLUTE® Si-TsNHNH2 are solid supported equivalents of p-toluenesulfonyl hydrazide. These reagents are both excellent scavengers for aldehydes and ketones and are generally used in threefold excess with respect to the limiting carbonyl species. A catalytic amount of acetic acid (5-10%) may be required for ketones, hindered substrates or for scavenging in certain solvents (e.g. DMF).
Part No: P022Issued year: 2008File size: 0.09mbFile type: pdf
Microwave-assisted esterification of protected amino acid derivatives was performed using alkyl halides and polystyrene supported TBD1,2 (Fig. 1) as base. The preferred solvents were THF or acetonitrile. The products were obtained in good yields and high purity. Short reaction time, 10 minutes, and easy work-up, filtration and evaporation, constituted a convenient procedure. Benzyl, allyl, phenacyl, and alkyl esters, all important and commonly used derivatives in peptide synthesis, were synthesised.
Part No: AN092-HORIssued year: 2014File size: 1.59mbFile type: pdf
The United States Food & Drug Administration (FDA) regulates the approval of color additives for their safe use in foods, cosmetics, pharmaceuticals/drugs, and medical devices. The Federal Food, Drug, and Cosmetic Act (FD&C Act) contains provisions for what color additives are required to be certified. These provisions are monitored by the FDA, and through the Code of Federal Regulations (CFR), the FDA releases information on new color additives and new uses for certified color additives.
Part No: AN894Issued year: 2018File size: 1.17mbFile type: pdf
This application note describes the simultaneous extraction of aldosterone at low (< 25pg/mL) concentration and angiotensin I and II from plasma, with the potential to improve throughput and reduce time and costs in PRA determination.
Part No: P155Issued year: 2017File size: 0.61mbFile type: pdf
This poster discusses the impact of optimization of various parts of the method development process to maximize assay performance while delivering a combined assay for the analysis of urinary catecholamines and metanephrines. The extraction method using EVOLUTE EXPRESS WCX 96-well plates is successfully automated using the Biotage® Extrahera™ Automated Sample Preparation Platform.
MSACL 2017, Palm Springs
ASMS 2017, Indianapolis
Part No: AN755Issued year: 2012File size: 1.03mbFile type: pdf
Ethyl glucuronide (EtG) and ethyl sulphate (EtS) are specific metabolites formed within the body after the ingestion of alcohol. In forensic toxicology, when tested together they provide more specific evidence of alcohol intake. This application note demonstrates the simultaneous extraction of both EtG and EtS in the same assay and represents further optimization of AN718 which showed single EtG extraction without EtS. Recoveries for EtG range from 90-105% and 80-103% for EtS across two formats of EVOLUTE AX columns with RSDs <10%. The LOQ is 10 ng/mL for EtG and 2 ng/mL for EtS across both EVOLUTE AX formats.
EVOLUTE AX, EVOLUTE, EtS, EtG, Ethyl Glucuronide, Ethyl Sulfate, SPE, Anion Excange, Urine, OASIS, Waters, Polymeric, Polmers,
Part No: AN098Issued year: 2014File size: 0.81mbFile type: pdf
To demonstrate the cost savings by employing small μmol scale automated solid phase peptide synthesis through robot liquid handling of reagents with accurate dispensing of small volumes, a 10-mer antimicrobial peptide was synthesized.
Part No: AN073-HORIssued year: 2012File size: 0.79mbFile type: pdf
Solid Phase Extraction (SPE) cartridges have been used for many years, and are well established as a proven extraction,
clean-up, and fractionation technique for a wide variety of
sample matrices. As such, a diverse set of chemistries are
available to handle these various samples. For all of this
work, cartridges can be used manually, via a manifold-type
setup, or with an automated system, which would simply
automate the manual steps.
Part No: AN071-HORIssued year: 2012File size: 0.79mbFile type: pdf
Based on samples to be processed, the final analytical technique to be employed, or the need to develop and optimize an analytical extraction method, there are times when it is desirable to collect multiple elution fractions from a single sample.
Part No: AN070-HORIssued year: 2012File size: 0.78mbFile type: pdf
Analytical methods which extract aqueous samples require
that the initial volume of the sample be determined, as it is necessary to calculate the concentration of the analytes in the sample. Typically, this is done by marking the sample
meniscus on the side of the sample bottle before the sample
Part No: PN0021407_02Issued year: 2014File size: 0.78mbFile type: pdf
Automating Solid Phase Extraction (SPE) can be simple and efficient with the SmartPrep Extractor. Designed for laboratories transitioning from manual to automated SPE, the SmartPrep Extractor focuses on the essential SPE process to run a variety of sample matrices and volumes without dedicated experts.
Part No: PN0031406-01Issued year: 2014File size: 3.01mbFile type: pdf
Solid Phase Extraction (SPE) is one of the most common sample preparation techniques that can be automated with simplicity. The SmartPrep Extractor Software offers an easy and intuitive interface specifically designed to streamline the manual SPE process for 1, 3, and 6-mL cartridges. No specialized training or expertise is needed to run samples. Any technician level from novice to expert can quickly get started with SPE sample processing.
Part No: B0061605_01bIssued year: 2016File size: 4.96mbFile type: pdf
The SmartPrep II Cartridge System is
designed for simple automation of
manual solid phase extraction (SPE)
methods. Just like manual SPE, up to
12 samples per SmartPrep II System
can be run in one batch.
Part No: PN0011407_02Issued year: 2014File size: 0.8mbFile type: pdf
Solid phase extraction (SPE) of transformer oils, biofuels, edible, and essential oils can offer challenges of working with difficult matrices, multiple methods, and hazardous solvents.
The SmartPrep Oil & Fuel Extractor systems are complete with racks, sample tubes, and accessories packaged to automate the typical manual SPE oil and fuel procedures, removing the challenges of working with oils and fuels, while providing a consistent and simple approach to running multiple methods on the same platform.
Part No: Issued year: 2008File size: 0.13mbFile type: pdf
The adapter kit contains a pre-assembled stand, spacer and
connecting tubing; everything required to allow the use of
SNAP 340g cartridges on flash purification systems that utilize
Luer tubing connections such as, Isco Companion and Analogix
Part No: install_snap_25.zipIssued year: 2012File size: 0mbFile type: zip
7742_install_snap_25.zip. SPX 25 g SNAP cartridge installation.
The system already has the SNAP 10 g, 50 g, 100 g and 340 g cartridges installed
(only the SNAP 25 g needs to be installed)
Part No: install_snap_full.zipIssued year: 2012File size: 0mbFile type: zip
install_snap_full.zip. SNAP Software Update for Biotage® SP Systems. SPX FULL SNAP cartridge installation instructions.
The systems that does not have any SNAP cartridges installed
(the SNAP 10 g, 25 g, 50 g, 100 g and 340 g will be installed)