Application Notes

Application Notes

Java Runtime engine compatible with Pathfinder

Software

The ability to synthesize peptides in a range of different scales is a requirement in many research laboratories. The Biotage® Initiator+ Alstra™ fully automated microwave peptide synthesizer has a scale range of 5 μmol up to 2 mmol using three different reactor vial sizes (5 mL, 10 mL and 30 mL). The acyl carrier protein fragment, ACP (65–74), H-Val-Gln- Ala-Ala-Ile-Asp-Tyr-Ile-Asn-Gly-NH2 (VQAAIDYING-NH2) (1), is a well-known so-called difficult sequence and is commonly used to evaluate the performance of new synthesis reagents and instrumentation. Here we demonstrate a variable scale synthesis on the Initiator+ Alstra using the ACP (65–74) fragment as a model peptide.

Application Notes

Customer Case: Satori Pharmaceuticals. Extracting target compounds from large volumes of mixture confronts chemists with challenges which are right at the heart of large scale process chemistry. Ruichao Shen at Satori Pharmaceuticals knows exactly what it takes.

Customer Cases

This poster compares the extraction of the ethanol metabolites (alcohol biomarkers) from urine using strong anion exchange (EVOLUTE AX) and weak anion exchange (ISOLUTE NH2) solid phase extraction columns. A comparison with a simple dilution protocol is also presented. SOFT 2014

Alcohol metabolite Column English Ethyl Sulfate (EtS) Ethyl glucuronide (EtG) Forensic LC-MS/MS Posters Urine

Posters

In this customer case, Jonathan Tang at University of East Anglia in the UK explains how Biotage sample preparation products have been used successfully in his studies on the human skeleton.

Customer Cases

In the laboratory many chemicals are used in small and larger volumes. Table 1 shows several of the typical kinds of analyses done for environmental compliance. Semivolatile organic compound extraction and evaporation can be a large source of solvent emission to the atmosphere if the solvent is allowed to go up the hood and is not recaptured.

Product Notes

This application note describes the use of ISOLUTE® HM-N as a support material for pre-adsorption of a reaction mixture prior to flash chromatography. This approach is useful when loading reaction mixtures that are only soluble in polar solvents onto a Biotage® SNAP Ultra, SNAP KP-SIL or SNAP KP-NH column.

Application Notes

I receive many calls about the Speed-Vap III loosing vacuum and not working properly. Most likely your Speed-Vap III is not beyond repair.

Tech Tips

The focuses of this month’s discussion are the: “O” ring (30-0452-01) on the bottom of any 1000/3000 Disk Holder. Cap Adapters (150-0001, 150-0008-01) 40 mL VOA vial adapter (160-0001-02) 125 mL Sepratory Funnel (27-0834-01) Rinse Rods (50-0667) Gasket on the top of the water in valve (02-0560) “O” Rings used on the Rinse Rods – (30-0544, bag of 100).

Tech Tips

The focuses of this month’s discussion are the: Luer fitting (02-0592) on the bottom of any 4790 Disk Holder. Cap Adapters (150-0001, 150-0008-01) 40 mL VOA vial adapter (160-0001-02).

Tech Tips

Summary of Biotage offerings for Mass Directed Flash Chromatography

Brochures

In this study we synthesized two closely related amphipathic peptides and show the mass directed flash purification of non-acetylated ‘18A’ from a crude mixture containing a single residue deletion sequence ‘17A’ (-Glu residue) using the Biotage® Isolera Dalton™.

Application Notes

Here we show how flash chromatography with mass directed fractionation can be used for purifying carbohydrate derivatives such as β-D-glucosamine pentaacetate 1 as a model compound, and other UV transparent or poorly UV absorbing compounds using either normal or reversed-phase chromatography.

Application Notes

Developed by collaboration with the University of Eindhoven, European Regional Marketing and R+D, this application note describes the application of Isolera Dalton to the detection of multiply charged peptide ions prior to purification. This effectively increases the range of the Isolera Dalton mass detection, for this class of compounds and demonstrates that Isolera Dalton is a fully integrated purification instrument rather than a simple MS based analytical tool.

Application Notes English

In this poster we will show how using reversed-phase flash chromatography with the new Biotage® Isolera™ Dalton mass-directed flash purification system provides real-time simultaneous separation, detection, and identification of lipids. Presented at ACS Spring 2014.

Posters