Part No: P064Issued year: 2014File size: 0.47mbFile type: pdf
This poster presents a simple method for the extraction and subsequent detection of both the traditional hydroxy metabolites and the biologically active dihydroxy metabolites in serum. High analyte recoveries and low ion suppression were demonstrated, allowing limits of quantitation at low pg/mL levels for the di-OH metabolites and < 1 ng/mL levels for the OH metabolites.
Part No: P156Issued year: 2017File size: 0.23mbFile type: pdf
Most drugs are excreted in urine as glucuronide conjugates. Hydrolysis using a beta-glucuronidase enzyme to convert the metabolites to their “free” form for analysis increases sensitivity. Red abalone (Kura Biotech), abalone (Campbell Scientific), and recombinant (IMCSzyme) beta-glucuronidase enzymes were evaluated to determine which provided the most complete hydrolysis of glucuronide metabolites without effecting the overall recovery of non-conjugated compounds.
EVOLUTE EXPRESS CX 96-well plates were used to extract hydrolysed urine samples, and the impact of th enzymes was compared.
MSACL 2017, Palm Springs
SOFT 2017, Boca Raton
Part No: TN-31.0712Issued year: 2012File size: 0.93mbFile type: pdf
EXPRESS represents a leap forward in high
throughput solid phase extraction. The EVOLUTE
EXPRESS range of 96-well SPE plates combines
powerful EVOLUTE sorbent chemistry with innovative
features that enhance productivity by optimizing
or even eliminating the need for some traditional
steps in the SPE procedure. By truly eliminating the
need for conditioning and equilibration, samples
can be prepared by loading the plate, washing away
interferences and eluting with compatible solvents
EVOLUTE® EXPRESS Solid Phase Extraction products
from Biotage are designed for all your sample
preparation challenges. Whether you are working in the field of bio-analysis during drug development, clinical or forensic
toxicology, or with the diversity of samples in food
safety and environmental applications, the tools and
methods used for sample preparation have a clear
impact on productivity. This user guide contains information about choosing the correct EVOLUTE EXPRESS product for your application and optimizing your methodology for best results.
Part No: P200Issued year: 2019File size: 2.44mbFile type: pdf
Sample preparation is generally regarded as a pain point for many labs as it can be laborious, time consuming and relatively expensive. In a high throughput setting performing offline chemistry also leads to increases in sample turnaround time and reporting.
This poster evaluates simplified sample preparation workflows using optimized sample hold-up technology allowing chemistry in-situ. Reduced labour, extraction complexity, processing time, solvent usage and resultant instrument contamination and downtime can offset upfront costs associated with sample preparation.
Part No: TN-0012.0610Issued year: 2010File size: 0.38mbFile type: pdf
Through innovative patented technology molecularly
imprinted polymers (MIPs) can afford improved clean-up in
your processes. Unwanted contaminants or high value
desirables can be efficiently extracted from processes
streams, resulting in more efficient production and cleaner
Part No: AN717Issued year: 2010File size: 0.48mbFile type: pdf
Pharmaceutical companies and those who supply them with intermediates, constantly strive to produce API’s and
intermediates of the highest purity. At the same time, many established production facilities face strong competitive
pressures to produce high quality product at lower costs. Drug substance impurities are wide-ranging as described in the
International Conference on Harmonization (ICH) “Impurities in new Drug Substances”1. Of particular concern in recent
years are the ‘genotoxic compounds.
Part No: P128v2Issued year: 2016File size: 0.4mbFile type: pdf
This poster discusses various stages of the sample preparation process to determine and assess the potential for cross contamination and present approaches to minimize and or eliminate the effect.
Part No: AN085-HORIssued year: 2013File size: 2.07mbFile type: pdf
The data given in this Application Note illustrates the fact that the combination of the Biotage(R) Horizon SmartPrep Automated Extractor, DryVap Concentrator, and DryDisk Separation Membranes represent a complete solution to the preparation of samples for the extraction of semi-volatile organics by EPA Method 525.3.
Part No: AN107-HORIssued year: 2016File size: 1.59mbFile type: pdf
China has set ambitious goals for cleanup and monitoring of drinking water. Methods that are rugged and sensitive are required to support this effort. Chinese Environmental Agency method SL 392-2007 has been developed for this purpose and specifies a C18 cartridge for extraction and GC/MS for the detection step.
Part No: AN715Issued year: 2011File size: 0.13mbFile type: pdf
This method demonstrates the extraction of a PAH standard test mix comprising of fluoranthene, benzo(a)anthracene, chrysene, benzo(b)fluoranthene, benzo(k)fluoranthene, benzo(a)pyrene, indeno(123-cd)pyrene, dibenzo(ah)anthracene, and benzo(ghi)perylene.
MIP, AFFINILUTE, SupelMIP, BIOTAGE, PAH, Polyaromatic Hydrocarbons