Part No: AN-IST1009SIssued year: 2008File size: 0.04mbFile type: pdf
This method describes the use of a strong cation exchange SPE column for the extraction of pyridoxine, riboflavin and thiamine from biological fluid samples. This represents recommendations for SPE method development. The proposed steps are based on experience with similar analytes and matrices, but have not been verified in Biotage laboratories. Please refer to section below for the analyte and matrix considerations that were made in developing this method. As for all method development, this procedure should first be developed using pure solvent spiked with analyte. Only after the chemistry is established should spiked matrix samples be tested.
Part No: T63Issued year: 2010File size: 0.07mbFile type: pdf
When not in use, the check valve ball can stick, preventing the elution of solvents into the
collection vessel. Prior to beginning an extraction, free the Check Valve by using the Check
Valve Tool (PN: 02-0725 for the 1000/3000XL Extractors, or PN: 02-0727 for the 4790
Part No: T38Issued year: 2009File size: 0.06mbFile type: pdf
The Bleed Valve Assembly is provided to control the level of vacuum applied to the Extractor. This
Tech Tip provides instructions on installing the Bleed Valve Assembly on the Horizon Technology
Part No: PPS347Issued year: 2014File size: 0.49mbFile type: pdf
Trends on chemical synthesis have changed over recent years, with a more targeted approach to molecular design becoming more prevalent. As a result, the speed with which a new compound can be synthesized is key to an efficient laboratory.
Part No: F0051403_01Issued year: 2014File size: 0.14mbFile type: pdf
The Speed-Vap IV is a modern evaporation system providing safe and simultaneous unattended evaporation of up to nine pans of evaporating solvent for extractable hydrocarbons such as hexane oil and grease extractions generated using methods such as US EPA 1664, ISO 11349:2010 or Standard Methods 5520. It can also be used to evaporate solvents from extracted soil hydrocarbons and fats from foods.
Part No: TN522Issued year: 2004File size: 0.03mbFile type: pdf
Microwave assisted organic synthesis has become an important tool to medicinal chemists for rapid organic synthesis. Thousands of research papers have appeared over the last decades on the application of microwave technology in organic synthesis.1 Some of the major advantages include a spectacular decrease in reaction time, improved conversions, clean product formation and wide scope for the development of new reaction conditions.
Part No: P215Issued year: 2020File size: 0.34mbFile type: pdf
Peptides are well suited for use as tool compounds to interrogate biological systems and processes due to their chameleonic ability to mimic a potential protein partner within the biological system of interest. Their use in this application continues to grow as the rules governing permeability across the plasma membrane are further elucidated. Unfortunately, a well-defined secondary structure is often required for productive interaction with the other protein partners within the system of interest. ACS, Spring, 2020
Part No: Issued year: 2002File size: 0.36mbFile type: pdf
The first decision that needs to be made is what the final analysis will be for the analyte. This will have an impact on the sample and cartridge size, as well as the final elution solvent. Gas chromatography offers higher sensitivity than HPLC, while HPLC is better suited for ionisable species and very high molecular weights. If LC-MS is available, minimal sample clean-up may be required.
Part No: P210Issued year: 2019File size: 0.56mbFile type: pdf
This poster evaluates two “pass through” solid phase extraction techniques: (protein removal/phospholipid depletion (ISOLUTE PLD+) and dual mode extraction (ISOLUTE HYDRO DME+)) and supported liquid extraction (ISOLUTE SLE+) for a group of about 100 drugs of abuse compounds in whole blood.
Part No: P167Issued year: 2017File size: 0.42mbFile type: pdf
For most organic and natural product chemists flash
chromatography is a necessary part of their research. As such, many chemists need quick isolation of at least one desired component from a crude mixture in relatively high yield and purity. This need for speed, purity, and yield pits these desires against each other as you can typically optimize on only two of the three goals.
In this poster, we will describe some techniques that help chemists optimize flash purification and maximize speed, yield, and purity.
Part No: P188Issued year: 2018File size: 0.33mbFile type: pdf
Herein we identified an optimized protocol
for removing an Acm protecting group using the Biotage®
Initiator+ Alstra™ and applied the protocol for a fully
automated synthesis with on-resin disulfide bond formation,
simplified with the Branches™ software feature. The results
presented herein provide a route amenable to the synthesis of
other disulfide rich peptides, greatly reducing the effort put
toward synthesizing these complex molecules.
Part No: P021Issued year: 2008File size: 1.87mbFile type: pdf
It is well known that traditional liquid-liquid extraction (LLE) provides very clean extracts prior to LC/MS analysis. Supported liquid extraction is analogous to traditional LLE, however, analyte partitioning takes place using an inert support material, rather than two immiscible liquids. This provides excellent extraction efficiencies while alleviating
many of the tedious liquid handling issues associated with LLE.
Part No: AN897Issued year: 2018File size: 2.27mbFile type: pdf
This application note describes a streamlined procedure for
sample pre-treatment and extraction of a panel of 49 drugs
of abuse from human hair, using Biotage® Lysera for matrix
micropulverization, prior to direct transfer to clean up using
ISOLUTE® SLE supported liquid extraction.
Part No: AN069-HORIssued year: 2011File size: 0.79mbFile type: pdf
Laboratories which take in a high volume of aqueous samples will know that every sample is different. Samples can contain varying amounts of suspended particulates and/or sediment due to either the source of the water, or the collection technique. Still other samples may form precipitates under elevated pH conditions resulting in
emulsions. These types of samples have historically proven very challenging when using solid phase extraction (SPE).
Part No: UI298.v1Issued year: 2019File size: 0.37mbFile type: pdf
1) Remember to add stir bar for efficient mixing. Catalysts, salts, or visible precipitate should be washed clear of head space and into solution. Particles adhering to glass head space could cause excessive heating increasing possibility of failure of vial. Stay within specified vial volume range (see diagram for proper filling).
Part No: TechTipIssued year: 2011File size: 0.04mbFile type: pdf
Within each disk holder there is a support screen. The disk holders that we will cover are used with a broad range of Horizon products. The placement and condition of the support screen is critical to the functioning of these products.
Part No: P080Issued year: 2014File size: 0.88mbFile type: pdf
This poster describes the benefits of supported liquid extraction and highlights its use in removal of endogenous matrix components that cause ion suppression/enhancement (matrix effects) in LC-MS/MS analyses.
Part No: P118Issued year: 2015File size: 0.48mbFile type: pdf
This poster examines the use of ISOLUTE SLE+ columns as an alternative to SPE for the extraction and clean up of hair samples containing drugs of abuse. SLE was found to be a simple, faster alternative to SPE for this type of analysis.
Part No: PPS560Issued year: 2019File size: 1.6mbFile type: pdf
ISOLUTE® SLE+ products contain Biotage second generation extraction media – a refined, porous, highly purified diatomaceous earth based sorbent – advanced support enabling chemists to mimic the liquid-liquid extraction mechanism, without chemically interacting with the sample.
Part No: P089Issued year: 2014File size: 0.79mbFile type: pdf
This poster presents a novel method for the simultaneous extraction, derivatization and subsequent detection of both the traditional 25-hydroxy and the biologically active 1α,25-dihydroxy vitamin D metabolites in serum.
Part No: Issued year: 2013File size: 0.35mbFile type: pdf
Leu-Enkephalin-amide (YGGFLNH2, Ca. MW = 554.65) was synthesized on the Rink amide ChemMatrix® resin at a scale of 0.5 mmol. Crude peptide (100 mg) was purified in duplicate using the Isolera Dalton equipped with a Biotage® SNAP KP-C18-HS 12g cartridge.
Part No: AN097Issued year: 2014File size: 0.73mbFile type: pdf
A branched peptidoglycan mimic and a tetra-branched antimicrobial peptide analogue were synthesized on a lysine scaffold using Biotage® Initiator+ Alstra™ microwave peptide synthesizer. These peptide modifications are challenging to synthesize and automate, however, the procedure was operationally simplified using Branches™.
Part No: AN053Issued year: 2010File size: 0.92mbFile type: pdf
It has recently been demonstrated that specific recognition of rhizobial bacteria by the signaling molecule Nod-factor receptor 5 (NFR5) relies on LysM domains. The LysM (lysine motif) domain is believed to be involved in the regulation of the interaction between plants and rhizobial bacteria to promote plant growth. The LysM domain is predicted to consist of two-α
helices and a two-stranded anti-parallel β-sheet in a β-α-α-β structure and has been identified in NFR5 by sequence alignment of the crystal structure with the LysM domain of Bacillus subtilis ykuD.2 The synthesis of the C-terminal and the N-terminal regions of LysM domains provide significant challenges, this is presumably due to the formation of β-sheet like structures, which are known to pose problems for peptide chain assembly.
Part No: AN055Issued year: 2012File size: 0.28mbFile type: pdf
C-terminal peptide aldehydes are key components in oxime and hydrazine ligations and their synthesis is therefore very desirable. A well established method for the synthesis of C-terminal modified peptides including peptide aldehydes, is the backbone amide linker (BAL) strategy (Scheme 1).1 In this strategy, the first amino acid is anchored by reductive amination followed by acylation of the newly formed secondary amine. Thus, the growing peptide chain is anchored not through the C-terminal carboxyl but through a backbone amide nitrogen giving access to, in principle, any C-terminal modification.
Part No: AN054Issued year: 2011File size: 0.39mbFile type: pdf
A number of biologically active natural products contain N-methyl amino acids. N-Methyl amino acid containing peptides are potentially useful therapeutics as they have improved pharmacological properties such as such as proteolytic stability, bioavailability, lipophilicity, enhanced potency and receptor selectivity. The synthetic challenges associated with the synthesis of peptides containing consecutive N-methyl amino acids are well known1,2 and often require coupling reagents such as PyBOP3, HATU4 or even triphosgene4 to obtain high coupling yields. Syro Wave.
Part No: AN052Issued year: 2010File size: 0.26mbFile type: pdf
One of the current challenges in peptide science is the assembly of long peptides and small proteins, which has to overcome the accumulation of side-reactions and the hurdles posed by so-called difficult sequences. During the synthesis of difficult sequences the peptide chain most likely becomes partially inaccessible typically due to the formation of secondary structures, especially β-sheets, which can inhibit acylation and deprotection during synthesis, resulting in truncated sequences. In addition, steric hindrance from β-branched amino acids can be a problem.
Part No: Issued year: 2014File size: 1.01mbFile type: pdf
We have demonstrated the capability of the Biotage® Initiator+ Alstra
microwave peptide synthesizer to fully synthesize branched and cyclic
peptides. The synthesis included specialized reactions of non-linear peptides and a high degree of purity was achieved. Furthermore, the Branches software feature provides an extensive overview for the scheduling and visualization of operations in order to make complex peptide modifications and is a great addition to the toolbox for the peptide chemist. Presented at EPS, Sofia, 2014.
Part No: Issued year: 2005File size: 0.29mbFile type: pdf
Microwave technology at ChemDiv
Standard procedure for Suzuki coupling:
Building block 1-25 (0.25 mmol), the correspondent boronic acid (0.50 mmol) was heated under microwave irradiation (180oC, 10-40 min) in the presence of catalyst [PdCl2(PPh3)2 or dichlorobis(triphenyl-phosphine)palladium(II) polymer bound and Cs2CO3 as a base in DME/Water (50/50) as solvent.