Part No: AN843Issued year: 2015File size: 0.86mbFile type: pdf
Ethylestrenol and stanozolol are anabolic steroids which can be used to increase muscle mass and enhance performance. These drugs have been linked to instances of doping in race horses.
This application note describes a Supported Liquid Extraction (SLE) protocol for the extraction of ethylestrenol and stanozolol from horse urine prior to LC-MS/MS analysis.
The method achieves high reproducible analyte recoveries from both gelding and filly urine. Protocols for 48-well, 96-well plate and column formats are described.
This guide describes how to develop supported liquid extraction methods using ISOLUTE® SLE+ products, along with hints and tips for optimizing performance and extending the range of analytes that can be extracted