Within EPA Method 549.2, the suggested rate to load a sample onto a solid phase cartridge is given as 3 to 6 mL/min. This Application Note outlines the process used to extract diquat and paraquat from water samples using a faster loading rate than given by the EPA in method 549.2

Application Notes Column Diquat Drinking water EPA 549.2 Analytes English Environmental HPLC Paraquat SmartPrep

This technical note describes the use of derivatization techniques to separate aliphatic secondary amines from aliphatic tertiary amines in a mixture using the strong cation exchange sorbent, ISOLUTE® SCX-2.

Application Notes Product Notes

Oftentimes, aqueous samples collected contain varying amounts of suspended solids or sediment strictly due to either the source of the water being sampled or improper sampling techniques. In any circumstance, samples with high amounts of particulates or sediment have proven challenging to extract using EPA Method 3535 Solid Phase Extraction (SPE).

4790 Application Notes Aqueous Disk English Environmental GC-MS SVOC Semi-Volatile Organics US EPA Method 3535A

Our carefully selected portfolio of industrial scale products have a proven track record of successful applications and use in scale-up projects. We can support the discovery, development and manufacturing of customer pharmaceutical and biotechnology products, from pre-clinical, phase I, II and III to small scale commercial operations.

Brochures English

The Biotage® Initiator is a safe, flexible microwave synthesizer for fast and scalable reactions, tailored for synthetic chemistry.

Product Notes

The Biotage® Initiator PeptideWorkstation is an accessory to perform manual microwave peptide synthesis and cleavage utilizing the Biotage Initiator microwave synthesizer.

Product Notes

The Initiator+ is a flexible system that utilizes all Biotage vials, from 0.2 to 20 mL, in any order or combination, at any time without system modifications, delivering greater flexibility and direct scale-up from milligrams to grams.

Product Notes

In peptide synthesis mode, this is the perfect tool for chemists synthesizing peptides and peptidomimetics, including difficult modifications and labeling of sequences, by using single or multi-step procedures. The intuitive Initiator 4.0 software controls the instrument functions and by using the pre-defined methods, synthesis can be started immediately.

Product Notes

Software

In mass detection, the presence of ions can suppress peaks from the analytes of interest. This study concludes that Isolera™ Dalton performs successful separations even with ions present in the sample.

Application Notes

Dioxins are of great environmental concern due to their teratogenic, mutagenic, and carcinogenic impact. These lipophilic compounds bioaccumulate in humans and wildlife and can have half lives of up to 132 years, making them persistent organic pollutants (POPs). Traditional SPE requires a filtration step to remove particulates when using SPE cartridges, because of clogging issues.

4790 Application Notes Dioxins English Environmental River Water Water

Peptide purification using standard reversed phase HPLC methods are hampered by low loading capacity, resulting in purifications that demand significant time investment. Recently, the use of reversed phase flash chromatography has increased in popularity for peptide purification due to the significant reduction of purification time, enabled by the increased loading levels of the larger stationary phase particles. Resolution, though, is somewhat diminished with the larger particle size, demanding creative techniques to retain a highly pure peptide product.

Posters

In medicinal chemistry, organic synthesis will generate by-products with similar chemistry to the product. Separating these impurities from the product often becomes a challenge that often only prep HPLC can achieve. In this poster, we discuss how using sample dry loading with a cation exchange scavenger media with flash chromatography improves product purity by removing many of the impurities.

Posters

Fragrance compounds, being invisible to UV, can be elusive and difficult to isolate. In this application, a four component mixture of fragrance compounds was purified using Accelerated Chromatographic Isolation™ (ACI) and identified by mass detection.

Application Notes

This Application Note reviews the use of ISOLUTE PPT+ plates for the isolation of drugs from biological fluids, including the method, recovery data and the impact of the filtrate’s cleanliness on analyte quantitation.

Application Notes

English Manuals & User Guides

Manuals & User Guides