Part No: AN818.v1Issued year: 2014File size: 1.54mbFile type: pdf
This application note describes the extraction of ethyl glucuronide and ethyl sulfate from human urine using ISOLUTE® NH2 solid phase extraction columns. The method has been applied real patient samples that had been previously analyzed with a validated referee method. The results of the orthogonal measurements agreed, to provide similar diagnostic values.
Part No: PPS451Issued year: 2017File size: 2.99mbFile type: pdf
Whether you need targeted methods for analytes such as Vitamin D metabolites in serum, or methods suitable for extraction of a wide panel of drugs and metabolites from urine, sample preparation before analysis is essential.
This compendium highlights a selection of clinical sample preparation applications from Biotage.
Part No: P045Issued year: 2012File size: 0.32mbFile type: pdf
This poster will demonstrate SPE sample preparation strategies for the simultaneous extraction of both ethyl glucuronide and ethyl sulphate from urine, allowing cleaner extraction protocols compared to traditional dilute and shoot approaches.
EtG, EtS, Urine, Poster, SOFT, Forensic, UPLC, LC-MS/MS, EVOLUTE, AX, WAX
Part No: AN748Issued year: 2011File size: 0.23mbFile type: pdf
Ethyl glucuronide (EtG, Figure 1.) is an ethanol metabolite formed by the glucuronidation of the parent molecule. The presence of EtG in urine and plasma is a useful and sensitive biomarker of alcohol intake in forensic toxicology. EtG can be detected at very low levels and is useful in distinguishing between ingested ethanol and that which is adsorbed through the skin from ethanol. This application note has been optimized on the RapidTrace+; an updated version of the current RapidTrace software which allows the system to process smaller bed heights than were previously possible. The application note shows analyte recoveries all above 97% with RSDs >10%.
EVOLUTE, Resin, EtG, Alcohol, Alcohol Biomarker, metabolite, anion exchange, Strata X, Oasis, Waters,
Part No: AN876.V.1Issued year: 2017File size: 2.77mbFile type: pdf
Phosphatidylethanol is an alcohol biomarker with a high degree of specificity; blood concentration of PEth correlates to the amount of alcohol consumed. This application note describes the extraction of 3 common species of PEth from whole blood using ISOLUTE® SLE+ supported liquid extraction prior to HPLC-MS/MS analysis.
The method is easily automated using the Biotage® Extrahera, and details of the automated procedure are included in the application note.
Part No: P115Issued year: 2014File size: 2.69mbFile type: pdf
This poster compares the extraction of the ethanol metabolites (alcohol biomarkers) from urine using strong anion exchange (EVOLUTE AX) and weak anion exchange (ISOLUTE NH2) solid phase extraction columns. A comparison with a simple dilution protocol is also presented.
Part No: AN755Issued year: 2012File size: 1.03mbFile type: pdf
Ethyl glucuronide (EtG) and ethyl sulphate (EtS) are specific metabolites formed within the body after the ingestion of alcohol. In forensic toxicology, when tested together they provide more specific evidence of alcohol intake. This application note demonstrates the simultaneous extraction of both EtG and EtS in the same assay and represents further optimization of AN718 which showed single EtG extraction without EtS. Recoveries for EtG range from 90-105% and 80-103% for EtS across two formats of EVOLUTE AX columns with RSDs <10%. The LOQ is 10 ng/mL for EtG and 2 ng/mL for EtS across both EVOLUTE AX formats.
EVOLUTE AX, EVOLUTE, EtS, EtG, Ethyl Glucuronide, Ethyl Sulfate, SPE, Anion Excange, Urine, OASIS, Waters, Polymeric, Polmers,