Part No: AN923Issued year: 2019File size: 3.7mbFile type: pdf
This application note describes the extraction of a panel of 19 steroid hormones from human urine using ISOLUTE® SLE+ Supported Liquid Extraction plates prior to LC/MS-MS analysis. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 90% with RSDs lower than 10% for all analytes. Linearity of greater than 0.999 is achieved for all analytes in the range 1-1000 pg/mL.
Manual and automated (using Biotage Extrahera) extraction protocols are detailed.
Part No: AN832Issued year: 2014File size: 1.78mbFile type: pdf
This application note describes the extraction of 47 drugs of abuse from oral fluid matrix after sampling via Quantisal collection devices prior to analysis by UPLC-MS/MS. Optimised protocols for 200 uL and 500 uL sample volumes are included.
Part No: AN836Issued year: 2015File size: 1.81mbFile type: pdf
This application note describes the extraction of 47 drugs of abuse and metabolites from oral fluid matrix collected using the Intercept Oral Fluid Drug Test Kit (Orasure Technologies), prior to UPLC-MS/MS analysis. The sample preparation is optimised to minimise matrix effects due to the buffers used in the collection device. Estimated LOQs range from 0.1-1 ng/mL for the various analytes.
Part No: AN837Issued year: 2015File size: 2.27mbFile type: pdf
This application note describes the extraction of 47 drugs
of abuse and metabolites from oral fluid matrix after sampling via Oral-Eze collection devices, prior to UPLC-MS/MS analysis using ISOLUTE SLE+ supported liquid extraction columns. Estimated LOQs of between 0.005 and 0.75 ng/mL (analyte dependant) are achieved,.
Part No: AN875Issued year: 2017File size: 3.36mbFile type: pdf
This application note describes the extraction, using ISOLUTE SLE+ supported liquid extraction columns, of 49 drugs of abuse from whole blood, prior to UPLC-MS/MS analysis.
High, reproducible drug recoveries are achieved, with sub ng/mL LOQs for most analytes. The method is easily automated using Biotage® Extrahera.
Part No: AN116-HORIssued year: 2017File size: 1.29mbFile type: pdf
Drinking water is a significant source of environmental exposure, especially for small children. Countries around the world have put regulations in place to monitor drinking water quality for a wide range of hazardous compounds. Methods such as SL 392-2007 in China, the EN methods in Europe and US methods such as method 525.2 cover a large suite of analytes of concern. They can be effectively extracted using solid phase extraction (SPE) disks and using GC/MS for detection.
Part No: AN114-HOR.V.1Issued year: 2009File size: 1.45mbFile type: pdf
US EPA method 525.2 may use solid phase extraction (SPE) to extract the analytes of interest from water samples. It includes a variety of quality control measures to ensure the method is under control throughout the analysis. The Biotage® Horizon 5000 was used in this study to extract the EPA Method 525.2 analytes from six prepared water samples.
The Atlantic high-capacity C18 disks provided excellent recovery of the large suite of compounds extracted in water. The Biotage® Horizon 5000 system provided uniform performance and a hands-off approach to the extraction step. The reproducibility of the six runs was excellent and the average of the standard deviation values was 2.57%.
Part No: AN830Issued year: 2014File size: 1.44mbFile type: pdf
This application note describes the use of the ISOLUTE® PLD+ Protein and Phospholipid Removal Plate for clean-up of a range of acidic, basic and neutral drugs from plasma. ISOLUTE® PLD+ Protein and Phospholipid Removal Plates are suitable for clean-up of a range of analytes with widely differing functionality and polarity characteristics from plasma, giving high recoveries, good reproducibility and excellent extract cleanliness.
Part No: AN775Issued year: 2012File size: 0.26mbFile type: pdf
This methodology has been designed to give an effective and efficient supported liquid extraction protocol for the clean-up and concentration of a range of forensically significant amphetamines followed by derivatization to optimize for GC-MS analysis. Analyte recoveries achieved using this method ranged from 99-104% with RSDs below 10% for all analytes.
Amphetamines, SLE, Supported Liquid Extraction, SLE+, ISOLUTE, Urine, GC, GC-MS,
Part No: AN758Issued year: 2012File size: 1.22mbFile type: pdf
Immunosuppressants inhibit or prevent the activity of the immune system used to prevent rejection of transplanted organs and treat autoimmune diseases. Due to the risks of immunodeficiency, those under treatment must undergo constant therapeutic drug monitoring (TDM) requiring reliable and robust analytical techniques for quantification of these drugs. Current methodologies use immunoassay techniques to measure immunosuppressant levels in patients which are expensive, time consuming and susceptible to issues with cross reactivity. The sample prep method in this application note offers an alternative approach to immunosuppressant analysis with LC-MS/MS giving more reliable and robust recoveries with no cross-reactivity and cost saving opportunities. Analyte recoveries range from 60-97% with RSDs all below 10%.
Immunosuppressant, Tacrolimus, Sacrolimus, Everolimus, Cyclosporin A, Clinical, SLE+, SLE, Supported Liquid Extraction, immune, Clinical, Whole Blood, immunoassay,
Part No: AN770Issued year: 2012File size: 0.26mbFile type: pdf
This application note demonstrates an effective and efficient suported liquid extraction protocol for the clean up and concentration of a range of forensically significant opiates and their metabolites
opiates, sle, urine, GC-MS, forensic, drugs of abuse, DOA, UCT, agilent
Part No: AN889Issued year: 2018File size: 1.03mbFile type: pdf
This application note describes a solid phase extraction protocol for the extraction of three catecholamine metabolites (vanillylmandelic acid, homovanillic acid and 5-hydroxyindoleacetic acid) from plasma prior to LC-MS/MS detection. The method described in this application achieves high reproducible recoveries and excellent linearity (>0.99) in the range 10-200 ng/mL.
Part No: AN888Issued year: 2017File size: 0.91mbFile type: pdf
This application note describes a supported liquid extraction protocol for the extraction of three acidic catecholamine metabolites (vanillylmandelic acid, homovanillic acid and 5-hydroxyindoleacetic acid) from plasma prior to LC-MS/MS detection. The method described in this application note achieves high reproducible recoveries (>80%, RSD < 5%) for these analytes in plasma with linearity > 0.995 in the range
2–200 ng/mL.
Part No: IST1069AIssued year: 2012File size: 0.11mbFile type: pdf
This application note demonstrates an effective and efficient suported liquid extraction protocol for the clean up and concentration of a range of forensically significant opiates and their metabolites
ISOLUTE, unknown screening,
Part No: IST1044AIssued year: 2011File size: 0.11mbFile type: pdf
The method addresses the extraction of all three classes of drugs of abuse from urine using non-polar and cation exchange retention mechanisms on the ISOLUTE HCX mixed mode column. This protocol was optimized to eliminate the use of dichloromethane in the elution of the acidic / neutral drug fraction.
Part No: P085Issued year: 2014File size: 0.46mbFile type: pdf
This poster demonstrates a novel, sensitive, cost effective and rugged method for the analysis of acrylamide in coffee and potato chips (crisps) using supported liquid extraction prior to LC-MS/MS
analysis. It demonstrates extraction from coffee down
to a concentration of 1 ng/mL and in potato chips to a level
of 10 ng/g (ppb).
ASMS 2014
Part No: AN796Issued year: 2013File size: 0.62mbFile type: pdf
This application note describes a Supported Liquid Extraction (SLE) protocol for the extraction of acrylamide from coffee using ISOLUTE® SLE+ columns with LC-MS/MS detection.
The method described in this application note achieves high recoveries of acrylamide in coffee. The method is sensitive enough to measure levels as low as 1 ng/mL in coffee (solution), 25 ppb in ground coffee (solid) or 125 ppb in instant coffee (solid, traditional or decaffeinated) and gives good selectivity from what is a challenging matrix.
Part No: IST1076AIssued year: 2011File size: 0.12mbFile type: pdf
This method describes the clean-up of acrylamide from cooked foodstuffs, using a multimode retention mechanism (hydrophobic, cation and anion exchange) for retention of interfering food components. The analytical technique is LC-MS-MS.
ISOLUTE, MULTIMODE, SPE, FOOD, FOOD SCARE
Part No: AN797Issued year: 2013File size: 0.94mbFile type: pdf
This application note describes a novel approach to the extraction of acrylamide from potato chips (crisps) using ISOLUTE SLE+ supported liquid extraction columns, with analysis by LC-MS/MS.
Part No: AN807Issued year: 2013File size: 1.05mbFile type: pdf
This application note describes an SPE protocol appropriate for LC-MS/MS analysis of aflatoxin M1 found in infant formula.
The method described in this application note achieves high recoveries of aflatoxin M1 from infant formula with %RSDs and LOQs that all meet the requirements set in European regulations for its measurement.