Whether needing to reduce THC to acceptable levels for commercial CBD sales or purifying individual cannabinoids such as CBG or CBN, flash chromatography can provide an effective way to accomplish these goals.

Manuals & User Guides White Papers

Peptide purification via flash chromatography has recently been demonstrated as a viable alternative to the more standard HPLC methods currently utilized. Flash chromatography offers peptide chemists the advantage of significantly greater loading capacity reducing the overall purification time but with a compromise of decreased peak resolution. Herein we present several strategies that, when implemented, allow for very high purity peptide samples purified by flash chromatography.

White Papers

This white paper has discussed design criteria for an effective high-performance microwave for chemical synthesis. Criteria have been outlined for the safe and effective operation or any system that superheats chemical reactions, and the reasons for these requirements have been discussed. Using these considerations Biotage have developed their Initiator+ system.

White Papers

Analysis of drug panels in urine samples can be challenging, and the trend towards larger panels including multiple drug classes compounds the issues faced during method development. This white paper examines a number of aspects of sample preparation, and their impact on the success of subsequent LC-MS/MS analysis of broad urine panels. Section 1 examines the applicability of various sample preparation techniques: supported liquid extraction, reverse phase SPE and mixed-mode SPE, to the various classes of drugs extracted. In addition, hydrolysis approaches: enzyme type and protocol used (time, temperature), are compared. Mixed-mode reverse phase/cation exchange SPE is widely used for extraction of basic drug classes from urine, but the inclusion of drugs and metabolites that exhibit ‘non-typical’ functionality within urine panels can be problematic. Section 2 examines the impact of various parameters (interference wash strength, elution solvent composition) on analyte retention, elution and extract cleanliness with particular focus on zwitterionic (gabapentin, pregabalin) and non-ionic (carisoprodol, meprobamate) drugs.

11-nor- Δ9-carboxy tetrahydrocannabinol (THC-COOH) 6-acetylmorphine (MAM) 7-Aminoclonazepam 96-well plate Alprazolam Amitriptyline Amphetamine Amphetamines Anaesthetic Anticonvulsant Application Notes Barbiturates Benzodiazepines Benzoylecgonine Buprenorphine Butalbital Cannabinoid Carbamate hypnotic Carbamate muscle relaxant Carbamates Carisoprodol Chlordiazepoxide Clinical Clonazepam Cocaine Cocaines Codeine Diazepam Dihydrocodeine (DHC) EDDP English Fentanyl Forensic Gabapentin Hallucinogen Hydrocodone Hydrolysed urine Hydromorphone Ketamine LC-MS/MS Lorazepam MDMA Meperidine Meprobamate Methadone Methamphetamine Morphine N-des-tapentadol Naloxone Norbuprenorphine Nordiazepam Norfentanyl Norhydrocodone Norketamine Normeperidine Noroxymorphone Norpropoxyphene Nortryptiline O-desmethyltramadol Opiates Opioid Opioid agonist Oxazepam Oxycodone Oxymorphone Pentobarbital Phencyclidine Phencyclidine (PCP) Phenobarbital Pregabalin Product Notes Ritalinic acid Secobarbital Stimulants Sympathomimetic amine THC metabolites THC-COOH Tapentadol Temazepam Tramadol Tricyclic Antidepressant White Papers Zolpidem Zolpidem-phenyl-4-COOH z-drugs α-OH alprazolam

Die Flash-Chromatographie ist die bevorzugte Reinigungsmethode für organische Stoffe, Arzneimittel und Naturstoffe. In jüngster Zeit hat sie auch die Peptidchemie erobert, verfügt sie doch über die Fähigkeit, eine Vielzahl unterschiedlicher Verbindungen effizienter zu trennen, als dies mit anderen Vorreinigungsverfahren wie z. B. dem Ausfällen (Protein-Crash) oder der Flüssig-Flüssig-Extraktion möglich ist. Zur Herstellung reiner Verbindungen können Chemiker je nach dem gewünschten Reinheitsgrad auf eine Vielzahl unterschiedlicher Variablen zurückgreifen. In diesem Whitepaper möchten wir die Faktoren erläutern, die für eine erfolgreiche Aufreinigung mithilfe der Flash-Chromatographie kontrolliert werden müssen.

White Papers

For as long as column chromatography has been practiced by organic and medicinal chemists there has been a concern that too much methanol in the solvent system will dissolve the column’s silica. This concern has come about because chemists often find a solid white precipitate in collected fractions after purification. This phenomenon is usually noticed when dichloromethane and methanol are used as the mobile phase solvents. In this poster we will show the results of a study that evaluated the degree of dissolution for both granular and spherical silica in methanol and dichloromethane.

English Pharma Posters White Papers

In diesem Whitepaper sollen verschiedene Aspekte der Probenvorbereitung beleuchtet werden. Dazu gehört auch der Einfluss der Probenvorbereitung auf den Erfolg der nachfolgenden LC-MS/MS-Analyse von breit angelegten Urin-Panels.

White Papers

It is imperative that pharmaceutical processes have the flexibility and robustness to quickly output targets of interest – from small molecules and APIs for drug products to complex structures or chemical sensors that may be used in kits or other diagnostic devices. In chemical manufacturing, for example in the pharmaceutical industry, target molecules are synthesized on a large scale in dedicated manufacturing plants. Such processes represent the culmination of a great deal of research and development, where much time and consideration has been spent scaling laboratory based synthetic routes into commercially and practically viable manufacturing procedures. This paper explains the principles of scale up purification.

White Papers

The Biotage approach to large-scale purification in the manufacturing environment is based around a practical, scalable approach to flash chromatography. Methods are developed in the laboratory using dedicated equipment, and the methods are scaled up using a simple scale up procedure based on utilizing the same media and simply scaling up the column size, flow rate and loading appropriately, to generate identical results.

White Papers

Oral fluid is of increasing importance as a biological matrix in drugs of abuse and related analyte testing because of the ease of sample collection, and difficulty in adulteration of samples, particularly when compared with urine.

Application Notes Drugs of Abuse English Forensic LC-MS/MS Oral fluid Saliva White Papers

Producing peptides for research is challenging. Biotage has developed a holistic approach to the entire peptide workflow via an automated solution, designed for dedicated peptide researchers and those new to the field.

Manuals & User Guides Product Notes White Papers

Modelle haben gezeigt, dass sich die zur Herstellung eines Zielmoleküls benötigte Zeit mit dem automatisierten Workflow von Biotage um bis zu achtzig Prozent verkürzen lässt, sodass auch die Projektlaufzeiten entsprechend verkürzt werden können.

German Product Notes White Papers

Traditional approaches to compound purification involving chromatography utilize large volumes of relatively toxic and expensive solvents, and significantly contribute to the environmental footprint of organizations involved in molecular research. Current directives for greener chemistry have put pressure on organizations to reduce the environmental impact of their work.

Environmental Product Notes White Papers

Bei herkömmlichen Aufreinigungsverfahren mittels Chromatographie kommen große Mengen verhältnismäßig giftiger und teurer Lösungsmittel zum Einsatz, die sich negativ auf den ökologischen Fußabdruck von Unternehmen in der Molekularforschung auswirken. Neue Richtlinien für einen verantwortungsvolleren Umgang mit Chemikalien zwingen Unternehmen, die Umweltauswirkungen ihrer Arbeit zu reduzieren. Durch Einblicke in die Arbeitsabläufe dieser Unternehmen und neue Entwicklungen im Bereich Flash-Chromatographiesysteme und -säulen lassen sich Verbrauch und Toxizität der für die Aufreinigung eingesetzten Lösungsmittel und somit auch die Umweltbelastungen dieser Verfahren reduzieren.

Environmental German Product Notes White Papers

Using the Biotage automated workflow, models have shown that the time taken to produce a target molecule can be reduced by up to eighty percent, allowing project delivery timescales to be reduced accordingly.

Product Notes White Papers