Whether you need targeted methods for analytes such as Vitamin D metabolites in serum, or methods suitable for extraction of a wide panel of drugs and metabolites from urine, sample preparation before analysis is essential. This compendium highlights a selection of clinical sample preparation applications from Biotage.

Alcohol metabolite Application Notes Biomarker Brochures Catecholamines Clinical English Pain Management Peptides Product Notes Steroid Hormones Steroid metabolite Thyroid Hormone Tobacco Specific Nitrosamines (TSNAs) Vitamin D Vitamins Water Soluble Vitamins

Traditionally the analysis of aldosterone and angiotensin (for plasma renin activity measurement) are performed separately. However, the relationship of the aldosterone-to-renin-ratio is a very useful tool to help define the cause of secondary hypertension. This poster compares sample preparation options for the simultaneous extraction of Angiotensin I, II and Aldosterone from plasma. ASMS 2018

96-well plate Aldosterone Angiotensin I Angiotensin II, Clinical English LC-MS/MS Peptides Plasma Posters Steroid Hormones

Traditionally the analysis of aldosterone and angiotensin (for plasma renin activity measurement) are performed separately. However, the relationship of the aldosterone-to-renin-ratio is a very useful tool to help define the cause of secondary hypertension. This poster compares sample preparation options for the simultaneous extraction of Angiotensin I, II and Aldosterone from plasma. MSACL EU 2018 MSACL NA 2019

96-well plate Aldosterone Angiotensin I Angiotensin II, Clinical English LC-MS/MS Peptides Posters Steroid Hormones

This application note describes a solid phase extraction (SPE) protocol for the extraction of the peptide desmopressin from human serum prior to LC-MS/MS analysis. The method uses EVOLUTE(R) EXPRESS WCX 96-well plates to achieve high recoveries with minimal matrix effects.

96-well plate Application Notes Clinical Desmopressin English LC-MS/MS Peptides Serum

The method described in this application note achieves high reproducible extraction recoveries of the peptides oxytocin and vasopressin from serum using a fast, simple EVOLUTE® EXPRESS Load-Wash-Elute SPE procedure. Extraction from serum was performed using EVOLUTE EXPRESS ABN 96-well plates, utilizing a highly aqueous elution solvent, and minimizing co-extractable material in the form of proteins, lipids and phospholipids.

96-well plate Application Notes Clinical English LC-MS/MS Oxytocin Peptides Pharma Serum Vasopressin

This application note describes the extraction of several peptides, ranging in size from a 7mer to a 54mer, from plasma and serum using EVOLUTE® ABN in a 96-well plate format. The presence of certain proteins and peptides in human biological matrices can be used as diagnostic markers for the onset of disease and other health problems in humans. The ability to detect and monitor lower levels of peptides can aid in clinical patient health assessments and therapeutic drug level determinations. The sample preparation methodology detailed in this application note can significantly increase sensitivity and detection of peptides from biological matrices on LC/MS/MS. This application note offers the user two different elution strategies due to the extensive combination of peptides that may need to be extracted either strategy may be suitable dependent upon which peptides are relevant. Peptides, EVOLUTE, SPE, Solid Phase Extraction, Proteins,

ACTH (1-24) Alpha-Endorphin Angiotensin Fragment 1-7 Angiotensin II, Application Notes Clinical Elafin Helicobacter pylori (2-20) LC-MS/MS Myelin Glycoprotein fragment (35-55) rat Peptides Plasma Vasoactive Intestinal Fragment 1-12

This application note describes a solid phase extraction (SPE) protocol, using EVOLUTE EXPRESS ABN solid phase extraction plates, for the extraction of teicoplanin from plasma prior to HPLC-DAD analysis.

96-well plate Antibiotics Application Notes Clinical English HPLC-DAD Peptides Teicoplanin

This poster outlines a new method for the extraction of a mixture of peptides from plasma using resin based SPE. Initial method optimization was performed using EVOLUTE ABN before comparison to the streamlined approach without plate conditioning and equilibration using EVOLUTE EXPRESS ABN. Presented at EBF 2012. EBF, EBF 2012, Peptide, ABN, SPE, Polymer, Polymeric,

ACTH (1-24) Alpha-Endorphin Angiotensin Fragment 1-7 Angiotensin II, Elafin Helicobacter pylori (2-20) LC-MS/MS Myelin Glycoprotein fragment (35-55) rat Peptides Pharma Plasma Posters Vasoactive Intestinal Fragment 1-12

This poster describes the use of EVOLUTE® ABN and EVOLUTE® EXPRESS ABN for the fast and efficient extraction of a diverse mixture of peptides spiked into biological matrices. Pittcon 2014

96-well plate ACTH (1-24) Angiotensin Fragment 1-7 Angiotensin II, Clinical Elafin Endorphin English Glycoprotein myelin Helicobacter pylori (2-20) LC-MS/MS Peptides Pharma Plasma Posters Serum Vasoactive Intestinal Fragment 1-12

This poster investigates the selectivity of polymer-based solid phase extraction sorbents and rapid extraction of clinical matrices, matrix interference reduction and improved analyte detection. ASMS 2017

96-well plate Clinical English Oxytocin Peptides Pharma Posters Serum Vasopressin

This application note describes the simultaneous extraction of aldosterone at low (< 25pg/mL) concentration and angiotensin I and II from plasma, with the potential to improve throughput and reduce time and costs in PRA determination.

96-well plate Aldosterone Angiotensin I Angiotensin II, Application Notes Clinical English Extrahera LC-MS/MS Peptides Plasma Steroid Hormones