Part No: AN060-HORIssued year: 2010File size: 1.33mbFile type: pdf
This purpose of this study is to determine how the Directto- GC vial concentrator tube and the DryVap® Concentrator System can optimize the recoveries for both low and high boiling point semi-volatile compounds. A spiking mixture from EPA method 8270D was chosen due to the more volatile compounds in the mix that are difficult to retain during the concentration and subsequent rinsing process; while the higher boiling compounds are more likely to adsorb onto the glass walls.
Part No: AN084Issued year: 2014File size: 0.86mbFile type: pdf
This application note outlines how to use the Isolera system to perform a size-exclusion separation as part of a sample clean up method defined by the EPA, a key sample preparation step in environmental laboratories.
Part No: AN028-HORIssued year: 2010File size: 0.8mbFile type: pdf
Method 1668A is used for determining the level of chlorinated biphenyl congeners by high resolution gas chromatography with high resolution mass spectrometry (HRGC/HRMS). For aqueous samples (samples containing less than 1% solids) – Stable isotopically labelled analogs of the toxic PCB’s are spiked into a 1L sample, and the sample is extracted using the Biotage® Horizon 4790 Automated Extraction System along with the Atlantic™ C18 solid phase extraction (SPE) disk.
Samples are then dried and concentrated using the DryVap® Automated Drying and Concentrating System with DryDisk® technology.
Part No: AN005-HORIssued year: 2009File size: 0.8mbFile type: pdf
EPA Method 506 is used to determine Phthalate and Adipate Esters in drinking water. The analytes are extracted from water using a Horizon Technology 47 mm Atlantic C18 disk. The disk is extracted with Acetonitrile and Methylene Chloride. The extract is then dried and concentrated to a final volume of 1.0 mL using the Horizon Technology DryVap® with DryDisk® technology. Final analysis is by GC/PID.
Part No: AN006-HORIssued year: 2009File size: 0.8mbFile type: pdf
Method 508.1 is used to determine twenty-nine chlorinated
pesticides, three herbicides, and four organohalides in
ground water, drinking water, and water in any treatment
stage. The analytes are extracted from the water using a 47
mm C18 disk. The disk is extracted on the Horizon
Technology SPE-DEX® 4790 Automated Extraction
System using Ethyl Acetate and Methylene Chloride
(DCM). The extract is then dried and concentrated using
the Horizon Technology DryVap® with DryDisk®
technology. Final analysis is by GC/ECD.
Part No: AN010-HORIssued year: 2009File size: 0.8mbFile type: pdf
Method 526 is a gas chromatography / mass spectrometry(GC/MS) method for the determination of selected semivolatile compounds in raw and finished drinking waters.This method efficiently extracts analytes using the SPEDEX® 4790 with an Atlantic® 47 mm polystyrene divinylbenzene (DVB) disk. The disk is extracted with using Ethyl Acetate (EtAc) and Methylene Chloride (DCM). The extract is then dried and concentrated using the DryVap® concentrator system coupled with DryDisk® technology to a final volume of 1.0 mL. Final analysis is done by GC/MS.
Part No: AN013-HORIssued year: 2009File size: 0.74mbFile type: pdf
EPA Method 548.1 details the procedure for the determination of endothall in drinking water by ionexchange solid phase extraction (SPE), acidic methanol methylation, and gas chromatography / mass spectrometry.
This note presents data from the State of Idaho Bureau of Labs for the initial demonstration of capability using the Biotage® Horizon T4790 Automated Extractor System.
Part No: AN015-HORIssued year: 2009File size: 0.79mbFile type: pdf
EPA Method 552.1 is an ion exchange procedure used for determining haloacetic acids and dalapon in drinking water and drinking water sources.
The purpose of this application note is to present data demonstrating the capability of the Horizon Technology SPE-DEX® 4790 Automated Extraction System to perform the sample extraction required for this method.
Part No: P128Issued year: 2015File size: 0.26mbFile type: pdf
In all areas of analytical laboratory testing it is vital to ensure proper quality measures are in place to reduce or eliminate cross contamination between samples, which could result in false positive and/or false negative results. In many cases sample carryover in the LC/MS system is checked early on in the method development process. However, one area that can often be overlooked the sample preparation stage. This involves all aspects including pipetting, sample transfer, extraction protocol, evaporation and mixing steps. This poster examines various stages of the sample preparation process to determine the potential for cross contamination and present approaches to minimize and or eliminate the effect. This is demonstrated via a series of dye experiments combined with analyte testing using LC-MS/MS.
Part No: P126Issued year: 2015File size: 0.48mbFile type: pdf
This poster demonstrates the use of a novel protein and phospholipid depletion plate, for the extraction of 25-hydroxy vitamin D from serum. The extraction protocol was ultimately transferred to an SPE automation platform and method performance versus manual processing was compared.
Part No: P079Issued year: 2014File size: 0.59mbFile type: pdf
The new ISOLUTE® PLD+ Protein and Phospholipid Removal Plate is highlighted in this poster. Protein and phospholipid removal, and analyte recovery are included, along with data illustrating the positive impact of clean (PL and protein free) samples in maintaining analyte signal intensity and low UPLC column back pressure over multiple LCMS runs.
Part No: P088Issued year: 2014File size: 1.06mbFile type: pdf
This poster evaluates the performance of a novel 96-well filter plate (ISOLUTE PLD+ Protein and Phospholipid Removal Plate) for
the simultaneous removal of proteins and phospholipids from serum samples prior to LC-MS/MS analysis.
Part No: P104Issued year: 2014File size: 0.77mbFile type: pdf
This poster presents the ISOLUTE PLD+ Protein and Phospholipid removal plate, highlighting its ease of use and excellent performance with respect to sample clean up, analyte recovery and elimination of back pressure build up in the UPLC system.
Part No: AN100-HORIssued year: 2015File size: 3.44mbFile type: pdf
Disinfection by-products (DBP) are an ever-present nuisance in the efforts to purify drinking water, wastewater and municipal waters from various sources. An emerging class of DBP compounds with health effects is nitrosamines1-3 which result from chloramination or chlorination if the water is nitrogen-rich.
Part No: P153Issued year: 2016File size: 0.52mbFile type: pdf
In postmortem cases, where drugs or pesticides have been used for
their poisonous properties, traditional matrices such as urine and
whole blood may be inappropriate for qualitative and quantitative
analysis. As the site of metabolism for most drugs and toxins, the
liver may provide more insight to cause of death than other bodily
This poster describes the use of ISOLUTE SLE+ supported liquid extraction columns to extract a range of drug and pesticide classes form homogenised liver using a simple, streamlined workflow.
Part No: P112Issued year: 2014File size: 1.4mbFile type: pdf
This poster demonstrates the extraction of a range of drugs of abuse from oral fluid, collected with common collection devices, prior to UPLC-MS/MS analysis. The target analyte list includes benzodiazepines, z drugs, amphetamines, cathinones, opiates, cocaine, buprenorphine, PCP, THC-COOH, fentanyl and ketamine.
Part No: P132Issued year: 2015File size: 1.55mbFile type: pdf
This poster demonstrates the extraction of a range of drugs of abuse from oral fluid collection devices using supported liquid extraction suitable for UPLC-MS/MS analysis. Unlike some sample preparation techniques, SLE allows for the simultaneous extraction of cross-functional analytes in a single extraction protocol without forfeiting extract cleanliness.
The target analyte list includes benzodiazepines, z drugs, amphetamines, cathinones, opiates, cocaine, buprenorphine, PCP, THC-COOH, fentanyl and ketamine.
Part No: P087Issued year: 2014File size: 0.94mbFile type: pdf
This poster describes the extraction of a range of drugs of abuse (including barbiturates, THC and metabolites, benzodiazepines, z drugs, amphetamines,cathinones, opiates, cocaine, buprenorphine, PCP, fentanyl and ketamine) from oral fluid using supported liquid extraction (ISOLUTE SLE+) columns prior to GC-MS and LC-MS/MS analysis.
Part No: P138Issued year: 2015File size: 0.82mbFile type: pdf
This poster demonstrates a fast, reliable protocol to extract multiple drug of abuse panels from whole blood using a common supported liquid extraction methodology. This benefits laboratory workflow where multiple assays are run each day, saving both worker hours and