Part No: AN747Issued year: 2011File size: 0.18mbFile type: pdf
Biotage® PRESSURE+ manifolds deliver positive pressure, parallel processing for 96 well plates and column formats. The systems utilize a consistent, uniform flow of positive pressure to move both low and high viscosity liquids through SPE plates and columns. ISOLUTE SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation and greatly matrix effects associated with biofluid analysis.
SLE+, SLE, Supported Liquid Extraction, positive pressure manifold, Pressure+96, Pressure+48
Part No: UI333Issued year: 2015File size: 0.89mbFile type: pdf
If high back pressure is detected in the makeup pump lines, and/or the High Makeup Flow Pressure dialog appears, the microfilter may be partially clogged and needs replacing. This is a step by step guide.
Part No: UI403Issued year: 2020File size: 0.53mbFile type: pdf
Biotage® Sfär columns can be used with CombiFlash Rf and
CombiFlash NextGen Systems. This document outlines how to
set up your CombiFlash instrument for use with Biotage® Sfär
Silica HC and Biotage® Sfär C18.
Part No: UI319.v3Issued year: 2017File size: 0.7mbFile type: pdf
Biotage®SNAP Ultra cartridges are compatible with CombiFlash® Rf Systems. As a convenience, Biotage SNAP Ultra cartridges with Luer slip connectors are available. Contact your Biotage representative for ordering information.
Part No: TechTipIssued year: 2011File size: 0.07mbFile type: pdf
Are you keeping up with your annual six month Hydrophilic Solution treatments for your SPE-DEX 4790 or 1000/3000 SPE-DEX instruments?
1. The water sample will not flow down into the disk holder.
2. My water inlet valve may not be opening
3. My water inlet valve is opening, but my sample is not dispensing into the disk holder.
4. My entire collection vessel is filled with the water sample.
Part No: AN078Issued year: 2013File size: 1.05mbFile type: pdf
In this application note we demonstrate the power of mass detection in Isolera™ Dalton by investigating the incomplete separation of two dyes, Butter yellow (Mass 225.29 g/mol) and Sudan red (Mass 278.31 g/mol), by normal phase flash purification.
Part No: P053Issued year: 2013File size: 1.22mbFile type: pdf
Immunosuppressant drugs are extremely important for therapeutic drug monitoring (TDM) regimes, requiring robust and reliable methods for their analysis. This poster aims to demonstrate strategies for the extraction of various immunosuppressant drugs from whole blood providing clean samples prior to UPLC-MS/MS analysis. Spiked whole blood was extracted using a variety of protocols to investigate optimal combination of drug recovery and extract cleanliness. UPLC-MS/MS was conducted using a Waters ACQUITY UPLC coupled to a Quattro Premier XE triple quadrupole mass spectrometer. Acceptable extraction recoveries were obtained showing excellent extract cleanliness, demonstrating a reliable method for mass spectrometric approaches for these analytes.
Immunosuppressants, SLE, Supported Liquid Extraction, ISOLUTE, MSACL, San Diego, 2013
Part No: P140Issued year: 2016File size: 0.31mbFile type: pdf
Reversed-phase chromatography is typically used when you need to separate several milligrams of relatively polar compounds that either are not soluble in normal-phase solvents or are not compatible with bare silica
because they react, stick, or both. If you are currently using reversed-phase at preparative scale, such as flash chromatography, you know the mobile phase limitations – water with either methanol, acetonitrile, or
THF. As with normal-phase flash chromatography, when it comes time to purify you want your crude sample fully solubilized in the weakest possible solvent at the highest possible concentration.
Part No: AN044-HORIssued year: 2009File size: 0.65mbFile type: pdf
The Biotage® DryVap Concentrator System was intentionally designed to provide enhanced recoveries of semivolatile organic compounds (SVOC) such as those found in EPA Method 8270. The unique design of the DryVap system, which uses a vacuum, sparge gas, and internal immersion heater, allows it to achieve excellent recoveries for a wide range of compounds.
This purpose of this study is to determine the washing
technique for a DryVap® concentrator tube which
optimizes the recovery of both low and high boiling point
Part No: AN075.v2Issued year: 2013File size: 0.49mbFile type: pdf
In this application note we present a new type of polymeric reverse phase adsorbent that is able to provide improved separations of a range of small chemical compounds and also peptides evaluated side by side to a comparable benchmark DVB-Styrene macroporous resin.
MIP, molecularly imprint resins
Part No: AN124-HORIssued year: 2017File size: 1.83mbFile type: pdf
Semivolatile organic compounds (SVOC) have a variety of chemical properties that have been found to cause harmful effects to both humans and the environment. Accurate measurements are challenging to obtain because SVOCs readily adsorb onto surfaces, and are found in common household items such as cleaning agents, personal care products, electrical components, pesticides, water and food.
Part No: AN095-HORIssued year: 2014File size: 2.85mbFile type: pdf
The global demand for fish as a natural source of fresh animal protein, essential fats, minerals, and vitamins continues to rise with the human population. It is estimated that natural fish resources will not be sustainable, creating the need to increase the available supply of fish through aquaculture.
Part No: AN106-HORIssued year: 2016File size: 1.4mbFile type: pdf
Worldwide, there is great concern for the presence of semi-volatile organic compounds in surface water, groundwater, and drinking water, such as Aldrin, extremely toxic to fish and a persistent organic pollutant. China has increased concern about drinking water as sources of surface and groundwater have become polluted with microbiological, metal and organic contamination
Part No: AN063Issued year: 2012File size: 0.48mbFile type: pdf
Pyrazines are a class of organic molecules often used to provide flavor to foods. They are typically synthesized but some are found in fruits and vegetables, e.g. grapes, bell peppers, peas, asparagus, beetroot, tobacco, and roasted foods. Pyrazine’s heterocyclic chemistry can yield some challenges to their purification due to the various separation kinetics between the compound and silica. Biotage SNAP Ultra.
Part No: AN123-HOR.V.1Issued year: 2017File size: 2.46mbFile type: pdf
The US EPA monitors a variety of chemicals in water that may cause harm to humans or wildlife in order to minimize exposure. Method 625 was developed by the Office of Science and Technology in the Clean Water program to monitor a large suite of semivolatile chemicals in wastewater for compliance with the National Pollution Discharge Elimination System (NPDES). NPDES is a system of permitting that regulates the characteristics of water that is released into a waterway, defined by industrial category. The permitting levels are set depending on the waterway’s use.