Part No: AN078-HORIssued year: 2015File size: 1.25mbFile type: pdf
This application note was developed to demonstrate the extraction of five organophosphate compounds Monocrotophos, Diazinon, Malathion, EPN, and Methamidophos using one solid phase extraction method with one pre-treatment step of sodium chloride (NaCl). The method uses the Biotage® Horizon 4790 automated SPE extraction system. It will show the efficiency of the extraction while demonstrating excellent recoveries of OPP compounds using methylene chloride and minimal amounts of acetone after sample pre-treatment with sodium chloride. Methods were developed and results are shown using 47 mm Atlantic® HLB-H disks and carbon cartridges.
Part No: P201Issued year: 2019File size: 0.9mbFile type: pdf
An extraction protocol using a 10 mg mixed-mode cation exchange sorbent (EVOLUTE® EXPRESS CX) was developed and various sample sizes were assessed to determine the optimal sample volume for a 98-compound DOA panel. A method involving microelution off of the sorbent requiring no evaporation or reconstitution steps were also developed.
Part No: AN009-HOR.V.1Issued year: 2015File size: 0.58mbFile type: pdf
Method 525.2 describes the procedure to determine a full suite of low concentration semi-volatile organic compounds in drinking water using solid phase extraction (SPE) or liquid–solid extraction (LSE) techniques. The City of Fort Worth, Water Department implemented an automated SPE process for the analysis of semi-volatiles by EPA Method 525.2 using the Atlantic® C18 solid phase extraction disk. Ethyl acetate, methanol and water were used to condition the Atlantic C18 disk prior to the extraction step. The extraction solvents used were a 1:1 mixture of methylene chloride and ethyl acetate. Extracts were then analyzed by GC/MS using a splitless injection technique.
Part No: AN108-HORIssued year: 2016File size: 2.35mbFile type: pdf
Drinking water is a critical resource important for human health. As water becomes more scarce, quality will become increasingly important and monitoring will be required more frequently and at lower concentration levels. The number of compounds and lower concentrations for monitoring will make extraction and analysis even more challenging.
Part No: AN019-HORIssued year: 2010File size: 0.92mbFile type: pdf
The City of Fort Worth, Water Department conducted an evaluation of the Atlantic™ solid phase extraction (SPE) C18 disks using EPA Method 608. This application note describes the procedure to determine low ppb levels of organochlorine pesticides and PCBs in wastewater. This procedure used methylene chloride as the primary extraction solvent, followed by a hexane solvent exchange. Extracts were then analyzed by GC/ECD using a pressure pulse injection technique.
Part No: AN021-HORIssued year: 2009File size: 0.89mbFile type: pdf
Method 1657 describes the procedure to determine low ppb levels of organophosphorus pesticides in municipal and industrial wastewater.
The City of Fort Worth Water Department implemented Automated SPE for the analysis of organophosphorous pesticides by EPA Method 1657, using the Atlantic™ C18 solid phase extraction disk.
The Biotage® Horizon 4790 Automated Extraction System with Envision®
Platform controller, and the DryVap® Automated Drying
and Concentration System were used in this application note.
Part No: AN072-HORIssued year: 2012File size: 0.88mbFile type: pdf
Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous environmental contaminants, naturally occurring in coal, crude oil, gasoline, and their byproducts (e.g. coal tar or creosote). In addition, PAHs are formed in the incomplete combustion processes of all organic materials, such as wood or fossil fuels. Consequently, the EU water framework directive (WFD) lists in its annex X the whole group of PAHs as priority hazardous substances.
Part No: AN038-HORIssued year: 2009File size: 2.13mbFile type: pdf
This application note demontrates that the Biotage® fully automated extraction, drying and concentration systems used with the Atlantic™ HLB disk are capable of extracting PAH compounds from sea water.
Typical extraction times using the Biotage® Horizon 4790 range from 20 to 25 minutes while drying and concentration on the DryVap® with the DryDisk® took approximately 35 minutes.
Part No: AN055-HORIssued year: 2015File size: 1.36mbFile type: pdf
Polybrominated diphenyl ethers (PBDEs) are a worldwide contamination problem. Structurally similar to PCBs, these compounds are long-lived in the environment and can bio-accumulate throughout the food chain. The health hazards of these chemicals have attracted increasing scrutiny and, as such, a great deal of research and regulations have been implemented to manage and control them.
Part No: AN011-HORIssued year: 2010File size: 0.93mbFile type: pdf
The second unregulated contaminant monitoring regulation (UCMR2) program was developed to monitor US drinking water sources for currently unregulated compounds. EPA Method 527 is categorized under List 1; Assessment Monitoring in the UCMR2 program. It focuses on a wide range of semi volatile organic contaminants including pesticides that were deferred during the first UCMR, flame retardants, and pyrethroid pesticides. This application for EPA Method 527 employs SPE with analysis by gas chromatography / mass spectrometry (GC/MS).
Part No: AN035-HORIssued year: 2009File size: 0.83mbFile type: pdf
The purpose of this application note is to demonstrate the use of a fully automated solid phase extraction (SPE) and concentration system that provides fast extraction while improving the quality and consistency of results for EPA Method 8270D.
Part No: AN104-HORIssued year: 2016File size: 1.48mbFile type: pdf
Drinking water is an important resource that can provide significant exposure to humans if it is polluted. Analysis of source water, which may come from surface water, groundwater or treated water must be evaluated for possible contaminants to ensure regulatory compliance.1 Drinking water at the tap is often evaluated for contaminants that may be introduced through leaks in the pipes or pipe materials.
Part No: AN036-HORIssued year: 2009File size: 0.86mbFile type: pdf
The purpose of this application note is to demonstrate the capabilities of the Biotage® Horizon 4790 Automated Extractor System when used for the analysis of nitramine, nitroaromatic, and nitrate ester compounds in surface and ground water.
Part No: AN058-HORIssued year: 2010File size: 0.9mbFile type: pdf
The purpose of this application note is to demonstrate the capabilities of the automated Horizon Technology solution when used for the analysis of EPA method 8061A phthalate ester compounds in surface and ground water. Method 8061A states gas chromatography/mass spectrometry (GC/MS) can be used as an alternative for compound confirmation for phthalate esters.
Part No: AN064-HORIssued year: 2015File size: 1.69mbFile type: pdf
Although not frequently a problem, recent incidents at sea have led to large amounts of crude oil being released and dispersed throughout the Gulf of Mexico, such as in the Deepwater Horizon Gulf oil spill of 2010. Originally presumed to be Louisiana Sweet Crude, sample testing later revealed a harsher form of crude containing a high amount of asphaltenes was actually being released.
Part No: AN063-HOR.V.1Issued year: 2012File size: 0.22mbFile type: pdf
Oftentimes it becomes necessary to test sea water for oil and grease analytes. Whether it is due to a controlled release of effluent water from a waste water treatment plant (usually during large rain storms) or a large oil spill, there is a need generated for a reliable, automated extraction and evaporation system.
Part No: AN061-HORIssued year: 2010File size: 0.74mbFile type: pdf
Throughout the world, a lack of regulation has been blamed for the detection of pharmaceuticals and personal care products (PPCP) in some bodies of water. While the detected amounts are usually relatively small, the persistence of these compounds means that they can cause untold damage to the ecosystem for years to come.
Part No: AN065-HORIssued year: 2010File size: 2.07mbFile type: pdf
Although not frequently a problem, recent incidents at sea have led to large amounts of crude oil being released and dispersed throughout the Gulf of Mexico. Originally presumed to be Louisiana Sweet Crude, sample testing later revealed a harsher form of crude containing a high amount of asphaltenes was actually being released. The differences are substantial, as Louisiana Sweet degrades more readily in nature than crude oil containing asphaltenes.