Part No: AN703Issued year: 2011File size: 0.04mbFile type: pdf
ISOLUTE EPH SPE columns and associated methodologies have been optimised to efficiently fractionate EPHs into aliphatic and polycyclic aromatic (PAH) fractions. The principle is similar to the approach taken by the Massachusetts Department of Environmental Protection (MADEP) and TPH criteria working group (TPHCWG) methods. However, compared to these methods, the ISOLUTE EPH fractionation column has been significantly reduced in size and the sorbent has been optimised in order to reduce solvent volumes and provide an automation compatible fractionation column. Automation of the fractionation process has been achieved without carryover of PAHs into the aliphatic fraction.
Environmental contamination, ISOLUTE, PAH, Polyaromatic hydrocarbons, soil,
Part No: P163Issued year: 2017File size: 0.58mbFile type: pdf
Over the past decade, the need for non-invasive drug screening that that precludes sample adulteration has become attractive. As a result, detection using oral fluid devices for Drugs of Abuse (DOA) has come to the vanguard of the scientific community. The use of Supported Liquid Extraction (ISOLUTE® SLE+) prior to LC/MS or GC/MS can improve sample cleanliness without forfeiting sample detection within a diverse panel of DOAs. Here, we demonstrate the effects of altering elution solvent polarity and pH for sample pretreatment upon the simultaneous recovery of 34 compounds comprised of opioids, benzodiazepines, and stimulants to directly measure the effects of the oral fluid buffer, OraSure™, upon extraction and signal intensity at presumed LOQs.
Part No: AN100Issued year: 2015File size: 0.16mbFile type: pdf
Biotage® Endeavor™ catalyst screening system was used to investigate the asymmetric hydrogenation of 4-aryl coumarins to 4-aryl-3,4-dihydrocoumarin, an important intermediate in the synthesis of endothelin receptor antagonists.
Part No: PPS512Issued year: 2019File size: 1.04mbFile type: pdf
Atlantic® ReadyDisks are solid phase extraction (SPE)
disks, pre-assembled into disposable holders and are
used for extracting organic and semivolatile organic
compounds from aqueous matrices. The ReadyDisks
are designed for single-use extractions with maximum
speed and convenience in mind and eliminate the
need to clean holders between extractions.
Part No: TN-0006.0506Issued year: 2006File size: 0.1mbFile type: pdf
The Atlantis pressure scale-up workstation performs and optimizes gas/liq and gas/liq/solid, heterogeneous reactions with the unique ability to monitor live gas consumption. Process
Chemists can easily load experiments with push-button hardware and precisely control and monitor reactions with simple-to-use software. The Atlantis system controls reaction parameters
automatically to eliminate sample degradation and in addition, provides concise reaction data reports with live molar uptake rates for better analysis.
Part No: AN045-HORIssued year: 2009File size: 0.65mbFile type: pdf
In this application note, a suite of neutral and list 1 pesticides in methylene chloride is evaporated using the Biotage® DryVap.
Pesticides containing many different functional groups, including sulfur and phosphorus groups, halides, nitramines, atrazines, alcohol, and carbonyl groups were efficiently concentrated for GCMS analysis.
This data indicates that the DryVap System is inert and effectively manages solvent evaporation without generating localized hot spots in the system.
Part No: AN893Issued year: 2018File size: 0.91mbFile type: pdf
This application note describes the fully automated extraction of carboxy-THC from urine, following base hydrolysis prior to GC/MS analysis. The method was automated using Biotage® ExtraheraTM, configured for use with EVOLUTE EXPRESS ABN columns.
The method delivers clean extracts and analyte recovery of 90% with RSD lower than 10% for carboxy-THC and its deuterated internal standard. Using the Biotage® ExtraheraTM, 24 samples were extracted in approximately 38 minutes. The limit of quantitation of 2 ng/mL is below the SAMHSA/ EWDTS confirmation cut off of 15 ng/mL for workplace testing applications.
Part No: AN892Issued year: 2018File size: 0.87mbFile type: pdf
This application note describes the fully automated extraction of carboxy-THC from urine, following base hydrolysis prior to GC/MS analysis. The method was automated using Biotage® ExtraheraTM, configured for use with ISOLUTE SLE+ columns.
The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 80% with RSDs lower than 10% for carboxy-THC and its deuterated internal standard. Using Biotage Extrahera, 24 samples are extracted in approximately 35 minutes. Limit of quantitation is below the SAMHSA/EWDTS confirmation cut off of 15 ng/mL for workplace testing applications.
Part No: P195Issued year: 2019File size: 0.8mbFile type: pdf
This poster shows an extraction protocol for 12 common drugs of abuse (DOA) to be detected in breast milk using mixed-mode polymeric cation exchange solid phase extraction (SPE).
Using the combination of reliable automation (on Biotage Extrahera) and SPE sample preparation techniques, a method was developed demonstrating the precision, accuracy, linearity, and sensitivity necessary for a robust quantitative workflow.
MSACL NA 2019
Part No: AN918Issued year: 2019File size: 1.33mbFile type: pdf
This application note describes the fully-automated extraction of opiates from acid-hydrolyzed urine and non-hydrolyzed urine prior to GC/MS analysis. The methods were automated using Biotage® Extrahera™ configured for use with ISOLUTE® SLE+ supported liquid extraction columns.
Using the Biotage® Extrahera™, 24 hydrolyzed samples were extracted in under 31 minutes and 24 non-hydrolyzed samples were extracted in under 36 minutes. The limits of quantitation meet or exceed the sensitivity requirements set by SAMHSA and EWDTS for workplace testing applications.
Part No: AN793Issued year: 2013File size: 2.04mbFile type: pdf
This application note describes the extraction of a range of SPICE drugs and metabolites in urine which are
typically screened in forensic toxicology panels using ISOLUTE® SLE+ in a 96-well plate format. Both manual
(Biotage Pressure+ 96) and automated (TECAN Freedom EVO® 100) processing conditions are described.
Part No: AN877Issued year: 2017File size: 2.61mbFile type: pdf
This application note describes the operating conditions for the automated fractionation of Extractable Petroleum hydrocarbons (EPH) into aliphatic and aromatic (PAH) fractions using the ISOLUTE EPH SPE column in conjunction with the Extrahera automation system.
Part No: AN094-HORIssued year: 2014File size: 1.41mbFile type: pdf
When a sample preparation technique calls for removal of compounds using SPE, it is standard practice to determine what concentrations will produce an acceptable linear range for the assay. Traditional linear regression is often performed manually using pipettes to generate an external standard curve that unknown samples are measured against.
Part No: Issued year: File size: 1.03mbFile type: pdf
• Solid-phase peptide synthesis (SPPS) is still often faced with challenges in the assembly of long and ‘difficult’ sequences, e.g. due to aggregation and steric hindrance giving rise to incomplete reactions. These problems have only partly been solved by new coupling reagents and solid supports.
• Precise microwave heating has emerged as one new parameter for SPPS, in addition to coupling
reagents, resins, solvents etc.
Part No: Issued year: File size: 0.13mbFile type: pdf
• Solid-phase peptide synthesis (SPPS) is still often
faced with challenges in the assembly of long and
‘difficult’ sequences, e.g. due to aggregation and
steric hindrance giving rise to incomplete reactions.
These problems have only partly been solved by
new coupling reagents and solid supports.
• Precise microwave heating has emerged as one
new parameter for SPPS, in addition to coupling
reagents, resins, solvents etc.
Part No: AN110-HORIssued year: 2016File size: 2.15mbFile type: pdf
This work demonstrates the performance of automated solid phase extraction (SPE) for the extraction of PAHs from surface water sampled from a nearby pond containing some particulate. An automated system (Biotage® Horizon 4790) with Atlantic® DVB disks, following the requirements of method EN 16691, was used.
Part No: AN109-HORIssued year: 2016File size: 1.5mbFile type: pdf
This application note describes the automated solid phase extraction (SPE) and GC/MS analysis of whole water samples according to EN16691 for PAHs.
Method EN 16691 is a recently developed method that uses solid phase extraction to isolate organic compounds from 1 L of water using a divinylbenzene (DVB) solid phase extraction disk.
Part No: AN122-HORIssued year: 2017File size: 1.87mbFile type: pdf
This application note demonstrates the performance of automated disk-based solid phase extraction (SPE), using Biotage® Horizon 5000 automated extraction system and Atlantic® DVB SPE disks, for concentration of organochlorine pesticides from whole water, prior to GC/MS analysis, following the requirements of EN16693:2015.
Part No: AN905Issued year: 2010File size: 0.25mbFile type: pdf
The extraction and segregation of Organic compounds from core samples taken from marine sediments is used to predict climate conditions, reconstruct sea temperatures and identify the type of vegetation from the C3 ( Temperate cli-mate) and C4 ( Tropical Climate) isotopic mass spectroscopy ratio analysis.
Part No: AN120-HORIssued year: 2017File size: 1.44mbFile type: pdf
Polycyclic aromatic hydrocarbons (PAHs) can be found in a number of water types, such as drinking water, surface water, ground water and wastewater. There are serval types of extraction techniques that can be implemented to remove them from water for measurement including liquid-liquid extraction (LLE), continuous liquid-liquid extraction (CLLE) and solid phase extraction (SPE). With LLE and CLLE there is little selectivity within the methods for difficult matrices or other compound interferences.
Part No: AN906.V.1Issued year: 2014File size: 1.29mbFile type: pdf
With the widespread clinical use of antidepressant drugs comes the need to accurately measure therapeutic lev-els to determine proper dosage, as well as check for toxic levels and patient compliance. More and more laboratories are shifting their therapeutic drug monitor-ing from liquid extraction to solid phase extraction. A large clinical laboratory has automated the process of extracting these drugs from serum using the RapidTrace SPE Workstation.
Part No: AN002-HORIssued year: 2015File size: 0.94mbFile type: pdf
This study shows that the Biotage® 4790 Automated Extractor system, along with the Atlantic™ DVB disk, the DryDisk® separation membrane, and the DryVap® Concentrator System can be successfully used to analyze for Nonyl Phenol and Bisphenyl A in water samples. This sample preparation process is fully automated, requiring minimal human intervention, and allowing for reductions in sample processing costs.
Part No: RP-DS-04Issued year: 2010File size: 0.19mbFile type: pdf
When analyzing human urine for drugs of abuse, one of the most common tests is for the cocaine metabolite Benzoylecgonine (BZE). A contract laboratory has automated this labor intensive procedure using the RapidTrace.
Part No: P101Issued year: 2014File size: 0.4mbFile type: pdf
Using the TLC to step gradient is simple and fast requiring only a minute or so to enter the data into the Isolera and create the method. The methods are scalable to larger cartridges increasing purification savings even more.
Part No: AN093.v2Issued year: 2014File size: 0.67mbFile type: pdf
We demonstrate the capability of the Biotage® Initiator+ Alstra™ microwave peptide synthesizer to fully automate the on-resin synthesis of cyclic peptides with examples showing disulfide bridge formation and side-chain to side-chain cyclization respectively.
Part No: P175Issued year: 2018File size: 2.14mbFile type: pdf
This poster presents a method for detection of approximately 100 drugs and metabolites in urine using mixed-mode polymeric cation exchange solid phase extraction (SPE).
Four different glucuronidase enzymes and several incubation times and temperatures were evaluated to determine optimum hydrolysis conditions for seven commonly detected glucuronide metabolites. Samples were subjected to offline hydrolysis and SPE (EVOLUTE® EXPRESS CX, Biotage, Charlotte, NC) and results compared to an SPE plate that incorporates in-well hydrolysis (EVOLUTE HYDRO CX, Biotage).
MSACL 2018, Palm Springs, CA
This brochure presents the extensive range of instrumentation for flash purification from Biotage. To complement these products Biotage also offers a complete range of flash consumables, including columns in a variety of sizes packed with irregular and spherical silica, making Biotage your one-stop partner for your flash purification needs.
Keywords: Isolera™ Dalton, Isolera™ Spektra, 10 Isolera™ Spektra One and Four, Isolera™ Spektra LS (Large Scale), Isolera™ Prime, Isolera™ ELSD-1080, Biotage® Flash 75/150, Biotage® Flash 400
Part No: BiotHR1Issued year: 2017File size: 0.15mbFile type: pdf
This statement sets out Biotage’s actions to understand all potential modern slavery risks related to its business and to put in place steps that aim at reducing the risk of slavery or human trafficking occurring within in its own business and its supply chains. This statement relates to actions and activities during the financial year 1 Jan 2016 to 31 December 2016.
Part No: PPS377.V.1Issued year: 2016File size: 1.13mbFile type: pdf
Biotage® Metal Scavenging Toolkit is an essential tool that can enable you to quickly and efficiently find the right metal scavenger for your application. It contains protocols, guidance and recommendations for working in batch mode or passing solutions through a fixed bed, this all in one kit showcases the metal scavengers and provides practical information on how to use the metal scavengers in your application for the best results.
Part No: PPS458Issued year: 2017File size: 1.04mbFile type: pdf
On Aug 30, 2017 a scientific literature search for the keywords Biotage AND peptide
was performed by Biotage personnel. The search identified 5380 peer-reviewed
publications from the global community, excluding patents and simple citations.
Below are approximately 100 titles illustrating the breadth and depth of research in
which Biotage tools are utilized.
Part No: PPS381Issued year: 2015File size: 1.07mbFile type: pdf
The versatility of Biotage® V-10 enables evaporation of single samples (up to 12 mL) or large pooled volumes using V-10 pumps (syringe or peristaltic). Adding the automated vial loading/changing carousel enables unattended evaporation of up to 16 samples.
Part No: Issued year: 2020File size: 108.97mbFile type: exe
Biotage® V-10 Touch Control Centre can be used to control any V-10 Touch system. The software is installed on an external computer and adds the capabilities of integrating the V-10 Touch system with a liquid handler as well as running the carousel with different methods for different carousel positions.