This application note describes the extraction of a range of SPICE drugs and metabolites in urine which are typically screened in forensic toxicology panels using ISOLUTE® SLE+ in a 96-well plate format. Both manual (Biotage Pressure+ 96) and automated (TECAN Freedom EVO® 100) processing conditions are described.

96-well plate Application Notes English Forensic JWH 018-N-(4-hydroxypentyl) JWH-018 JWH-018 JWH-018-(5-hydroxypentyl) JWH-018-N-5-(pentanoic acid) JWH-073 JWH-073-N-(3-hydroxybutyl) JWH-200 JWH-250 JWH-250-N-(5-hydroxypentyl) LC-MS/MS Synthetic Cannabinoids (SPICE) TECAN UR-144 UR-144-(5-hydroxypentyl) UR-144-(pentanoic acid) UR144-(5-chloropentyl) Urine XLR-11

This application note describes the extraction and quantitation of Cannabimimetic Naphthoylindoles (Synthetic Cannabinoids) and their metabolites (JWH Series) from various matrices using Supported Liquid Extraction (SLE). Synthetic Cannabinoids or SPICE as they are commonly known have become an increasing problem as one of the newest forms of illicit drugs being consumed today. These compounds bind to the cannabinoid receptors in mammals triggering similar euphoric symptoms as Tetrahydrocannabinoids (THC). Currently robust and fast analytical methods of analysis are required to aid in the screening and detection of this growing class of compounds. The recoveries obtained for the synthetic cannabinoids parent and metabolites ranged from 70-98 %. Supported Liquid Extraction, SLE, SLE+, SPICE, Synthetic Cannabinoids, JWH, metabolites,

Application Notes Column Forensic JWH 018-N-(4-hydroxypentyl) JWH-018 JWH-018-N-5-(pentanoic acid) JWH-073 JWH-073-N-(3-hydroxybutyl) JWH-200 JWH-250 JWH-250-N-(5-hydroxypentyl) JWH019 LC-MS/MS Plasma Synthetic Cannabinoids (SPICE) Urine Whole blood

This application note describes the extraction of a range of SPICE drugs and metabolites from neat oral fluid and oral fluid from a commercial collection kit using ISOLUTE® SLE+ in both 96-well plate and column formats. SPICE, oral fluid, Intercept Orasure, LC-MSMS, SLE

96-well plate Application Notes Column English Forensic JWH 018-N-(4-hydroxypentyl) JWH-018 JWH-018-N-5-(pentanoic acid) JWH-073 JWH-073-N-(3-hydroxybutyl) JWH-200 JWH-250 JWH-250-N-(5-hydroxypentyl) LC-MS/MS Oral fluid Synthetic Cannabinoids (SPICE) UR-144 UR-144-(5-hydroxypentyl) UR-144-(pentanoic acid) UR144-(5-chloropentyl) XLR-11

This application note describes the extraction of a range of synthetic cannabinoids and metabolites from oral fluid collected using the Quantisal™ Oral Fluid Collection Device prior to GC-MS analysis. An effective and efficient ISOLUTE® SLE+ protocol has been developed that is optimized for loading volumes of either 400 μL or 1 mL of matrix. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 80% with RSDs <10% for all analytes.

AM-2201 Application Notes Column English Forensic GC-MS JWH 018-N-(4-hydroxypentyl) JWH-018 JWH-018-(5-hydroxypentyl) JWH-073 JWH-073-N-(3-hydroxybutyl) JWH-200 JWH-250-N-(5-hydroxypentyl) Oral fluid Saliva Synthetic Cannabinoids (SPICE) UR-144

This application note describes the extraction of a range of synthetic cannabinoids and metabolites from whole blood prior to GC-MS analysis. An effective and efficient ISOLUTE® SLE+ protocol has been developed that is optimized for extraction of 800 μL of pre-treated matrix. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 85% with RSDs lower than 10% for all analytes.

AM-2201 Application Notes Column English Forensic JWH 018-N-(4-hydroxypentyl) JWH-018 JWH-018-(5-hydroxypentyl) JWH-073 JWH-073-N-(3-hydroxybutyl) JWH-200 JWH-250-N-(5-hydroxypentyl) LC-MS/MS Synthetic Cannabinoids (SPICE) UR-144 Whole blood

Drug of abuse testing is typically conducted using urine specimens from patients being screened for illicit drug use. While collection of urine is considered a relatively non-invasive drug testing action, it is not convenient for law enforcement to do in the field. The ability to collect an oral fluid sample in the field can be considered non-evasive and simple to implement. Currently there are oral fluid collection kits (e.g. Immunalysis Quantisal™, Orasure Technologies Intercept®) that facilitate the collection in easy to follow protocols. Oral fluid samples collected using standard collection kits can be qualitatively and quantitatively analyzed by LC-MS/MS, post extraction of the target analytes from the matrix. Drug analytes can be successfully extracted from oral fluid using Supported Liquid Extraction (ISOLUTE SLE+), which offers an efficient alternative to traditional liquid-liquid extraction (LLE). In this poster, we demonstrate a new rapid and reliable sample preparation method to extract a broad suite of drugs (Figure 1) from neat oral fluid and buffered oral fluid matrices. MSACL 2014

6-acetyl codeine 6-acetylmorphine (MAM) 96-well plate Alprazolam Benzodiazepines Buprenorphine Clonazepam Codeine Column Diazepam Drugs of Abuse EDDP English Fentanyl Flunitrazepam Forensic Hydrocodone JWH 018-N-(4-hydroxypentyl) JWH-018 JWH-018 JWH-018-N-5-(pentanoic acid) JWH-073 JWH-073 JWH-073-N-(3-hydroxybutyl) JWH-200 JWH-250 JWH-250-N-(5-hydroxypentyl) LC-MS/MS Morphine Naltrexone Nitrazepam Normeperidine Oral fluid Oxazepam Oxycodone Oxymorphone Posters Synthetic Cannabinoids (SPICE) Temazepam UR-144 UR-144-(5-hydroxypentyl) UR-144-(pentanoic acid) XLR-11

This application note describes effective and efficient protocols optimized for sample loading volumes of either 400 μL or 1 mL using ISOLUTE SLE+ supported liquid extraction columns. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 83% with RSDs of <10% for all analytes.

AM-2201 Application Notes Column English Forensic GC-MS Hydrolysed urine JWH 018-N-(4-hydroxypentyl) JWH-018 JWH-018 JWH-018-(5-hydroxypentyl) JWH-073 JWH-073 JWH-073-N-(3-hydroxybutyl) JWH-200 JWH-250-N-(5-hydroxypentyl) Synthetic Cannabinoids (SPICE) UR-144

This application note describes the extraction of a range of synthetic cannabinoids (SPICE) and metabolites, using ISOLUTE SLE+ supported liquid extraction columns, from oral fluid matrix collected using the NeoSal™ devices, prior to GC/MS analysis.

AM-2201 Application Notes Clinical Column English Forensic GC-MS JWH 018-N-(4-hydroxypentyl) JWH-018 JWH-018 JWH-018-(5-hydroxypentyl) JWH-073 JWH-073 JWH-073-N-(3-hydroxybutyl) JWH-200 JWH-250-N-(5-hydroxypentyl) Oral fluid RCS-4 Saliva Spice Synthetic Cannabinoids (SPICE) UR-144