Part No: P128v2Issued year: 2016File size: 0.4mbFile type: pdf
This poster discusses various stages of the sample preparation process to determine and assess the potential for cross contamination and present approaches to minimize and or eliminate the effect.
Part No: AN085-HORIssued year: 2013File size: 2.07mbFile type: pdf
The data given in this Application Note illustrates the fact that the combination of the Biotage(R) Horizon SmartPrep Automated Extractor, DryVap Concentrator, and DryDisk Separation Membranes represent a complete solution to the preparation of samples for the extraction of semi-volatile organics by EPA Method 525.3.
Part No: AN107-HORIssued year: 2016File size: 1.59mbFile type: pdf
China has set ambitious goals for cleanup and monitoring of drinking water. Methods that are rugged and sensitive are required to support this effort. Chinese Environmental Agency method SL 392-2007 has been developed for this purpose and specifies a C18 cartridge for extraction and GC/MS for the detection step.
Part No: AN715Issued year: 2011File size: 0.13mbFile type: pdf
This method demonstrates the extraction of a PAH standard test mix comprising of fluoranthene, benzo(a)anthracene, chrysene, benzo(b)fluoranthene, benzo(k)fluoranthene, benzo(a)pyrene, indeno(123-cd)pyrene, dibenzo(ah)anthracene, and benzo(ghi)perylene.
MIP, AFFINILUTE, SupelMIP, BIOTAGE, PAH, Polyaromatic Hydrocarbons
Part No: P209Issued year: 2019File size: 1.25mbFile type: pdf
this poster compares different extraction techniques (mixed-mode SPE: ISOLUTE HCX and EVOLUTE EXPRESS CX; Dual mode extraction (ISOLUTE HYDRO DME+) and supported liquid extraction (ISOLUTE SLE+)) for extraction of fentanyl and fentanyl analogues from both urine and whole blood.
Part No: AN833Issued year: 2015File size: 1.33mbFile type: pdf
This application note describes a polymer based-based weak exchange mixed-mode SPE protocol for the extraction of the aminoglycoside antibiotics gentamicin, streptomycin, spectinomycin and neomycin from chicken meat prior to LC-MS/MS analysis. Linearity and LOQ meet USDA-FSIS guidelines.
Part No: P120Issued year: 2015File size: 0.63mbFile type: pdf
This poster describes a comparison of 25-hydroxy Vitamin D Extraction using Supported Liquid Extraction and Phospholipid Depletion Plate Technology using Manual and Automated Sample Preparation prior to LC/MS Analysis. It was presented at MSACL 2015, San Diego
Part No: AN778Issued year: 2013File size: 0.88mbFile type: pdf
This methodology has been designed to give an effective and efficient supported liquid extraction protocol for the clean-up and concentration of salivary cortisol levels. Cortisol is a steroid hormone that when measured from saliva can be used as an indication of stress. Analyte recoveries achieved using this method ranged from 96-99% with RSDs below 3% (n=7) for all analytes.
Cortisol, Saliva, SLE, Supported Liquid Extraction, ISOLUTE, Clinical, Steroid Hormones, Stress
Part No: AN777Issued year: 2013File size: 0.86mbFile type: pdf
Cortisol is a steroid hormone that in urine can be used to diagnose hyper or hypo cortisol diseases such as Cushing’s Syndrome. This methodology has been designed to give an effective and efficient supported liquid extraction protocol for the clean-up and concentration of urinary cortisol levels. Analyte recoveries achieved using this method ranged from 99-101% with RSDs below 5% for all analytes.
Cortisol, Steroid hormone, Cushing's Syndrome, Cushing's Disease, Clinical, SLE, Supported Liquid Extraction, Stress,
Part No: AN841Issued year: 2015File size: 0.82mbFile type: pdf
This application note describes the extraction of 11-nor-9-carboxy-THC from a urine matrix, prior to GC/MS analysis.
Carboxy-THC is the primary metabolite of THC, a key indicator of illicit marijuana usage. In urine, ~80% of the carboxy-THC metabolite is present in the form of its glucuronide metabolite. Therefore, to effectively quantify the THC-COOH, urine is hydrolyzed before extraction. This application note describes optimized extraction of urine samples prepared by either enzymatic or base hydrolysis.
Part No: AN720Issued year: 2011File size: 0.22mbFile type: pdf
This application note describes the extraction of THC and metabolites from urine using ISOLUTE SLE+ columns or plates. The most prevalent marker in biological samples taken from cannabis abusers is 11-nor-9-carboxy-Δ9-THC. Here we demonstrate a supported liquid extraction procedure for 11-nor-9-carboxy-Δ9-THC.
Part No: P050Issued year: 2013File size: 0.46mbFile type: pdf
The use of schedule I drugs for patient pain management therapy warrants constant monitoring of therapeutic levels in patient urine samples. Screening patient urine samples is further complicated by the metabolic process which converts the free drug to the - glucuronide form. The target drugs in patient urine samples can be enzymatically hydrolysed and extracted using Supported Liquid Extraction (ISOLUTE SLE+) which offers an efficient alternative to traditional liquid-liquid extraction (LLE) and solid phase extraction (SPE) techniques typically used for this type of clinical sample preparation.
San Diego, MSACL, 2013
Part No: AN764Issued year: 2012File size: 0.52mbFile type: pdf
The use of schedule I drugs for patient pain management therapy warrants constant monitoring of therapeutic levels in the patient. Screening patient urine samples for the free drugs is complicated by the metabolism process which converts the free drug to the -glucuronide form. Patient urine samples can be enzymatically hydrolyzed and extracted to detect the drugs using Supported Liquid Extraction (ISOLUTE SLE+) which offers an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation. LOQs for recovered drugs ranged from 10 ng/mL to 0.5 ng/mL with recoveries above 80% and RSDs <10%
SLE, Supported Liquid Extraction, Pain Mangement, Hydrolysis, Urine, Buprenorphine, Oxycodone, Temazepam,