Part No: AN758Issued year: 2012File size: 1.22mbFile type: pdf
Immunosuppressants inhibit or prevent the activity of the immune system used to prevent rejection of transplanted organs and treat autoimmune diseases. Due to the risks of immunodeficiency, those under treatment must undergo constant therapeutic drug monitoring (TDM) requiring reliable and robust analytical techniques for quantification of these drugs. Current methodologies use immunoassay techniques to measure immunosuppressant levels in patients which are expensive, time consuming and susceptible to issues with cross reactivity. The sample prep method in this application note offers an alternative approach to immunosuppressant analysis with LC-MS/MS giving more reliable and robust recoveries with no cross-reactivity and cost saving opportunities. Analyte recoveries range from 60-97% with RSDs all below 10%.
Immunosuppressant, Tacrolimus, Sacrolimus, Everolimus, Cyclosporin A, Clinical, SLE+, SLE, Supported Liquid Extraction, immune, Clinical, Whole Blood, immunoassay,
Part No: AN810Issued year: 2014File size: 1.13mbFile type: pdf
This application note describes the extraction of the immunosuppressant drug mycophenolic acid and its metabolite mycophenolic acid glucuronide from fortified serum using ISOLUTE SLE+ in a 96-well plate format.
Part No: P076Issued year: 2014File size: 0.41mbFile type: pdf
Immunosuppressant drugs are instrumental in preventing organ and tissue rejection in patients undergoing transplant surgery. Mycophenolic Acid is a common immunosuppressant drug used in patient transplant therapy. The ability to monitor the trough levels in patients to evaluate dosing is important for the administering of the drug. The free drug and its glucuronidated metabolite can be found in patient serum. The ability to extract the free drug and metabolite in patient serum is supported by using a fast and efficient sample preparation extraction method called Supported Liquid Extraction (ISOLUTE SLE+). The free drug and metabolite can be recovered from serum using ISOLUTE SLE+ with high enough efficiency to allow for quantitation at target trough levels.
Part No: P084Issued year: 2014File size: 0.72mbFile type: pdf
This poster describes the extraction of the commonly used immunosuppressant drug mycophenolic acid and its glucuronide metabolite from serum using ISOLUTE SLE+ supported liquid extraction plates.
Part No: P053Issued year: 2013File size: 1.22mbFile type: pdf
Immunosuppressant drugs are extremely important for therapeutic drug monitoring (TDM) regimes, requiring robust and reliable methods for their analysis. This poster aims to demonstrate strategies for the extraction of various immunosuppressant drugs from whole blood providing clean samples prior to UPLC-MS/MS analysis. Spiked whole blood was extracted using a variety of protocols to investigate optimal combination of drug recovery and extract cleanliness. UPLC-MS/MS was conducted using a Waters ACQUITY UPLC coupled to a Quattro Premier XE triple quadrupole mass spectrometer. Acceptable extraction recoveries were obtained showing excellent extract cleanliness, demonstrating a reliable method for mass spectrometric approaches for these analytes.
Immunosuppressants, SLE, Supported Liquid Extraction, ISOLUTE, MSACL, San Diego, 2013
Part No: AN728Issued year: 2011File size: 0.15mbFile type: pdf
Cyclosporine (Figure 1) is an immunosuppressant drug most significantly used in post-operative organ transplants to reduce the chance of rejection of donor organs by decreasing the activity of the patient’s immune system. Subsequent to an organ transplant, patients are tested at regular intervals for the presence of very low levels of the compound. Cyclosporine is a cyclic non-ribosomal peptide of 11 amino acids and contains a single D-amino acid.
Cyclosporine, Cyclosporine, Immunosuppressant,
Part No: AN926Issued year: 2019File size: 0.93mbFile type: pdf
In this application note, ISOLUTE® SLE+ was used as a sample pretreatment column for mycophenolic acid determination in plasma using supported liquid extraction (SLE). UV based methods are commonly used to measure mycophenolic acid. In this application note, we have developed a method that can be used for both UV measurement and LC/ MS/MS measurement. LC/MS/MS assays are highly selective and less susceptible to the effects of other drugs used in combination during treatment.