Part No: AN921Issued year: 2019File size: 1.39mbFile type: pdf
This application note describes the extraction of a panel of 18 steroid hormones from horse hair using ISOLUTE® SLE+. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 80% with RSDs lower than 5% for most analytes. Linearity of greater than 0.999 is achieved for all analytes in the range 0.5-500 pg/mg of hair.
Part No: AN843Issued year: 2015File size: 0.86mbFile type: pdf
Ethylestrenol and stanozolol are anabolic steroids which can be used to increase muscle mass and enhance performance. These drugs have been linked to instances of doping in race horses.
This application note describes a Supported Liquid Extraction (SLE) protocol for the extraction of ethylestrenol and stanozolol from horse urine prior to LC-MS/MS analysis.
The method achieves high reproducible analyte recoveries from both gelding and filly urine. Protocols for 48-well, 96-well plate and column formats are described.