This application note describes a Supported Liquid Extraction (SLE) protocol for the extraction of nicotine, cotinine, 3-OH-cotinine, nornicotine, norcotinine and anabasine from urine, serum, plasma and whole blood using ISOLUTE® SLE+ plates with LC-MS/MS detection. nicotine cotinine nornicotine norcotinine 3-OH cotinine anabasine smoker

3-OH-cotinine Anabasine Application Notes Clinical Cotinine LC-MS/MS Nicotine Nicotine metabolites Norcotinine Nornicotine Plasma Serum Urine Whole blood

Molecularly imprinted polymers (MIPs) are a class of highly cross-linked polymers- engineered to bind one target compound or a class of structurally related target compounds with high selectivity. Selectivity is introduced during MIP synthesis in which a template molecule, designed to mimic the analyte, guides the formation of specific cavities or imprints that are sterically and chemically complementary to the target analyte(s). It is therefore critical for analysts to use the methodology described below when using this phase. Conventional generic methodologies employed with conventional SPE chemistries (e.g., reversed-phase C18) will yield sub-optimal results when employed with this phase.

Application Notes Column DMZOH Dimetridazole (DMZ) Food IPZOH Ipronidazole (IPZ) LC-MS/MS MNZOH Metronidazole (MNZ) Milk Nitroimidazoles Ronidazole Veterinary drugs Water

This Application Note highlights the use of supported-liquid Extraction to isolate three common NSAIDs, Sulindac, Flurbiprofen and Ibuprofen, from human plasma. Biotage, Ostro, Liquid liquid extraction, supported liquid extraction, Extrelute

Application Notes Clinical Flurbiprofen Ibuprofen LC-MS/MS NSAIDs Plasma Sulindac

Molecularly imprinted polymers (MIPs) are a class of highly cross-linked polymers- engineered to bind one target compound or a class of structurally related target compounds with high selectivity. Selectivity is introduced during MIP synthesis in which a template molecule, designed to mimic the analyte, guides the formation of specific cavities or imprints that are sterically and chemically complementary to the target analyte(s). It is therefore critical for analysts to use the methodology described below when using this phase. Conventional generic methodologies employed with conventional SPE chemistries (e.g., reversed-phase C18) will yield sub-optimal results when employed with this phase. molecularly imprinted polymers, MIP, BIOTAGE, AFFINILUTE, NSAIDs, Environmental, SupelMIP

Application Notes Clinical Clofibric Acid Column Ibuprofen LC-MS/MS NSAIDs Naproxen Post-Mortem fluids Post-Mortem tissue Water

This method describes the use of ISOLUTE SLE+ supported liquid extraction plates (96-well) and 1 mL sample volume columns to extract a range of opiates including opiate treatment analytes (methadone and its metabolite EDDP)) from human urine. This simplified and efficient extraction method has significant analyte recoveries ranging from 70-102% with LODs as low as 500 pg/mL. Opiates, SLE, SLE+, Supported Liquid Extraction, Urine, Oasis, Waters, SPE, Solid Phase Extraction, Sample Prep, quick, easy, DOA, Drugs of Abuse

6-acetylmorphine (MAM) Application Notes Codeine Column Dihydrocodeine (DHC) EDDP Forensic Hydrocodone Hydromorphone LC-MS/MS Methadone Morphine Opiates Oxycodone Oxymorphone Urine

This application note describes the extraction, using ISOLUTE SLE+ supported liquid extraction columns, of opiate compounds from oral fluid matrix collected using the NeoSal device, prior to GC/MS analysis.

6-acetylmorphine (MAM) Application Notes Codeine Column Dihydrocodeine (DHC) Drugs of Abuse English Forensic GC-MS Hydrocodone Hydromorphone Morphine Opiates Oral fluid Oxycodone Oxymorphone Saliva

This application note describes the extraction of opiate compounds from whole blood, prior to GC/MS analysis. This protocol also allows the simultaneous extraction of various other drugs of abuse classes: amphetamines, barbiturates, benzodiazepines and cocaine. ISOLUTE® SLE+ columns with 1 mL sample capacity are used to extract whole blood samples following a straightforward sample dilution. No protein precipitation or other pre-treatment is required prior to sample loading. The sample preparation procedure delivers clean extracts, good recoveries and RSD values and LLOQs from 50 ng/mL (analyte dependant).

6-acetylmorphine (MAM) Application Notes Clinical Codeine Column Dihydrocodeine (DHC) Drugs of Abuse English Forensic GC-MS Hydrocodone Hydromorphone Morphine Opiates Oxycodone Oxymorphone Whole blood

This application note was developed for the clean-up of a non-polar solvent extract of vegetable material. The methodology is applicable to a wide range of organochlorine and organophosphorus pesticides and a number of vegetable types. The analytes are relatively non-polar, and will not be strongly retained by polar/anion exchange sorbents.

Application Notes Clordane Column Dicofol Endosulfan Food Food GC-MS Organochlorines Organophosphate Pesticide Organophosphates Pesticides Vegetables

In this study, the effectiveness of the Atlantic™ DVB SPE Disk in extracting organochlorine pesticides from water samples was investigated, and an optimized method of extraction developed.

4,4,DDD Aldrin Alpha-Chlordane Application Notes DDD DDE DDT Dieldrin Disk EPA 8081 Analytes Endosulfan Endosulfan sulfate Endrin Endrin Aldehyde Endrin Ketone English Environmental Heptachlor Heptachlor epoxide Methoxychlor Organochlorine pesticides (OCP) US EPA Method 8081 Water α-BHC β-BHC γ-BHC δ-BHC

This method describes the use of EVOLUTE WAX plates and columns to extract a suite of organophosphate pesticide metabolites including dimethylphosphate (DMP), dimethylthiophosphate (DMTP), diethylphosphate (DEP), diethylthiophosphate (DETP), dimethyldithiophosphate (DMDTP) and diethyldithiophosphate (DEDTP) from human urine. The method demonstrates extraction of these small polar metabolites at urine concentrations ranging from 40 ng/mL to 400 ng/mL. resin, polymer, EVOLUTE,

Application Notes Biomarker Clinical Column Diethyldithiophosphate (DEDTP) Diethylphosphate (DEP) Diethylthiophosphate (DETP) Dimethyldithiophosphate (DMDTP) Dimethylphosphate (DMP) Dimethylthiophosphate (DMTP) LC-MS/MS Organophosphate Pesticide Urine

The environmental persistence of pharmaceuticals is of increasing interest, with little known regarding removal during wastewater treatment. This study investigates the potential of commercially available QuEChERS kits for the extraction and clean-up of a suite of common over-the-counter (OTC) pharmaceuticals from water and sludge cake. Extracts are analysed using LC-MS/MS.

Acetaminophen Analgesics Application Notes Cetrizine dihydrochloride Diclofenac Diphenhydramine hydrochloride English Environmental LC-MS/MS Over the counter pharmaceuticals QuEChERs Sludge cake Water

The method described in this application note achieves high reproducible extraction recoveries of the peptides oxytocin and vasopressin from serum using a fast, simple EVOLUTE® EXPRESS Load-Wash-Elute SPE procedure. Extraction from serum was performed using EVOLUTE EXPRESS ABN 96-well plates, utilizing a highly aqueous elution solvent, and minimizing co-extractable material in the form of proteins, lipids and phospholipids.

96-well plate Application Notes Clinical English LC-MS/MS Oxytocin Peptides Pharma Serum Vasopressin

The use of schedule I drugs for patient pain management therapy warrants constant monitoring of therapeutic levels in the patient. Screening patient urine samples for the free drugs is complicated by the metabolism process which converts the free drug to the -glucuronide form. Patient urine samples can be enzymatically hydrolyzed to cleave glucuronide moiety and produce the free form of drug. The target analytes can them be extracted from the urine matrix using EVOLUTE CX solid phase extraction cartridges. LOQs range from 5 ng/mL to 0.5 ng/mL with recoveries above 80% and RSD <10% Pain Management, EVOLUTE, CX, SPE, Solid Phase Extraction, Polymer, LC-MS-MS, Cocaine, Diazepam, Heroin, Morphine, Oxycodone, Fentanyl, Diazepam, Mixed Mode, RapidTrace

Alprazolam Application Notes Buprenorphine Butylone Clinical Clonazepam Codeine Column Fentanyl Flunitrazepam Hydrocodone Hydromorphone LC-MS/MS Meperidine Methadone Morphine Naloxone Naltrexone Oxycodone Oxymorphone RapidTrace Temazepam Urine

This application note describes a solid phase extraction (SPE) protocol for the extraction of patulin from clear apple juice using ISOLUTE Myco SPE columns, for analysis by LC-MS/MS. This method achieves high analyte recoveries with RSDs below 10%. mycotoxin, patulin, apple juice, LCMSMS

Apple juice (clear) Application Notes Column Food LC-MS/MS Mycotoxin Patulin

This application note describes the extraction of several peptides, ranging in size from a 7mer to a 54mer, from plasma and serum using EVOLUTE® ABN in a 96-well plate format. The presence of certain proteins and peptides in human biological matrices can be used as diagnostic markers for the onset of disease and other health problems in humans. The ability to detect and monitor lower levels of peptides can aid in clinical patient health assessments and therapeutic drug level determinations. The sample preparation methodology detailed in this application note can significantly increase sensitivity and detection of peptides from biological matrices on LC/MS/MS. This application note offers the user two different elution strategies due to the extensive combination of peptides that may need to be extracted either strategy may be suitable dependent upon which peptides are relevant. Peptides, EVOLUTE, SPE, Solid Phase Extraction, Proteins,

ACTH (1-24) Alpha-Endorphin Angiotensin Fragment 1-7 Angiotensin II, Application Notes Clinical Elafin Helicobacter pylori (2-20) LC-MS/MS Myelin Glycoprotein fragment (35-55) rat Peptides Plasma Vasoactive Intestinal Fragment 1-12

This procedure is recommended for the extraction of a variety of pharmaceuticals from water using non-polar polymeric SPE. These pharmaceuticals were identified as potential environmental contaminants by the Environment Agency. Pharmaceuticals, waste water, antibiotics, EVOLUTE,

Application Notes Atenolol Carbamazepine Citalopram Column Diclofenac Environmental Erythromycin Fluoxetine Fluvoxamine HPLC Ibuprofen Labetalol Mefenamic Metoprolol Oxprenolol Paroxetine Pharmaceuticals Propranolol Sotalol Sulfamethoxazole Trimethoprim Water

This application note describes the extraction of phencyclidine (PCP) from urine using supported liquid extraction and subsequent analysis by GC/MS.

Application Notes Column Drugs of Abuse English Forensic GC-MS Phencyclidine Phencyclidine (PCP) Urine

This method was developed for the extraction of phencyclidine from meconium for drugs ofabuse applications. The analyte has an ionisable amine group, which is exploited through cation exchange interactions. The pKa is approximately 8, so the compound is fully ionized at pH values of less than 6. The analyte also has significant non-polar characteristics, and both hydrophobic and ion exchange retention mechanisms are used to give a clean extract. ISOLUTE, PCP, DOA, Varian Bond Elut Certify, UCT CleanScreen DAU

Application Notes Clinical Column GC-MS Meconium Phencyclidine (PCP)

This application was developed for the extraction of phencyclidine and other basic drugs from urine for drugs of abuse applications. Typical recovery for PCP is > 85%. PCP, DOA, Biotage, ISOLUTE, UCT CleanScreen DAU, Varian Bond Elut Certify

Application Notes Column Forensic GC-MS Phencyclidine (PCP) Urine

Phosphatidylethanol is an alcohol biomarker with a high degree of specificity; blood concentration of PEth correlates to the amount of alcohol consumed. This application note describes the extraction of 3 common species of PEth from whole blood using ISOLUTE® SLE+ supported liquid extraction prior to HPLC-MS/MS analysis. The method is easily automated using the Biotage® Extrahera, and details of the automated procedure are included in the application note.

96-well plate Alcohol metabolite Application Notes Clinical English Extrahera Forensic LC-MS/MS PEth Whole blood