Part No: AN059Issued year: 2012File size: 0.63mbFile type: pdf
Flash chromatography is the primary technology for separating, purifying, and isolating both synthetic organic compounds and natural products. Until recently, automated flash purification systems were limited to user-selected detection and collection wavelengths to fractionate separated compounds from sample mixtures. In this application note we demonstrate how the advances in Isolera Spektra are used in the purification of a spinach extract.
Part No: AN060Issued year: 2012File size: 1.02mbFile type: pdf
Isolera Spektra provides definitive fraction purity assessment
in both 2-D and 3-D prior to any post flash analysis. With this
information chemists quickly know if a fraction contains a pure
product suitable for further mass and structure confirmation
or whether the impure fractions must be re-purified. This
knowledge improves synthesis throughput and avoids any
embarrassment of submitting impure fractions for analytical
Part No: AN092.v1Issued year: 2014File size: 1.12mbFile type: pdf
The UV absorption spectrum of some solvents
overlaps with the product they dissolve, meaning
that fraction collection processes cannot distinguish
between solvent and product. Luckily, there is
technology that solves this problem.
Part No: AN095Issued year: 2014File size: 0.65mbFile type: pdf
Elution of polar molecules in flash chromatography sometimes requires solvent additives, making purification more cumbersome. The Isolera™ family of flash purification instruments and Biotage® SNAP KP-NH cartridges significantly reduce the extra work involved.
Part No: TN-0006.0806Issued year: 2006File size: 0.86mbFile type: pdf
Purify up to 400 grams of compound at 1 L/min with the Biotage Flash 150™ system, up to 2x faster than traditional glass columns. This robust stainless steel system safely operates at 100 psi enabling high flow rates and the use of higher viscosity solvents. A variety of cartridge media provides
chemists with selectivity choices for optimal purification conditions. Simple and reliable, this system contains everything needed to begin your separations.
Part No: TN407.1107Issued year: 2007File size: 0.07mbFile type: pdf
FlashVac-10 and -20 Sample Processing Manifolds are used
to process standard Luer tipped ISOLUTE work-up columns.
Constructed from glass or high density polyethylene, the
FlashVac manifolds are compatible with commonly used
reaction and work-up solvents.
Part No: Issued year: 2015File size: 1.88mbFile type: pdf
User Report: Isolera™ Dalton, Okayama University. The laboratory of Prof. Hiroyuki Miyachi at Okayama University installed Biotage’s Isolera Dalton, an automated mass-directed purification system for flash chromatography, allowing the fractionation and collection of optical isomers. “I felt like the world’s first mass-detection automated purification system had finally arrived.”
Part No: AN792Issued year: 2013File size: 2.3mbFile type: pdf
This application note describes fully automated liquid handling of the extraction of a range of drugs from urine, which are typically screened for forensic toxicology panels, using ISOLUTE® SLE+ 96-well supported liquid extraction plates.
drugs of abuse, TECAN, automated, ISOLUTE SLE+
Part No: AN115Issued year: 2018File size: 0.49mbFile type: pdf
This application note describes optimization for a fully automated synthesis and on-resin cyclization of oxytocin enabled by Branches™, a unique software feature of the Biotage® Initiator+ Alstra™ peptide synthesizer.
Part No: P150Issued year: 2016File size: 0.39mbFile type: pdf
Interest in peptide-based therapeutics has risen dramatically over the last decade. More specifically, significant energy has been dedicated to cyclic peptides, their synthesis, their biological activity, and their biological stability as potential alternatives to their linear counterparts. However, synthesizing a cyclic peptide creates significant challenges not encountered in linear peptide synthesis.
Herein we present a synthesis of oxytocin describing optimization for fully automated, on-resin disulfide bond formation utilizing Branches™, a unique feature enabling visualization and specific programing for cyclic and branched peptide synthesis. We will highlight the transferability of this optimized synthesis to larger scale syntheses and conclude with the fully automated synthesis of cyclized oxytocin incorporating an isotopically labeled Leu residue, enabling use as an internal standard in MS experiments.
Part No: TN113Issued year: 2006File size: 0.09mbFile type: pdf
ISOLUTE Confirm HCX columns are based on cation exchange and C8 mixed mode chemistries. Basic drugs are therefore retained by two primary retention mechanisms - ionic and non-polar. This allows a more rigorous interference elution regime to be used, leading to a very clean final extract, as many non-polar interferences which are retained by a non-polar interaction alone, can be eluted selectively, prior to elution of the drug.
Part No: P024Issued year: 2008File size: 1.9mbFile type: pdf
It is well known that traditional liquid-liquid extraction (LLE) provides very clean extracts prior to LC/MS analysis. Supported liquid extraction is analogous to traditional LLE, however, analyte partitioning takes place using an inert support material, rather than two immiscible liquids. This provides excellent extraction efficiencies while alleviating many of the tedious liquid handling issues associated with LLE.
Part No: TN116Issued year: 2006File size: 0.07mbFile type: pdf
The extraction of basic drugs from biological fluids using a purely non-polar retention mechanism (e.g. C4, C8 or C18) can lead to extracts that contain a large amount of non-polar co-extracted material that can interfere with subsequent analysis. Conversely, extraction mechanisms based on ion exchange interactions can be non-robust due to the variable ionic strength of the sample matrix.
Part No: TN129Issued year: 2005File size: 0.07mbFile type: pdf
The use of ISOLUTE HCX mixed-mode sorbents is widely accepted for providing high purity extracts of basic drugs from biological fluids. The ISOLUTE HCX-Q sorbent utilizes a combination of weak cation exchange and C8 non-polar retention mechanisms.
Part No: Issued year: 2014File size: 1.02mbFile type: pdf
Testing for drugs of abuse in oral fluids can strongly benefit the criminal justice field as a less invasive and cost-effective approach for drug detection when compared to blood or urine sampling. Oral fluid analysis has facilitated laboratory analysis for many drugs of abuse and is a constantly evolving analysis procedure which benefits from increasingly sensitive methods of detection. The Laser Diode Thermal Desorption (LDTD) source combined with Mass spectrometry is presented as a new screening tool for drug analysis in Oral Fluids. Analysis speed of LDTD provides accurate results in seconds in combination with exceptional specificity of MS instruments make a powerful platform for the screening of different drugs of abuse and new emerging drugs. Different extraction procedures are available; however those methods depend on specific drug conditions: basic or acid drugs / hydrophilic or hydrophobic. A new extraction approach, Supported Liquid Extraction (SLE+) is evaluated as generic extraction procedure.