This method describes the clean-up of acrylamide from cooked foodstuffs, using a multimode retention mechanism (hydrophobic, cation and anion exchange) for retention of interfering food components. The analytical technique is LC-MS-MS. ISOLUTE, MULTIMODE, SPE, FOOD, FOOD SCARE

Acrylamide Application Notes Column Food Food Food contaminant LC-MS/MS

This application note describes a novel approach to the extraction of acrylamide from potato chips (crisps) using ISOLUTE SLE+ supported liquid extraction columns, with analysis by LC-MS/MS.

Acrylamide Application Notes Column English Food Food contaminant LC-MS/MS Potato chips (crisps)

This application note describes an SPE protocol appropriate for LC-MS/MS analysis of aflatoxin M1 found in infant formula. The method described in this application note achieves high recoveries of aflatoxin M1 from infant formula with %RSDs and LOQs that all meet the requirements set in European regulations for its measurement.

Aflatoxin M1 Application Notes Food Milk Mycotoxin

This application note describes a solid phase extraction(SPE) protocol for the extraction of a range of mycotoxins from dried chili (pimiento) using ISOLUTE® Myco with LC-MS/MS analysis. The method achieves high recoveries of aflatoxins and ochratoxin from dried chili (pimiento) with %RSDs and LOQs that meet the requirements set in European Union regulations for measurement of these analytes.

Aflatoxin B1 Aflatoxin B2 Aflatoxin G1 Aflatoxin G2 Application Notes Column Dried chili Food LC-MS/MS Mycotoxin Ochratoxin A Spice

This application note describes the extraction, using ISOLUTE SLE+ supported liquid extraction columns, of amphetamine style compounds from oral fluid matrix collected using the NeoSalTM device, prior to GC/MS analysis.

2C-B 5-APB 6-APB Amphetamine Amphetamines Application Notes BZP Butylone Column Designer stimulants Drugs of Abuse English Ethylone Forensic MDA MDEA MDMA MDPV Mephedrone Methamphetamine Methcathinone Methedrone Methylone Naphyrone Oral fluid Saliva TFMPP mCPP pMMA

This application note describes the extraction of a range of amphetamine-type compounds from whole blood, prior to GC/MS analysis. A protocol that allows the simultaneous extraction of various other drugs of abuse classes: barbiturates, benzodiazepines, cocaine and opiates, is also evaluated. ISOLUTE® SLE+ columns with 1 mL sample capacity are used to extract whole blood samples following a straightforward sample dilution. No protein precipitation or other pre-treatment is required prior to sample loading. The sample preparation procedure delivers clean extracts, good recoveries and RSD values and LLOQs from 10 ng/mL (analyte dependant).

2C-B 5-APB 6-APB Amphetamine Amphetamines Application Notes BZP Butylone Clinical Column English Ethylone Forensic GC-MS MDA MDEA MDMA MDPV Mephedrone Methamphetamine Methcathinone Methedrone Methylone Naphyrone TFMPP Whole blood mCPP pMMA

This method was developed for the extraction of amphetamine and methamphetaminefrom urine for drugs of abuse applications. Typical absolute recoveries for these drugs are > 80%. drugs of abuse

Amphetamine Application Notes Forensic GC-MS Methamphetamine Urine

This application note describes the extraction of amphetamine and amphetamine-style compounds from hair matrix prior to GC/MS analysis following bead homogenization.

Amphetamine Amphetamines Application Notes Bead Ruptor Clinical Column Drugs of Abuse English Forensic GC-MS Hair MDA MDEA MDMA Methamphetamine

This application note describes the extraction of a range of amphetamines and metabolites from urine using supported liquid extraction and subsequent analysis by GC/MS. Prior to extraction, a simple oxidation step is performed to eliminate sympathomimetic compounds such as ephedrine and pseudoephedrine so they do not interfere with quantitation of methamphetamine.

96-well plate Amphetamine Amphetamines Application Notes English Ephedrine Forensic GC-MS MDA MDEA MDMA Methamphetamine Pseudoephedrine Urine

This method describes the use of ISOLUTE SLE+ in 96 well plate format for the extraction of a range of amphetamines. This procedure is effective with sample volumes of 100 μL for analyte recoveries > 90%. For larger volume (500 μL) samples, see AN746. amphetamines, AN742, ISOLUTE, SLE+, DOA, DRUGS OF ABUSE, FORENSIC

Amphetamine Amphetamines Application Notes Ephedrine Forensic LC-MS/MS MDA MDEA MDMA Methamphetamine Urine

The following method(s) have been developed for the class-selective extraction of Amphetamine, Methamphetamine, Phentermine, MDA, MDMA, MDEA from human urine. The method is highly reproducible and offers an average recovery greater than 80%. MIP, AFFINILUTE, SupelMIP, BIOTAGE, Amphetamines,

Amphetamine Amphetamines Application Notes Forensic MDA MDEA Methamphetamine Phentermine Urine

This method describes the use of ISOLUTE SLE+ in column format for the extraction of a range of amphetamines. This method is effective for sample volumes of 500μL with analyte recoveries > 95%. Amphetamines, ISOLUTE, DOA, DRUGS OF ABUSE, SLE+, DRUG SCREEN

Amphetamine Amphetamines Application Notes Ephedrine Forensic LC-MS/MS MDA MDEA MDMA Methamphetamine Urine

This procedure is recommended for the extraction of extensive panel of significant therapeutic and illicit drugs of abuse from whole blood and urine using mixed-mode cation exchange SPE columns. ISOLUTE HCX is a silica based mixed-mode sorbent that combines non-polar and strong cation exchange retention mechanisms for extraction of basic drugs from biological fluids. Ion exchange, Drugs of Abuse, DOA, Clinical,

2-Hydroxyethylflurazepam 4-Hydroxynordiazepam 7-Aminoclonazepam 7-Aminoflunitrazepam 7-Aminonitrazepam Alprazolam Application Notes Benzodiazepines Bromazepam Chlordiazepoxide Clinical Clonazepam Clozapine Demethylflunitrazepam Desalkylflurazepam Diazepam English Estazolam Flunitrazepam Flurazepam Forensic Hydroxyalprazolam Hydroxymidazolam Hydroxytriazolam LC-MS/MS Midazolam Nitrazepam Nordiazepam Oxazepam Prazepam Temazepam Triazolam Urine Whole blood Zolpidem

Ethylestrenol and stanozolol are anabolic steroids which can be used to increase muscle mass and enhance performance. These drugs have been linked to instances of doping in race horses. This application note describes a Supported Liquid Extraction (SLE) protocol for the extraction of ethylestrenol and stanozolol from horse urine prior to LC-MS/MS analysis. The method achieves high reproducible analyte recoveries from both gelding and filly urine. Protocols for 48-well, 96-well plate and column formats are described.

48-well plate 96-well plate Anabolic steroids Application Notes Column English Ethylestrenol Forensic LC-MS/MS Stanozolol Urine (horse)

Antiepileptic drugs are prescribed to suppress seizures in epilepsy patients. A variety of different types of AEDs have been synthesized to pharmacologically address different types of epilepsy. The ability to therapeutically monitor these drugs in patients is necessary for maintaining optimal medical care and managing any adverse effects of thedrug. A fast and clean extraction method is needed that works in a variety of biological matrices and affords a high throughput workflow. In this poster, ISOLUTE SLE+ is demonstrated as an effective way to extract AEDs from serum, oral fluid and urine with good efficiency and recovery. The method was developed on a 96 well plate format to facilitate a high throughput workflow model. MSACL 2014

96-well plate Antiepileptics Carbamazepine 10,11 epoxide Clinical English Felbamate Gabapentin LC-MS/MS Oral fluid Oxcarbazepine Posters Rufinamide Saliva Serum Tigabatine Urine Vigabatrin

In this poster, ISOLUTE SLE+ is demonstrated as an effective way to extract antiepileptic drugs from serum, oral fluid and urine with good efficiency and recovery. The method was developed on a 96-well plate format to facilitate a high throughput workflow model. ASMS 2014

96-well plate Antiepileptics Carbamazepine 10,11 epoxide Clinical English Felbamate Gabapentin LC-MS/MS Oral fluid Oxcarbazepine Posters Rufinamide Saliva Serum Tigabatine Urine Vigabatrin

This application note describes the extraction of neutral and zwitterionic antiepileptic drugs from fortified oral fluid using ISOLUTE® SLE+ in a 96-well plate format.

96-well plate Antiepileptics Application Notes Carbamazepine 10,11 epoxide Clinical English Felbamate Gabapentin LC-MS/MS Oral fluid Oxcarbazepine Rufinamide Tigabatine Vigabatrin

The ability to therapeutically monitor antiepileptic drugs in patients is necessary for maintaining optimal medical care and managing any adverse effects of the drug. A fast and clean extraction method is needed that works in a variety of biological matrices and affords a high throughput workflow. Here ISOLUTE SLE+ is demonstrated as an effective way to extract AEDs from serum and urine with good efficiency. The method was developed using a 96-well plate format to facilitate a high throughput workflow model.

96-well plate Antiepileptics Application Notes Carbamazepine 10,11 epoxide Clinical English Felbamate Gabapentin LC-MS/MS Oxcarbazepine Rufinamide Serum Tigabatine Urine Vigabatrin

This method describes the use of ISOLUTE SLE+ supported liquid extraction 1 mL sample volume columns to extract a range of barbiturates from human urine. The analysis of these analytes was carried out by GC-MS. This simplified and efficient extraction method has significant analyte recoveries ranging from 103-108% with LOQs of 10 ng/mL and RSDs <10%. Barbs, Barbiturates, SLE, SLE+, ISOLUTE, GCMS, DoA, Forensic, Work Place Drug Testing, Supported Liquid Extraction, GC

Amobarbital Application Notes Barbiturates Butabarbital Butalbarbital GC-MS Phenobarbital Secobarbital Urine

The objective was to develop a GC-MS assay for the determination of barbiturates from urine matrix using Supported Liquid Extraction (SLE). The SLE extraction mechanism is very efficient, delivering higher analyte recoveries and cleaner extracts than equivalent LLE methods. Barbiturates, SLE, Supported Liquid Extraction, Forensic, SOFT, Urine,

Barbiturates Butabarbital Butalbarbital GC-MS Pentobarbital Posters Secobarbital Urine