Part No: AN881Issued year: 2017File size: 1.66mbFile type: pdf
The method described in this application note demonstrates selective extraction of the thyroid hormones T3, rT3 and T4 from serum. 200 μL of serum was extracted using the EVOLUTE® EXPRESS AX 30 mg fixed well plate format. High reproducible recoveries and low phospholipid content were observed, demonstrating limits of quantitation < 50 pg/mL.
Part No: AN884Issued year: 2017File size: 0.62mbFile type: pdf
TSNAs, or tobacco-specific nitrosamines, are carcinogens found in tobacco products, including e-cigarettes and smokeless tobacco. This proof of concept study focusses on NNN (n-nitrosonornicotine), but the supported liquid extraction methodology described is a also suitable for NNK (4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone) and NNAL (4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol).
Part No: AN716Issued year: 2011File size: 0.14mbFile type: pdf
The following method has been developed for the extraction of the following triazines and triazine metabolites; atrazine, simazine, propazine, cyanazine, sebuthylazine deisopropylatrazine, deethylatrazine, deethylterbutylazine, prometon and hydroxyterbutylazine from water. Recoveries are in the range 80-95% and are reproducible, allowing sharp decision limits. The maximum accepted level of triazines in drinking water is 0.1 μg/L (EU legislation).
AFFINILUTE, MIP, SupelMIP, Molecularly Imprinted Polymer
Part No: AN601Issued year: 2008File size: 0.12mbFile type: pdf
This Application Note describes the development of an automated procedure for high throughput supported-liquid extraction of three tricyclic antidepressant drugs from human plasma, using the ISOLUTE® SLE+ Supported-liquid Extraction Plate. Analyte recovery, along with the speed and efficiency are compared to traditional LLE.
Biotage, Extralute, Ostro, supported liquid extraction, liquid liquid extraction,
Part No: AN712Issued year: 2011File size: 0.09mbFile type: pdf
The following methods have been developed for the selective extraction of tobacco specific nitrosamines (NNN, NNK, NAT, and NAB) from human urine. The method is highly reproducible and offers an average recovery of 80% (95% for NNK and NAT; and 70% for NNN and NAB). Detection limits of 4 pg/mL are readily achieved for urine samples using LC-MS-MS analysis.
MIP, SupelMIP, Molecularly Imprinted Polymer,
Part No: P145Issued year: 2016File size: 0.42mbFile type: pdf
This poster demonstrates a rapid and reliable sample preparation method using Supported Liquid Extraction (ISOLUTE SLE+) to extract a suite of 30 hormone analytes from a hydrolyzed urine matrix. Single injection analysis by LC-MS/MS shows that matrix effects are eliminated by the ISOLUTE SLE+ protocol and that analyte recovery and sensitivity have excellent clinical utility.
Part No: AN880Issued year: 2017File size: 3.38mbFile type: pdf
The method described in this application note achieves high reproducible extraction recoveries of vitamin B7 from serum while minimizing co-extractable material in the form of proteins, lipids and phospholipids. Serum is extracted using the EVOLUTE® EXPRESS ABN 96-well plate.
Part No: AN757Issued year: 2012File size: 1.61mbFile type: pdf
Vitamin D deficiency can result in a variety of health issues such as osteoporosis, liver and kidney problems, it is often associated with increased risk of cancers and multiple sclerosis. From this standpoint vitamin D monitoring is on the rise and of significant clinical relevance. This method describes the use of ISOLUTE SLE+ plates for the efficient and simple extraction of the vitamin D metabolites 25-OH vitamin D2 and 25-OH vitamin D3. Incorporated into the procedure is an integral protein binding disruption step which maximizes analyte recovery and eliminates the need for any offline protein disruption. This method has been internally validated using DEQAS approved serum samples and deuterated internal standards the results obtained are better than that required for criteria for acceptable performance for
DEQAS approval. All recoveries were consistently greater than 90% with all RSDs < 10% at LOQs of 1-3.75 ng/mL.
Vitamin, Vitamin D, VitaminD, D, Clinical, SLE+, SLE, ISOLUTE, Metabolites, Supported Liquid Extraction, 25 Hydroxy Vitamin D,
Part No: AN706Issued year: 2011File size: 0.1mbFile type: pdf
The following methods have been developed for the selective extraction of beta-agonists from biological matrices. Methods have been developed for both biological tissues (e.g. bovine muscle) and fluids (e.g. urine). The methods are highly reproducible and offer β-agonist recoveries in the range of 35-90%.
MIP, Molecularly Imprinted Polymers, BIOTAGE, AFFINILUTE, SupelMIP,
Part No: Issued year: 2013File size: 0.52mbFile type: pdf
Developed in collaboration with the OC Crime Lab, this poster describes the extraction of 25 benzodiazepines and sedatives from various matrices using ISOLUTE SLE+ Supported Liquid Extraction columns. This poster was presented at SOFT 2013.
Part No: Issued year: 2010File size: 1.23mbFile type: pdf
User report: Initiator. Prof. Nakamura at the Gakushuin University has described the Initiator as an essential tool for his group’s cutting-edge research. We asked Prof. Nakamura for his views on the latest trends in the fields of organic synthesis and drug discovery.
Part No: P049Issued year: 2012File size: 0.27mbFile type: pdf
This poster outlines a new method for the extraction of a mixture of peptides from plasma using resin based SPE. Initial method optimization was performed using EVOLUTE ABN before comparison to the streamlined approach without plate conditioning and equilibration using EVOLUTE EXPRESS ABN. Presented at EBF 2012.
EBF, EBF 2012, Peptide, ABN, SPE, Polymer, Polymeric,
Part No: AN903 (2)Issued year: 2004File size: 0.14mbFile type: pdf
The rapid synthesis of the non-steroidal anti-inflammatory agent Fenclofenac (2-(2,4-dichlorophenoxy)phenyl acetic acid) under microwave irradiation is reported. Modified reaction conditions of traditional Ullmann ether coupling resulted in high yields of the diaryl ether. The Willgerodt–Kindler transformation and subsequent hydrolysis of thioamide resulted in 52%
overall yield of Fenclofenac.
Part No: AN016-HORIssued year: 2009File size: 1.28mbFile type: pdf
This application note uses the Biotage® Horizon 4790 automated extraction system with a DVB SPE disk to extract an aqueous sample for PAH compounds analysis. The extract is dried using the Horizon Technology DryDisk®.
Part No: AN806Issued year: 2013File size: 1.03mbFile type: pdf
The screening of patient urine samples for basic drugs is typically performed following a lengthy sample preparation methodology that requires a time consuming dry down step.
A novel elution protocol coupled to the EVOLUTE EXPRESS advanced plate technology affords a fast sample preparation solution that follows a LOAD-WASH-ELUTE-ANALYZE strategy. Basic drugs are fortified into urine and extracted in 3 easy steps using EVOLUTE EXPRESS CX solid phase extraction in a 96-well plate format.
Part No: AN794Issued year: 2013File size: 1.01mbFile type: pdf
This application note describes a method of extraction for metanephrine and normetanephrine from pooled
plasma using EVOLUTE® EXPRESS WCX 96-well SPE plates.
A new solid phase extraction
technology has been developed to reduce the time
associated with sample preparation methods by eliminating
traditional steps in the extraction workflow. Leveraging this
technology, a method protocol was developed using a load,
wash and elute approach. This method was optimized for