This application note describes a simple supported liquid extraction method for extraction of estrone and estradiol from human serum. An analytical HPLC-MS/MS method that avoids the use of derivatization or fluoride adduct stabilization (two approaches often used to enhance method sensitivity) is used. High, reproducible analyte recovery and low detection limits (0.001 ng/mL) are achieved.

96-well plate Application Notes Clinical English Estradiol Estrogens Estrone LC-MS/MS Serum Steroid Hormones

Ethyl glucuronide (EtG, Figure 1.) is an ethanol metabolite formed by the glucuronidation of the parent molecule. The presence of EtG in urine and plasma is a useful and sensitive biomarker of alcohol intake in forensic toxicology. EtG can be detected at very low levels and is useful in distinguishing between ingested ethanol and that which is adsorbed through the skin from ethanol. This application note has been optimized on the RapidTrace+; an updated version of the current RapidTrace software which allows the system to process smaller bed heights than were previously possible. The application note shows analyte recoveries all above 97% with RSDs >10%. EVOLUTE, Resin, EtG, Alcohol, Alcohol Biomarker, metabolite, anion exchange, Strata X, Oasis, Waters,

Alcohol metabolite Application Notes Column Ethyl glucuronide (EtG) LC-MS/MS RapidTrace Urine

The following methods have been developed and optimized for the extraction of fluoroquinolones (FQL) from a variety of sample matrixes including bovine kidney, honey, and milk. Example FQLs include sarafloxacin, norfloxacin, enrofloxacin, ciprofloxacin, lomefloxacin, and ofloxacin.

Application Notes Bovine kidney Ciprofloxacin Column Enrofloxacin Fluoroquinolones Food Honey LC-MS/MS Milk Norfloxacin Sarafloxacin Veterinary drugs

The following methods have been developed for the selective extraction of the tobacco specific nitrosamine, NNAL (4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol) from aqueous sample matrices such as biological fluids (e.g., urine). The method is highly reproducible and offers NNAL recoveries in the range of 90% and a limit of detection below 5 ppt. AFFINILUTE, MIP, Molecularly Imprinted Polymers, SupelMIP

Application Notes Biological fluids Clinical Column LC-MS/MS NNAL Plasma Tobacco Specific Nitrosamines (TSNAs) Urine

This application note describes the extraction of the 'date rape' drug GHB (gamma-hydroxybutyric acid) from urine using supported liquid extraction and subsequent analysis by GC/MS. Both 96-well plate and column formats are included, and demonstrate good analyte recovery and reproducibility, at levels appropriate for determining illicit use of GHB.

96-well plate Anaesthetic Application Notes Column English Forensic GC-MS GHB Gamma hydroxybutyric acid Urine

This application note describes the extraction of a broad range of analytes from liver tissue matrix prior to GC/MS analysis using ISOLUTE® SLE+ supported liquid extraction columns. A protocol has been developed that allows the simultaneous extraction of various drugs of abuse classes: amphetamines, barbiturates, benzodiazepines, cocaine, ketamine, THC. In addition to these drug panels, simultaneous extraction of carbamate, organochlorine, organophosphate, pyrethroid, and triazine pesticide classes is demonstrated.

Alprazolam Amphetamine Application Notes Atrazine Bead Ruptor Bendiocarb Biphenthrin Butabarbital Chlorothalonil Clinical Clonazepam Cocaine Cocaines Column Diazepam Drugs of Abuse English Estazolam Forensic GC-MS Ketamine Liver MDMA Malathion Methadone Methamphetamine Midazolam Nitrazepam Pesticides Phenobarbital Propanil Secobarbital THC Triazolam

This application note describes the extraction of a range of illicit drugs, which are typically screened in forensic toxicology panels, from oral fluid using ISOLUTE® SLE+ supported liquid extraction columns. oral fluid, Quantisal, Immunalysis, Intercept, Orasure, supported liquid extraction

6-acetyl codeine 6-acetylmorphine (MAM) Alprazolam Buprenorphine Clonazepam Codeine Column Diazepam EDDP English Fentanyl Flunitrazepam Forensic Hydrocodone Hydromorphone LC-MS/MS Morphine Naltrexone Nitrazepam Normeperidine Oral fluid Oxazepam Oxycodone Oxymorphone Temazepam

This application note describes the extraction of testosterone and androstenedione from female patient serum samples using ISOLUTE® SLE+ (96 well) plates. This method has been optimized to extract testosterone and androstenedione from female clinical serum samples. The method has achieved very low limits of quantitation for testosterone and androstenedione 0.4nmol/L and 0.9nmol/L respectively. The recoveries for both analytes were greater than 90%. Testosterone, Androstenedione, Clinical, Serum, ISOLUTE, SLE+, steroids,

Androstenedione Application Notes Clinical LC-MS/MS Serum Steroid Hormones Testosterone

This method was developed for the extraction of lysergic acid diethylamide (LSD) from whole blood using a mixed non-polar and cation exchange retention mechanism. LSD, BIOTAGE, ISOLUTE, HCX, DOA, Hallucinogen

Application Notes Column Forensic HPLC Hydromorphone Whole blood

Malachite green (MG) and crystal (gentian) violet (CV) are triphenylmethane dyes used in aquaculture as fungicides and ectoparasiticides. They are rapidly metabolised to the leucomalachite green (LMG) and leucocrystal (gentian) violet (LCV) metabolites that persist in fish tissue. MG and CV are banned in many countries due to their mutagenicity and carcinogenicity, but may still be used illegally. Monitoring methodologies must be able to detect the compounds at 1 ng/g (US) or 2 ng/g (EU). In order to meet these LODs for complex matrices such as oily fish, and the low UV absorbance of the leuco forms, LC-MS-MS is the analytical method of choice. This application note focuses on ease of use when compared to other methods, and describes a QuEChERS based approach incorporating the use of aluminium oxide in enhanced dispersive SPE to reduce the levels of fat in the final extract. Additional cleanup is provided by polymeric EVOLUTE SPE columns, and the resulting extract demonstrated low ion suppression. Analyte recoveries of 60-105%, with low rsds, are achieved. The method is also applicable to Brilliant green. Malachite green, Leuco malachite green, Crystal violet, Leuco crystal violet, Brilliant Green, EVOLUTE, EVOLUTE CX, QuEChERS, LC/MS/MS, Salmon, Contamination, Gentian violet, SPE, Solid Phase Extraction, Polymer, Resin,

Application Notes Brilliant Green Column Crystal Violet Fish Food Food Gentian Violet LC-MS/MS Leuco Crystal Violet Leuco Malachite Green Malachite Green Salmon Triphenylmethane Dyes Veterinary drugs

This application note describes the extraction of melamine from biological fluids using EVOLUTE CX. Sustained melamine exposure can result in kidney stones and renal failure, with the young being most susceptible. food scare, Melamine, China, kidney failure, renal failure, protein, Biotage, EVOLUTE,

Application Notes Biological fluids Column Food Food contaminant LC-MS/MS Melamine

This application note describes the extraction of melamine from biological fluids using EVOLUTE CX. Melamine's low cost and high nitrogen content has led to exploitation in various sections of the food industry, most notably involving dairy products. Biotage, cation exchange, oasis, food scare, china,

Application Notes Column Food Food contaminant LC-MS/MS Melamine Milk

Melamine is traditionally used in making plastics, however, it’s low cost and high nitrogen content has led to exploitation in various sections of the food industry, most notably involving dairy products. Sustained melamine exposure can result in kidney stones and renal failure, with the young being most susceptible. This application note has been optimized on the RapidTrace+; an updated version of the current RapidTrace software which allows the system to process smaller bed heights than were previously possible. The application note shows recoveries of 88-90% with RSDs <10%. Melamine, food scare, china, EVOLUTE, cation exchange, RapidTrace, Automation, SPE, sample prep,

Application Notes Column Food Food contaminant LC-MS/MS Melamine Milk RapidTrace

This method demonstrates the extraction of Mephedrone (MCAT) from a range of biological fluids including plasma, urine and whole blood. This extraction is achieved through cation exchange using EVOLUTE CX, a mixed-mode resin-based SPE sorbent. Analytes were quantified using LC-MS/MS and recoveries were all above 90%. SPE, ION EXCHANGE, EVOLUTE, CX, STRATA X, OASIS, DOA

Application Notes Biological fluids Forensic LC-MS/MS Mephedrone Plasma Urine Whole blood

The screening for elevated levels of Methylmalonic Acid (MMA) in serum is commonly used as a clinical diagnostic indicator of Cobalamin (Vitamin B12) deficiency in mammals. The significance of this screening is important for the detection of inborn metabolic disorders that can lead to the build up MMA in human biological systems

96-well plate Acidic Biomarker Column English LC-MS/MS Methylmalonic acid (MMA) Posters Serum

This application note describes a simple, effective protocol for the extraction of methylmalonic acid (MMA) from serum using EVOLUTE® EXPRESS AX solid phase extraction plates. Samples are prepared using the EVOLUTE EXPRESS load-wash-elute procedure. The method can be easily automated using the Biotage Extrahera. Details of the automated procedure and data comparing manual and automated method performance are included.

96-well plate Acidic Application Notes Biomarker Clinical English Extrahera LC-MS/MS MMA (Methylmalonic acid) Methylmalonic acid (MMA) Serum

This application note describes a simple, effective ISOLUTE® PLD+ protocol for the extraction of methylmalonic acid (MMA) from serum, demonstrating high, reproducible analyte recoveries with low protein and phospholipid content in the extracts. The method can be easily automated using the Biotage Extrahera. Details of the automated procedure and data comparing manual and automated method performance are included.

96-well plate Acidic Application Notes Biomarker Clinical English Extrahera LC-MS/MS MMA (Methylmalonic acid) Methylmalonic acid (MMA) Serum

This application note describes a simple ISOLUTE® PPT+ protocol for the extraction of methylmalonic acid (MMA) from serum, demonstrating high, reproducible analyte recoveries with low protein content in the extracts. ISOLUTE PPT+ Protein Precipitation plates offer a fast and extremely simple sample preparation approach. Serum proteins are efficiently removed from the sample using an in-plate, ‘solvent first’, protein crash and filter procedure, which is easily automated using the Biotage Extrahera system. Details of the automated procedure and data comparing manual and automated method performance are included.

96-well plate Acidic Application Notes Biomarker Clinical English Extrahera MMA (Methylmalonic acid) Methylmalonic acid (MMA) Serum

This application note describes a simple, effective protocol for the extraction of methylmalonic acid (MMA) from serum using ISOLUTE® SAX strong anion exchange solid phase extraction plates, demonstrating high, reproducible analyte recoveries with low protein and phospholipid content in the extracts. Very clean extracts are possible due to the purely anion exchange retention mechanism available using this silica based sorbent. This minimizes the co-extraction of endogenous interferences through hydrophobic (or non-polar) retention. The method can be easily automated using the Biotage Extrahera. Details of the automated procedure and data comparing manual and automated method performance are included.

96-well plate Acidic Application Notes Biomarker Clinical English Extrahera LC-MS/MS MMA (Methylmalonic acid) Methylmalonic acid (MMA) Serum

This application note describes the extraction of methylmalonic acid from serum, prior to LC-MS/MS analysis. An effective and efficient ISOLUTE® SLE+ protocol optimized for the 200 μL capacity 96-well plate format is used. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 80% with RSDs below 10%. The method can be easily automated using the Biotage Extrahera. Details of the automated procedure and data comparing manual and automated method performance are included.

96-well plate Acidic Application Notes Biomarker Clinical English Extrahera LC-MS/MS MMA (Methylmalonic acid) Methylmalonic acid (MMA) Serum