Disinfection by-products (DBP) are an ever-present nuisance in the efforts to purify drinking water, wastewater and municipal waters from various sources. An emerging class of DBP compounds with health effects is nitrosamines1-3 which result from chloramination or chlorination if the water is nitrogen-rich.

Application Notes Drinking water EPA 521 Analytes English Environmental GC/MS/MS Nitrosamines Nitrosamines SmartPrep US EPA Method 521

In postmortem cases, where drugs or pesticides have been used for their poisonous properties, traditional matrices such as urine and whole blood may be inappropriate for qualitative and quantitative analysis. As the site of metabolism for most drugs and toxins, the liver may provide more insight to cause of death than other bodily fluids. This poster describes the use of ISOLUTE SLE+ supported liquid extraction columns to extract a range of drug and pesticide classes form homogenised liver using a simple, streamlined workflow. SOFT 2016

Alprazolam Amphetamine Atrazine Bead Ruptor Bendiocarb Biphenthrin Butabarbital Chlorothalonil Clinical Clonazepam Cocaine Column Diazepam Drugs of Abuse English Estazolam Forensic GC-MS Ketamine Liver MDMA Malathion Methadone Methamphetamine Midazolam Nitrazepam Pesticides Phenobarbital Posters Propanil Secobarbital THC Triazolam

This poster demonstrates the extraction of a range of drugs of abuse from oral fluid, collected with common collection devices, prior to UPLC-MS/MS analysis. The target analyte list includes benzodiazepines, z drugs, amphetamines, cathinones, opiates, cocaine, buprenorphine, PCP, THC-COOH, fentanyl and ketamine. SOFT 2014

11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH) 2-OH-Et-Flurazepam 6-acetylmorphine (MAM) 7-Aminoclonazepam 7-Aminoflunitrazepam Alprazolam Amphetamine Amphetamines Benzodiazepines Benzoylecgonine Bromazepam Buprenorphine Cathinones Clonazepam Cocaine Codeine Column Diazepam Dihydrocodeine (DHC) EDDP English Estazolam Fentanyl Flunitrazepam Flurazepam Forensic Hydrocodone Hydromorphone Ketamine LC-MS/MS Lorazepam MDA MDEA MDMA Mephedrone Methadone Methamphetamine Midazolam Morphine Nitrazepam Norbuprenorphine Nordiazepam Norfentanyl Norketamine Opiates Opiates Oral fluid Oxazepam Oxycodone Oxymorphone Phencyclidine (PCP) Posters Saliva Temazepam Triazolam Zaleplone Zolpidem Zopiclone z-drugs α-OH Triazolam α-OH alprazolam

This poster demonstrates the extraction of a range of drugs of abuse from oral fluid collection devices using supported liquid extraction suitable for UPLC-MS/MS analysis. Unlike some sample preparation techniques, SLE allows for the simultaneous extraction of cross-functional analytes in a single extraction protocol without forfeiting extract cleanliness. The target analyte list includes benzodiazepines, z drugs, amphetamines, cathinones, opiates, cocaine, buprenorphine, PCP, THC-COOH, fentanyl and ketamine. TIAFT 2015

2-OH-Et-Flurazepam 6-acetylmorphine (MAM) 7-Aminoclonazepam 7-Aminoflunitrazepam Alprazolam Amphetamine Amphetamines Benzodiazepines Benzoylecgonine Bromazepam Buprenorphine Cathinones Clonazepam Cocaine Cocaines Codeine Column Diazepam Dihydrocodeine (DHC) Drugs of Abuse EDDP English Estazolam Fentanyl Flunitrazepam Flurazepam Forensic Hydrocodone Hydromorphone LC-MS/MS Lorazepam MDA MDMA Mephedrone Methadone Midazolam Morphine Norbuprenorphine Nordiazepam Norfentanyl Norketamine Opiates Oral fluid Oxazepam Oxycodone Oxymorphone Pain Management Phencyclidine (PCP) Posters Temazepam Triazolam Zaleplone Zolpidem Zopiclone α-OH Triazolam α-OH alprazolam

This poster describes the extraction of a range of drugs of abuse (including barbiturates, THC and metabolites, benzodiazepines, z drugs, amphetamines,cathinones, opiates, cocaine, buprenorphine, PCP, fentanyl and ketamine) from oral fluid using supported liquid extraction (ISOLUTE SLE+) columns prior to GC-MS and LC-MS/MS analysis. ASMS 2014

11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH) 6-acetylmorphine (MAM) 7-Aminoclonazepam 7-Aminoflunitrazepam Alprazolam Amphetamine Amphetamines Barbiturates Benzodiazepines Benzoylecgonine Bromazepam Buprenorphine Clonazepam Cocaine Codeine Column Designer stimulants Diazepam Dihydrocodeine (DHC) EDDP English Estazolam Fentanyl Flunitrazepam Flurazepam Forensic GC-MS Hydrocodone Hydromorphone Hydroxyalprazolam Hydroxytriazolam Ketamine LC-MS/MS Lorazepam MDA MDEA MDMA Mephedrone Methadone Methamphetamine Midazolam Morphine Nitrazepam Norbuprenorphine Nordiazepam Norfentanyl Norketamine Oral fluid Oxazepam Oxycodone Oxymorphone Phencyclidine (PCP) Posters THC metabolites Temazepam Triazolam Zaleplone Zolpidem Zopiclone

This poster demonstrates a fast, reliable protocol to extract multiple drug of abuse panels from whole blood using a common supported liquid extraction methodology. This benefits laboratory workflow where multiple assays are run each day, saving both worker hours and consumable costs. SOFT 2015 ASMS 2016 TIAFT 2016

2-Hydroxyethylflurazepam 2C-B 5-APB 6-APB 6-acetylmorphine (MAM) 7-Aminoclonazepam 7-Aminoflunitrazepam Alprazolam Amobarbital Amphetamine Anhydroecgonine Methyl Ester AEME BZP Benzoylecgonine Bromazepam Butabarbital Butalbarbital Butylone Clinical Clonazepam Cocaethylene Cocaine Cocaines Codeine Column Diazepam Dihydrocodeine (DHC) Drugs of Abuse Ecgonine Methyl Ester EME English Estazolam Ethylone Flunitrazepam Flurazepam Forensic GC-MS Hexobarbital Hydrocodone Hydromorphone Lorazepam MDA MDEA MDMA MDPV Mephedrone Methamphetamine Methcathinone Methedrone Methylone Midazolam Morphine Naphyrone Nitrazepam Nordiazepam Oxazepam Oxycodone Oxymorphone Pentobarbital Phenobarbital Posters Secobarbital TFMPP Temazepam Triazolam Whole blood mCPP pMMA α-OH Triazolam α-OH alprazolam

Matrix components, particularly salts, proteins and phospholipids, can lead to ion suppression resulting in inaccurate quantitation and reduced detection limits. Resin-based mixed-mode cation exchange SPE sorbents are widely used for the extraction of basic compounds from biological fluids. The dual retention mechanism allows a two stage interference wash protocol, which results in extremely clean extracts. This poster will investigate the performance of EVOLUTE™ CX for the extraction of a wide range of basic drugs from plasma, showing high analyte recovery and advanced extract cleanliness. The analyte suite was selected to cover a variety of basic analytes with wide ranging polarities (LogP values). Extract cleanliness experiments were performed showing overall ion suppression, then individual matrix components examined in terms of protein and phospholipid removal.

Plasma Posters

This poster evaluates the extraction of a range of drugs of abuse from hydrolyzed and nonhydrolyzed urine using a novel flow through scavenging product, ISOLUTE® HYDRO DME+. Matrix component removal in terms of creatinine and urea, salt residue, pigmentation associated with urobillin content and protein removal will be demonstrated. SOFT 2018

96-well plate Barbiturates Benzodiazepines Clinical Cocaines Column Drugs of Abuse English Forensic LC-MS/MS Opiates Opioid Posters

This poster describes an evalution of Methylisothiazolinone (MI) extraction from sunscreen using supported liquid extraction prior to GC/MS analysis MSACL 2015, San Diego

Antimicrobial Clinical Column English Environmental GC-MS MI (methylisothiazolinone) Methylisothiazolinone Posters Sun Lotion Sunscreen

Methylisothiazolinone (MI) is used in a variety of personal care products, such as sunscreens, lotions, cosmetics. MI is a cytotoxin and as a result there is concern because of sensitization and allergic reactions as well as cell and nerve damage. A percentage of the population is at risk from contact dermatitis when exposed to this compound at sufficient concentrations. This poster describes the use of ISOLUTE SLE+ for extraction of MI from sunscreen. ASMS 2015

Antimicrobial Clinical Column Environmental GC-MS MI (methylisothiazolinone) Methylisothiazolinone Posters Preservative Sun Lotion Sunscreen

This poster compares the performance of manual processing to a novel automated sample preparation system prior to GC/MS or LC-MS/MS analysis in forensic toxicology applications. Emphasis is placed on the potential for 96-well cross contamination and strategies for its elimination. TIAFT 2016 SOFT 2016

2-Hydroxyethylflurazepam 2-OH-Et-Flurazepam 6-acetylmorphine (MAM) 7-Aminoclonazepam 7-Aminoflunitrazepam 96-well plate Alprazolam Amphetamine Amphetamines Benzodiazepines Bromazepam Buprenorphine Clinical Clonazepam Codeine Column Diazepam Dihydrocodeine (DHC) Drugs of Abuse EDDP English Estazolam Extrahera Fentanyl Flunitrazepam Flurazepam Forensic GC-MS Hydrocodone Hydromorphone Ketamine LC-MS/MS Lorazepam MDA MDEA MDMA Mephedrone Methadone Methamphetamine Midazolam Morphine Nitrazepam Norbuprenorphine Nordiazepam Norfentanyl Norketamine Opiates Opiates Oxazepam Oxycodone Oxymorphone Phencyclidine (PCP) Posters Temazepam Triazolam Urine Whole blood Zaleplone Zolpidem Zopiclone z-drugs α-OH Triazolam α-OH alprazolam

Matrix components, particularly salts, proteins and phospholipids, can lead to ion suppression resulting in inaccurate quantitation and reduced detection limits. Resin-based mixed-mode cation exchange SPE sorbents are widely used for the extraction of basic compounds from biological fluids. The dual retention mechanism allows a two stage interference wash protocol, which results in extremely clean extracts. This poster will investigate the performance of EVOLUTE™ CX for the extraction of a wide range of basic drugs from plasma, showing high analyte recovery and advanced extract cleanliness. The analyte suite was selected to cover a variety of basic analytes with wide ranging polarities (LogP values). Extract cleanliness experiments were performed showing overall ion suppression, then individual matrix components examined in terms of protein and phospholipid removal.

Basic drugs Biological fluids Posters

Amphetamine, methamphetamine and Ecstasy continue to be widely abused in many parts of the world. Urine analysis is the most popular approach to determining drug intake. This poster examines various sample preparation approaches in the analysis of amphetamines prior to gas chromatography-mass spectrometry. Comparisons were performed between silica-based and polymer-based SPE (ISOLUTE HCX and EVOLUTE EXPRESS CX) as well as supported liquid extraction (SLE, ISOLUTE SLE+). TIAFT 2019

Amphetamine Amphetamines Clinical English Forensic MDA MDEA MDMA Methamphetamine Posters Urine

Traditionally the analysis of aldosterone and angiotensin (for plasma renin activity measurement) are performed separately. However, the relationship of the aldosterone-to-renin-ratio is a very useful tool to help define the cause of secondary hypertension. This poster compares sample preparation options for the simultaneous extraction of Angiotensin I, II and Aldosterone from plasma. MSACL EU 2018 MSACL NA 2019

96-well plate Aldosterone Angiotensin I Angiotensin II, Clinical English Hormone Hormones LC-MS/MS Peptides Posters Steroid Steroid Hormones Steroids

For as long as column chromatography has been practiced by organic and medicinal chemists there has been a concern that too much methanol in the solvent system will dissolve the column’s silica. This concern has come about because chemists often find a solid white precipitate in collected fractions after purification. This phenomenon is usually noticed when dichloromethane and methanol are used as the mobile phase solvents. In this poster we will show the results of a study that evaluated the degree of dissolution for both granular and spherical silica in methanol and dichloromethane.

English Pharma Posters White Papers

This poster describes an improved workflow for the analysis of drugs of abuse from hair. TIAFT 2018, Ghent, Belgium

2-OH-Et-Flurazepam 6-acetylmorphine (MAM) 7-Aminoclonazepam 7-Aminoflunitrazepam 96-well plate Alprazolam Amphetamine Amphetamines Benzodiazepines Benzoylecgonine Bromazepam Buprenorphine Clonazepam Cocaine Codeine Column Diazepam Dihydrocodeine (DHC) EDDP English Estazolam Extrahera Fentanyl Flunitrazepam Flurazepam Forensic Hair Hydrocodone Hydromorphone Ketamine LC-MS/MS Lorazepam Lysera Lysergic acid diethylamide (LSD) MDA MDEA MDMA Methadone Methamphetamine Midazolam Morphine Nitrazepam Norbuprenorphine Nordiazepam Norfentanyl Norketamine Opiates Opioid Oxazepam Oxycodone Oxymorphone PCP Posters Temazepam Triazolam Zaleplone Zolpidem Zopiclone z-drugs α-OH Triazolam α-OH alprazolam

This poster describes an improved workflow for the analysis of drugs of abuse from hair. Implementation of bead homogenization along with automated sample preparation allowed for simplified methodology. The direct solvent extraction approach avoids the need for pre-concentration while maintaining desired LOQs with either 200 or 400 μL of hair extract. SOFT 2018

96-well plate Amphetamines Benzodiazepines Column English Extrahera Forensic Hair LC-MS/MS Lysera Opiates Opioid Posters

Hair analysis is growing in popularity due to the non-invasive nature of the sample collection. Although not used as routinely as other matrices such as blood or urine it does have advantages in that the matrix can indicate prolonged drug exposure. NCFM 2020, Reykjavik, Iceland

2-OH-Et-Flurazepam 6-MAM 7-amino-flunitrazepam 96-well plate Alprazolam Amphetamine Bromazepam Buprenorphine Cannabinoid Clonazepam Cocaine Codeine Diazepam Drugs of Abuse EDDP Estazolam Fentanyl Flunitrazepam Flurazepam Forensic Hair Hydrocodone Hydromorphone Ketamine Lorazepam Lysera Lysergic acid diethylamide (LSD) MDA MDEA MDMA Mephedrone Methadone Methamphetamine Midazolam Morphine Nitrazepam Norbuprenorphine Nordiazepam Norfentanyl Norketamine Oxazepam Oxycodone Oxymorphone PCP Posters THC THC-COOH Temazepam Triazolam UPLC-MS/MS Zaleplone

Sample preparation for hair analysis is often lengthy involving multiple manual labor steps from cutting, washing, homogenization/pulverization, digestion, sample extraction and analysis. This poster aims to demonstrate a streamlined sample preparation workflow for hair analysis. The workflow is evaluated using a suite of drugs of abuse, including THC and metabolites. MSACL NA 2019

6-MAM 7-Aminoclonazepam 7-Aminoflunitrazepam 96-well plate Amphetamine Amphetamines Benzodiazepines Bromazepam Clinical Clonazepam Codeine Drugs of Abuse EDDP English Flunitrazepam Flurazepam Forensic Hair Hydromorphone LC-MS/MS Lysera MDA MDEA MDMA Mephedrone Methadone Methamphetamine Midazolam Morphine Nitrazepam Opiates Opioid Oxycodone Oxymorphone Posters THC THC metabolites THC-COOH

This poster will demonstrate SPE sample preparation strategies for the simultaneous extraction of both ethyl glucuronide and ethyl sulphate from urine, allowing cleaner extraction protocols compared to traditional dilute and shoot approaches. EtG, EtS, Urine, Poster, SOFT, Forensic, UPLC, LC-MS/MS, EVOLUTE, AX, WAX

96-well plate Alcohol metabolite Column Ethyl Sulfate (EtS) Ethyl glucuronide (EtG) Forensic LC-MS/MS Posters Urine