The results from this research show that the chromatographic purification of organic amine compounds can be improved simply by using an amine-functionalized silica and typical normal-phase solvents such as hexane and ethyl acetate.

Posters

Biotage has developed tools for every step of the organic process, with the entire workflow in mind. This dedicated suite of products vastly expands the range of options in order to truly accelerate discoveries of new molecules for future innovations.

Brochures

US EPA method 550.1 is a method used by regulatory agencies to determine PAH’s in drinking water by liquid-solid extraction (LSE) and high performance liquid chromatography (HPLC).1 The method outlines the steps needed to perform extractions of PAHs from drinking water sources and finished drinking water; as well as the quality control (QC) program requirements to ensure the method is operated under control. Use of Biotage automated sample preparation products allows users to achieve the precise and consistent data needed to comply with EPA method 550.1 QC specifications as stated in method, section 10.3.2., for Initial Demonstration of Capability (IDC); while at the same time, streamlining laboratory practices to increase lab productivity.

4790 Acenaphthene Acenaphthylene Acenapthalene Anthracene Application Notes Benzo (a) Anthracene Benzo (a) Pyrene Benzo (g,h,i) perylene Benzo (g,h,i) perylene Benzo (k) Fluoranthene Benzo(a)anthracene Benzo(a)pyrene Benzo(b)fluoranthene Chrysene Dibenz (a,h) Anthracene Disk Drinking water EPA 550.1 Analytes English Environmental Fluoranthene Fluorene HPLC Indeno (1,2,3-cd) Pyrene Napthalene Phenanthrene Polyaromatic Hydrocarbons (PAH) Polyaromatic hydrocarbons Pyrene

PAL-ChemMatrix is highly recommended for microwave synthesis. Under microwave heating conditions, such as Biotage Initiator the PAL-ChemMatrix provides higher purity peptides than the Rink Amide ChemMatrix. For manual synthesis or non-microwave synthesis, we recommend using the Rink Amide ChemMatrix resin.

Product Notes

A series of eight 3-alkylidylindolin-2-ones were conveniently synthesized on Biotage® Endeavor™ in high yield and purity. Reactor purging, agitation, pressurization, heating, gas uptake and run termination were performed automatically based on the run program.

Application Notes

How to uninstall Java 7 and install Java 6 to get Pathfinder working

Software

Application Notes

This work demonstrates that peptide purification efficiency by flash chromatography is improved with Biotage® SNAP Bio cartridges containing a spherical wide pore stationary under a wide variety of purification conditions.

Application Notes

Biotage has developed a range of tools to improve the peptide synthesis workflow, from synthesis of crude peptides through to the final purified product. These solutions make your workflow more efficient and improve the quality of peptides you synthesize. Our workflow solutions are used in pharma, biotech, CRO and academic laboratories throughout the world.

Brochures

Dr. Andrew Jamieson leads a team of peptide chemists at the University of Glasgow, where they synthesize complex molecules for chemical biology applications. An important tool that contributes to their success has been Biotage® Initiator+ Alstra™ microwave peptide synthesizer, and they are even breaking the dogma that only HPLC can be used for peptide purification, by using cutting edge reversed phase flash purification techniques provided by Biotage.

Customer Cases

Biotage ZIP flash chromatography cartridges are engineered to meet the needs of chemists requiring high performance and consistency from a purification cartridge at a value price to reduce purification costs. Isolera Prime flash purification system.

Product Notes

Application Notes

Application Notes

Application Notes

Sample preparation is an essential technique prior to LC-MS/MS analysis of drugs in biological fluid samples to remove unwanted matrix components. These matrix components, particularly salts, proteins and phospholipids, can lead to ion suppression resulting in inaccurate quantitation and reduced detection limits. Resin-based mixed-mode cation exchange SPE sorbents are widely used for the extraction of basic compounds from biological fluids. The dual retention mechanism allows a two stage interference wash protocol, which results in extremely clean extracts. This poster investigates the performance of EVOLUTE™ CX for overall ion suppression, then individual matrix components examined in terms of protein and phospholipid removal.

Posters

PS-HOBt(HL) is a higher loading version (0.9 – 1.4 mmol/g) of a sulfonamide-linked, resin-bound equivalent of 1-hydroxybenzotriazole (HOBt). PS-HOBt(HL) can be used as an effective activating reagent for transfer of protecting groups, e.g., Fmoc and Cbz, to amines.

PS-TBD is a polymer-supported base, which consists of a bicyclic guanidine moiety (1,5,7- triazabicyclo[4.4.0]dec-5-ene) anchored on polystyrene. PS-TBD is a stronger polymersupported base than the PS-DIEA and PS-NMM resins, which are bound tertiary amines that are used in organic synthesis and reagent scavenging. Published applications of PS-TBD include alkylation of phenolsi and amines;ii esterification of carboxylic acids using alkyl halides; alkylation of activated methylene compounds; de-halogenation of organic halides;iii high throughput synthesis of aryl triflates and aryl nonaflates,iv and the regioselective synthesis of lysophospholipids.

Product Notes

PS-DEAM is a polystyrene-bound diethanolamine, which is a resin-bound equivalent of the tridentate (N, O, O) N-alkyldiethanolamine ligand. PS-DEAM is an effective scavenger for a variety of oxophilic organometallic and inorganic complexes. The resin can be used to quench reactions and remove metallic reagents, catalysts or byproducts, allowing the purified product to be isolated by filtration. Boronic acids are scavenged by PS-DEAM. This has been exploited in the purification of Suzuki reactions through the removal of excess boronic acid and coupling byproducts.

Product Notes

MP-Borohydride is a macroporous, polystyrene-bound borohydride, that is a resin-bound equivalent of tetraalkylammonium borohydride. The bound borohydride is a versatile reducing agent1,2,3 used for the reduction of carbonyl compounds and imines, and the reductive amination of aldehydes and ketones. The resin, in conjunction with some transition metal salts, can also be used for a number of other important reductive applications, such as reduction of oximes, azides and alkyl halides. The reduced products are isolated by simple filtration away from the resin.

Product Notes