Part No: AN803Issued year: 2013File size: 1.28mbFile type: pdf
This application note describes effective and efficient protocols optimized for loading volumes of either 400 μL or 1 mL using ISOLUTE SLE+ supported liquid extraction columns. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 85% with RSDs of <10% for all analytes.
Part No: AN802Issued year: 2013File size: 1.19mbFile type: pdf
This application note describes effective and efficient protocols optimized for sample volumes of either 400 μL or 1 mL using ISOLUTE SLE+ supported liquid extraction columns. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 80% with RSDs of <10% for all analytes.
Part No: AN856Issued year: 2015File size: 0.7mbFile type: pdf
This application note describes the extraction of cocaine and major metabolites from whole blood, prior to GC/MS analysis. This protocol also allows the simultaneous extraction of various other drugs of abuse classes: amphetamines, barbiturates, benzodiazepines and opiates.
ISOLUTE® SLE+ columns with 1 mL sample capacity are used to extract whole blood samples following a straightforward sample dilution. No protein precipitation or other pre-treatment is required prior to sample loading. The sample preparation procedure delivers clean extracts, good recoveries and RSD values and LLOQs from 20 ng/mL (analyte dependant).
Part No: AN602Issued year: 2011File size: 0.23mbFile type: pdf
This Application Note highlights the use of ISOLUTE® SLE+ supported-liquid extraction plates for the extraction of corticosteroids from human plasma, specifically Triamcinolone, Prednisolone, Hydrocortisone, Prednisone, Betamethasone, Cortisone, Dexamethasone, Flumethasone, Corticosterone, Beclomethasone, Triamcinolone, Fluocinolone, Budesonide 1, Budesonide 2 and 5-Pregnen-3β−ol-20-one.
Part No: AN808Issued year: 2013File size: 1.2mbFile type: pdf
This application note describes the extraction of a range of recreational ecstasy-like drugs, designer stimulants and the hallucinogen 2C-B from urine prior to GC/MS analysis. The method described in this application note may also be suitable for extraction of other related analogs and derivatives of these drug compounds, for example the substituted cathinone class (bathsalts). This application note is optimized for extraction of 1 mL sample volumes.
Part No: AN887Issued year: 2017File size: 1.45mbFile type: pdf
This application note describes a solid phase extraction (SPE) protocol for the extraction of the peptide desmopressin from human serum prior to LC-MS/MS analysis.
The method uses EVOLUTE(R) EXPRESS WCX 96-well plates to achieve high recoveries with minimal matrix effects.
Part No: AN700.V.1Issued year: 2017File size: 0.9mbFile type: pdf
This procedure is recommended for the extraction of a variety of diuretics from urine using non-polar polymeric SPE. The analyte suite includes thiazides, carbonic anhydrase inhibitors, loop and potassium sparring diuretics, with wide ranging pKa and logP values.
Part No: AN930Issued year: 2020File size: 0.68mbFile type: pdf
This application note demonstrates recoveries of all the analytes within tables 1–2 of EPA method 625.1 (plus a few analytes from table 3) obtained using the Biotage® Horizon 5000 with Atlantic® 8270 One Pass solid phase extraction disks, 8270 Carbon Cartridges Max-Detect cartridges, and 1.0 Micron Atlantic® Fast Flow Pre-Filters for analyte extraction. The DryDisk® Solvent Drying System and the TurboVap® II are used for solvent drying and concentration and analysis is by GC-MS.
Part No: AN860.V.1Issued year: 2016File size: 0.89mbFile type: pdf
This application note describes a simple supported liquid extraction method for extraction of estrone and estradiol from human serum. An analytical HPLC-MS/MS method that avoids the use of derivatization or fluoride adduct stabilization (two approaches often used to enhance method sensitivity) is used.
High, reproducible analyte recovery and low detection limits (0.001 ng/mL) are achieved.
Part No: AN748Issued year: 2011File size: 0.23mbFile type: pdf
Ethyl glucuronide (EtG, Figure 1.) is an ethanol metabolite formed by the glucuronidation of the parent molecule. The presence of EtG in urine and plasma is a useful and sensitive biomarker of alcohol intake in forensic toxicology. EtG can be detected at very low levels and is useful in distinguishing between ingested ethanol and that which is adsorbed through the skin from ethanol. This application note has been optimized on the RapidTrace+; an updated version of the current RapidTrace software which allows the system to process smaller bed heights than were previously possible. The application note shows analyte recoveries all above 97% with RSDs >10%.
EVOLUTE, Resin, EtG, Alcohol, Alcohol Biomarker, metabolite, anion exchange, Strata X, Oasis, Waters,
Part No: AN945Issued year: 2020File size: 4.63mbFile type: pdf
This application note describes the extraction of a panel of fat soluble vitamins (including those representing Vitamins A, D, E & K) from human serum using ISOLUTE® SLE+ Supported Liquid Extraction plates prior to LC/MS analysis.
The simple sample preparation procedure delivers clean extracts and analyte recoveries approximately or above 90% with RSDs 10% or less for all analytes.
Part No: AN714Issued year: 2011File size: 0.1mbFile type: pdf
The following methods have been developed and optimized for the extraction of fluoroquinolones (FQL) from a variety of sample matrixes including bovine kidney, honey, and milk. Example FQLs include sarafloxacin, norfloxacin, enrofloxacin, ciprofloxacin, lomefloxacin, and ofloxacin.
Part No: AN713Issued year: 2011File size: 0.09mbFile type: pdf
The following methods have been developed for the selective extraction of the tobacco specific nitrosamine, NNAL (4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol) from aqueous sample matrices such as biological fluids (e.g., urine). The method is highly reproducible and offers NNAL recoveries in the range of 90% and a limit of detection below 5 ppt.
AFFINILUTE, MIP, Molecularly Imprinted Polymers, SupelMIP
Part No: AN828Issued year: 2014File size: 1.48mbFile type: pdf
This application note describes the extraction of the 'date rape' drug GHB (gamma-hydroxybutyric acid) from urine using supported liquid extraction and subsequent analysis by GC/MS.
Both 96-well plate and column formats are included, and demonstrate good analyte recovery and reproducibility, at levels appropriate for determining illicit use of GHB.
Part No: AN915Issued year: 2019File size: 1.53mbFile type: pdf
This application note describes the extraction of 25 illicit and prescribed drugs from hydrolyzed urine using ISOLUTE® HYDRO DME+ Dual Mode Extraction plates prior to UPLC-MS/MS analysis.
Because of the enhanced sample clean up delivered by ISOLUTE HYDRO DME+ products, analyte sensitivity is significantly increased compared to traditional dilute and shoot (D&S) techniques, resulting in reduced limits of quantitation (LOQ)
Part No: AN864Issued year: 2016File size: 2.43mbFile type: pdf
This application note describes the extraction of a broad range of analytes from liver tissue matrix prior to GC/MS analysis using ISOLUTE® SLE+ supported liquid extraction columns.
A protocol has been developed that allows the simultaneous
extraction of various drugs of abuse classes: amphetamines, barbiturates, benzodiazepines, cocaine, ketamine, THC.
In addition to these drug panels, simultaneous extraction of carbamate, organochlorine, organophosphate, pyrethroid, and triazine pesticide classes is demonstrated.
Part No: AN788Issued year: 2013File size: 1.57mbFile type: pdf
This application note describes the extraction of a range of illicit drugs, which are typically screened in forensic toxicology panels, from oral fluid using ISOLUTE® SLE+ supported liquid extraction columns.
oral fluid, Quantisal, Immunalysis, Intercept, Orasure, supported liquid extraction
Part No: AN762Issued year: 2012File size: 0.08mbFile type: pdf
This application note describes the extraction of testosterone and androstenedione from female patient serum samples using ISOLUTE® SLE+ (96 well) plates. This method has been optimized to extract testosterone and androstenedione from female clinical serum samples. The method has achieved very low limits of quantitation for testosterone and androstenedione 0.4nmol/L and 0.9nmol/L respectively. The recoveries for both analytes were greater than 90%.
Testosterone, Androstenedione, Clinical, Serum, ISOLUTE, SLE+, steroids,