This application note describes the use of ISOLUTE® ENV+ on-line cartridges in a fully automated on-line SPE-LC-MS/MS method for extraction and quantification of 16 acid herbicides in drinking and surface water.

2,4,5-T 2,4-D 2,4-DB Acid herbicides Application Notes Asulam Benazolin Bentazone Bromoxynil Dicamba Dichlorprop Drinking water English Environmental Ioxynil MCPA MCPB Mecoprop On-line SPE On-line SPE-LC-MS/MS Pentachlorophenol Quinmerac Surface water Triclopyr Water

Pesticides, including insecticides, herbicides and fungicides are used extensively to increase agricultural yields. The total world usage of pesticides in 2007 was approximately 5.2 billion pounds with the US consuming approximately 22% of the total.1 The wide use of pesticides yields concern that drinking water sources will become contaminated, exposing the population to hazardous substances that may cause cancer.

Application Notes Disk Drinking water EPA 508.1 Analytes English Environmental GC-MS Pesticides

Analysis of the perfluorinated alkyl acids listed in EPA Method 537 along with fluorotelomeric alcohols and perfluorinated sulfonates were extracted using SPE (using ISOLUTE 101 500 mg/ 6 mL SDVB SPE columns) and analyzed on the LCMS 8045. Minimum Detection Limits(MDL) ranged from 0.69 to 3.25 ppt. Extraction recoveries were greater than 80% for all compounds, with surrogate recoveries within 10% of the true value.

Application Notes Column Drinking water English Environmental LC-MS/MS Polyfluorinated acids

This application note demonstrates the use of automated solid phase extraction for a variety of pharmaceutical compounds in drinking water, wastewater and surface water. The criteria specified in US EPA Method 1694 was met, even at very low concentrations. For lower concentrations a larger sample of water may be processed.

4790 Acetaminophen Albuterol Albuterol Application Notes Atenolol Caffeine Carbamazepine Cotinine DEET Dehydronifedipine Diclofenac Diltiazem Diphenhydramine hydrochloride Disk Drinking water English Environmental Gemfibrozil Ground water Ibuprofen LC-MS TOF Lamotrigine Metoprolol Naproxen Pharmaceuticals Sulfadimethoxine Sulfamethoxazole Triclocarban Trimethoprim Waste water

This application note demonstrates the performance of automated disk-based solid phase extraction (SPE), using Biotage® Horizon 5000 automated extraction system and Atlantic® DVB SPE disks, for concentration of organochlorine pesticides from whole water, prior to GC/MS analysis, following the requirements of EN16693:2015.

1,2 4 trichlorobenzene 1,2,3 trichlorobenzene 1,3,5 trichlorobenzene 5000 Alachlor Aldrin Application Notes DDD DDE DDT Dieldrin Disk Drinking water EN16693 Water Quality EN16693:2015 Analytes Endosulfan Endrin English Environmental GC-MS Hexachlorobenzene Hexachlorobutadiene Isodrin Organochlorine pesticides (OCP) Pentachlorobenzene Water alpha HCH beta HCH delta HCH gamma HCH

Polycyclic aromatic hydrocarbons (PAHs) can be found in a number of water types, such as drinking water, surface water, ground water and wastewater. There are serval types of extraction techniques that can be implemented to remove them from water for measurement including liquid-liquid extraction (LLE), continuous liquid-liquid extraction (CLLE) and solid phase extraction (SPE). With LLE and CLLE there is little selectivity within the methods for difficult matrices or other compound interferences.

5000 Application Notes Disk Drinking water English Environmental GC-MS Ground water PAHs Polyaromatic Hydrocarbons (PAH) Waste water

This study shows that the Biotage® 4790 Automated Extractor system, along with the Atlantic™ DVB disk, the DryDisk® separation membrane, and the DryVap® Concentrator System can be successfully used to analyze for Nonyl Phenol and Bisphenyl A in water samples. This sample preparation process is fully automated, requiring minimal human intervention, and allowing for reductions in sample processing costs.

5000 Application Notes Bisphenol A Disk Drinking water Endocrine disrupter English Environmental Environmental contaminants GC-MS Nonyl phenol Water

EPA Method 525.2 describes the procedure to determine low ppb levels of semi-volatile organic material in drinking water using solid phase extraction (SPE) or liquid-solid extraction (LSE) techniques. The extraction solvents are methylene chloride and ethyl acetate. Extracts are analyzed by GC/MS using a splitless injection technique.

2,4-D 245-T ACIFLUORFEN Application Notes BENTAZON Bentazone Chlorinated acids DCPA, MONO ACID DCPAA-SS DINOSEB Dicamba Disk Drinking water English Environmental GC/ECD PCP PICLORAM SILVEX

Carbaryl, a commonly used insecticide for the past 20 years, has become a significant environmental concern throughout the world. Also known through its trademarked name Sevin, it is a cholinesterase inhibitor and can be toxic to humans, causing problems in the blood, nervous, and reproductive systems.

4790 Application Notes Disk Drinking water English Environmental Insecticides Pesticides

Hormones in drinking water are a growing concern, since the presence of these compounds can potentially be linked to serious health hazards such as human developmental and reproductive side-effects. Hormones are introduced into our environment in several ways. Natural and synthetic hormones that are given to livestock, pass though the animals, runoff into surface water, and leach into underground water supplies. Humans also produce and excrete natural hormone waste every day.

4790 Androgens Clinical Disk Drinking water Endocrine disrupter English Estrogens Hormones

Method 525.2 describes the procedure to determine a full suite of low concentration semi-volatile organic compounds in drinking water using solid phase extraction (SPE) or liquid–solid extraction (LSE) techniques. The City of Fort Worth, Water Department implemented an automated SPE process for the analysis of semi-volatiles by EPA Method 525.2 using the Atlantic® C18 solid phase extraction disk. Ethyl acetate, methanol and water were used to condition the Atlantic C18 disk prior to the extraction step. The extraction solvents used were a 1:1 mixture of methylene chloride and ethyl acetate. Extracts were then analyzed by GC/MS using a splitless injection technique.

Acenaphthylene Alachlor Aldrin Alpha-Chlordane Anthracene Application Notes Atrazine Benzo (a) Anthracene Benzo (b) fluoranthene Benzo (g,h,i) perylene Benzo (k) Fluoranthene Benzo(a)pyrene Bromacil Butylbenzylphthalate Di-(2-ethylhexyl) Phthalate Di-n-butylphthalate Dibenz (a,h) Anthracene Diethyl phthalate Dimethylphosphate (DMP) Disk Drinking water Endrin English Environmental GC-MS Gamma-BHC Gamma-Chlordane Heptachlor Hexachlorobenzene Hexachlorocyclopentadiene Indeno (1,2,3-cd) Pyrene Methoxychlor Metolachor Organic Compounds Pentachlorophenol Phenanthrene Prometon Propachlor Pyrene Simazine Trans-nonachlor Trifluralin

Drinking water is a critical resource important for human health. As water becomes more scarce, quality will become increasingly important and monitoring will be required more frequently and at lower concentration levels. The number of compounds and lower concentrations for monitoring will make extraction and analysis even more challenging.

Application Notes Chinese Environmental Agency Method Chinese Method SL-392-2007 Disk Drinking water English Environmental GC-MS SL-392-2007 SVOC Semi-Volatile Organics

This application note describes the use of the Biotage® Horizon 4790 automated extractor system, and the Atlantic® DVB SPE disk for the extraction of water samples, as specified in EPA method 525.3.

4790 Application Notes Disk Drinking water EPA 525.3 Analytes English Environmental GC-MS US EPA Method 525.3

The second unregulated contaminant monitoring regulation (UCMR2) program was developed to monitor US drinking water sources for currently unregulated compounds. EPA Method 527 is categorized under List 1; Assessment Monitoring in the UCMR2 program. It focuses on a wide range of semi volatile organic contaminants including pesticides that were deferred during the first UCMR, flame retardants, and pyrethroid pesticides. This application for EPA Method 527 employs SPE with analysis by gas chromatography / mass spectrometry (GC/MS).

1,3,dimethyl-2- nitrobenzene Application Notes BDE-100 Dimethoate Disk Drinking water English Environmental GC-MS Hexabromobiphenyl Perylene-d12 Pesticides Terbufos Sulfone Triphenyl phosphate

Drinking water is an important resource that can provide significant exposure to humans if it is polluted. Analysis of source water, which may come from surface water, groundwater or treated water must be evaluated for possible contaminants to ensure regulatory compliance.1 Drinking water at the tap is often evaluated for contaminants that may be introduced through leaks in the pipes or pipe materials.

Application Notes Chinese Environmental Agency Method Disk Drinking water English Environmental GC-MS SL-392-2007 SVOC Semi-Volatile Organics

This application note highlights a SPE disk which was specifically designed to increase the recoveries of traditionally difficult polar compounds as well as help to increase the diversity of the traditional 525.2 list to include those compounds which are of concern in today’s society.

4790 Application Notes Disk Drinking water EPA 525.2 Analytes English Environmental GC-MS Organic Compounds

Method 508.1 is used to determine twenty-nine chlorinated pesticides, three herbicides, and four organohalides in ground water, drinking water, and water in any treatment stage. The analytes are extracted from the water using a 47 mm C18 disk. The disk is extracted on the Horizon Technology SPE-DEX® 4790 Automated Extraction System using Ethyl Acetate and Methylene Chloride (DCM). The extract is then dried and concentrated using the Horizon Technology DryVap® with DryDisk® technology. Final analysis is by GC/ECD.

Application Notes Chorinated Pesticides Drinking water English Environmental GC-MS Ground water Herbicides Organohalides Pesticides US EPA Method 508.1 Water

Method 526 is a gas chromatography / mass spectrometry(GC/MS) method for the determination of selected semivolatile compounds in raw and finished drinking waters.This method efficiently extracts analytes using the SPEDEX® 4790 with an Atlantic® 47 mm polystyrene divinylbenzene (DVB) disk. The disk is extracted with using Ethyl Acetate (EtAc) and Methylene Chloride (DCM). The extract is then dried and concentrated using the DryVap® concentrator system coupled with DryDisk® technology to a final volume of 1.0 mL. Final analysis is done by GC/MS.

Application Notes Disk Drinking water English Environmental GC-MS Semi-Volatile Organics

EPA Method 548.1 details the procedure for the determination of endothall in drinking water by ionexchange solid phase extraction (SPE), acidic methanol methylation, and gas chromatography / mass spectrometry. This note presents data from the State of Idaho Bureau of Labs for the initial demonstration of capability using the Biotage® Horizon T4790 Automated Extractor System.

Application Notes Disk Drinking water EPA 548.1 Analytes Endothall English Environmental GC-MS

Method 549.2 is used to determine diquat and paraquat in drinking water and drinking water sources. The analytes are extracted from the water using a Biotage® Horizon 4790 and 47mm C8 disk.

Application Notes Diquat Disk Drinking water EPA 549.2 Analytes English Environmental Paraquat