Part No: AN003-HORIssued year: 2009File size: 0.86mbFile type: pdf
This study shows that the SPE-DEX® 4790 Automated Extractor system can process water samples containing suspended particulate matter, up to 1000 mg, for the analysis of PAH’s. As PAH’s are found in both the dissolved and particulate phase of the water sample, it is important to be able to handle “whole” water samples such that the particulate matter in the water sample is retained and extracted with the SPE disk. This filtration method ensures that the PAH’s found in the dissolved phase, and those adsorbed onto the particulate matter are properly
Part No: AN863Issued year: 2016File size: 1.62mbFile type: pdf
This application note describes the use of ISOLUTE® ENV+ on-line cartridges in a fully automated on-line SPE-LC-MS/MS method for extraction and quantification of 16 acid herbicides in drinking and surface water.
Part No: SSI-LCMS-096Issued year: 2018File size: 0.87mbFile type: pdf
Analysis of the perfluorinated alkyl acids listed in EPA Method 537 along with fluorotelomeric alcohols and perfluorinated sulfonates were extracted using SPE (using ISOLUTE 101 500 mg/ 6 mL SDVB SPE columns) and analyzed on the LCMS 8045. Minimum Detection Limits(MDL) ranged from 0.69 to 3.25 ppt. Extraction recoveries were greater than 80% for all compounds, with surrogate recoveries within 10% of the true value.
Part No: AN080-HORIssued year: 2015File size: 1.18mbFile type: pdf
This application note demonstrates the use of automated solid phase extraction for a variety of pharmaceutical compounds in drinking water,
wastewater and surface water. The criteria specified in US EPA Method 1694 was met, even at very low concentrations. For lower concentrations a larger sample of water may be processed.
Part No: AN862Issued year: 2016File size: 1.48mbFile type: pdf
This application note describes the use of EVOLUTE® EXPRESS ABN on-line cartridges in a fully automated on-line SPE-LC-MS/MS method for extraction and quantification of 13 commonly prescribed pharmaceuticals (with a range of characteristics) in water.
Part No: AN703Issued year: 2011File size: 0.04mbFile type: pdf
ISOLUTE EPH SPE columns and associated methodologies have been optimised to efficiently fractionate EPHs into aliphatic and polycyclic aromatic (PAH) fractions. The principle is similar to the approach taken by the Massachusetts Department of Environmental Protection (MADEP) and TPH criteria working group (TPHCWG) methods. However, compared to these methods, the ISOLUTE EPH fractionation column has been significantly reduced in size and the sorbent has been optimised in order to reduce solvent volumes and provide an automation compatible fractionation column. Automation of the fractionation process has been achieved without carryover of PAHs into the aliphatic fraction.
Environmental contamination, ISOLUTE, PAH, Polyaromatic hydrocarbons, soil,
Part No: AN045-HORIssued year: 2009File size: 0.65mbFile type: pdf
In this application note, a suite of neutral and list 1 pesticides in methylene chloride is evaporated using the Biotage® DryVap.
Pesticides containing many different functional groups, including sulfur and phosphorus groups, halides, nitramines, atrazines, alcohol, and carbonyl groups were efficiently concentrated for GCMS analysis.
This data indicates that the DryVap System is inert and effectively manages solvent evaporation without generating localized hot spots in the system.
Part No: AN877Issued year: 2017File size: 2.61mbFile type: pdf
This application note describes the operating conditions for the automated fractionation of Extractable Petroleum hydrocarbons (EPH) into aliphatic and aromatic (PAH) fractions using the ISOLUTE EPH SPE column in conjunction with the Extrahera automation system.
Part No: AN110-HORIssued year: 2016File size: 2.15mbFile type: pdf
This work demonstrates the performance of automated solid phase extraction (SPE) for the extraction of PAHs from surface water sampled from a nearby pond containing some particulate. An automated system (Biotage® Horizon 4790) with Atlantic® DVB disks, following the requirements of method EN 16691, was used.
Part No: AN109-HORIssued year: 2016File size: 1.5mbFile type: pdf
This application note describes the automated solid phase extraction (SPE) and GC/MS analysis of whole water samples according to EN16691 for PAHs.
Method EN 16691 is a recently developed method that uses solid phase extraction to isolate organic compounds from 1 L of water using a divinylbenzene (DVB) solid phase extraction disk.
Part No: AN122-HORIssued year: 2017File size: 1.87mbFile type: pdf
This application note demonstrates the performance of automated disk-based solid phase extraction (SPE), using Biotage® Horizon 5000 automated extraction system and Atlantic® DVB SPE disks, for concentration of organochlorine pesticides from whole water, prior to GC/MS analysis, following the requirements of EN16693:2015.
Part No: AN908.V.1Issued year: 2014File size: 1.33mbFile type: pdf
The removal of interferences and sample concentration are
essential steps in trace level detection of pesticides, sheep
dips, herbicides and PCBs in river water, wastewaters and
trade effluents. The following methods were developed by
the Environment Agency National Laboratory Service facility
at Nottingham using the Biotage RapidTrace for automated
solid phase extraction and the TurboVap II for evaporation.
The procedures are UKAS accredited and can reach detection
limits of 0.001 ug/Litre for some compounds.
Part No: AN904.V1Issued year: 2014File size: 1.33mbFile type: pdf
EPA methods 8260 & 8270 cover the analysis of polychlorinated
dibenzo-p-dioxins and polychlorinated dibenzofurans
in ground & surface waters. This also now includes
1,4-dioxane and PCBs. This application note will focus on
the automated SPE of the sample rather than the detection
which usually involves GCMS or LCMS. NB The columns
and reagents used for soils and sediment analysis differ to
ground water extraction.
Part No: AN002-HORIssued year: 2015File size: 0.94mbFile type: pdf
This study shows that the Biotage® 4790 Automated Extractor system, along with the Atlantic™ DVB disk, the DryDisk® separation membrane, and the DryVap® Concentrator System can be successfully used to analyze for Nonyl Phenol and Bisphenyl A in water samples. This sample preparation process is fully automated, requiring minimal human intervention, and allowing for reductions in sample processing costs.
Part No: AN022-HORIssued year: 2009File size: 0.91mbFile type: pdf
EPA Method 1657 describes the procedure to determine low ppb levels of organophosphorous pesticides in wastewater.
The disks compared in this study are: “Certified for Automation” Atlantic™ C18 (47 mm) and 3M Empore™ High Performance Extraction Disk C18 (47 mm).
Part No: AN018-HORIssued year: 2010File size: 0.9mbFile type: pdf
EPA Method 608 describes the procedure to determine low parts per billion levels of organochlorine pesticides, polychlorinated biphenyls, toxaphene and chlordane in wastewater. The laboratory that collected the data presented in this application note uses EPA method 608 ATP3M0222 as the approved SPE technique for waste water extraction.
The disks compared in this study are : “Certified for Automation” Atlantic™ C18 (47 mm) and 3M Empore™ High Performance Extraction Disk C18 (47 mm). Comparisons were made using side-by-side extraction and identical calibration parameters.
Part No: AN008-HORIssued year: 2009File size: 0.89mbFile type: pdf
EPA Method 525.2 describes the procedure to determine
low ppb levels of semi-volatile organic material in drinking
water using solid phase extraction (SPE) or liquid-solid
extraction (LSE) techniques. The extraction solvents are
methylene chloride and ethyl acetate. Extracts are analyzed
by GC/MS using a splitless injection technique.