The method described in this application note achieves high reproducible extraction recoveries of vitamin B7 from serum while minimizing co-extractable material in the form of proteins, lipids and phospholipids. Serum is extracted using the EVOLUTE® EXPRESS ABN 96-well plate.
ISOLUTE® PLD+ Protein and Phospholipid Removal plates offer a substantial improvement in extract cleanliness compared to traditional protein precipitation techniques for bioanalytical sample preparation. This application note describes a simple, effective ISOLUTE® PLD+ protocol for the extraction of 25-hydroxy vitamin D from serum, demonstrating high, reproducible analyte recoveries with low protein and phospholipid content in the extracts. Included in this application note are the detailed settings for implementing the method on the Biotage Extrahera automation system.
This application note describes a protocol for the extraction of 1,25 di-OH Vitamin D2 and 1,25 di-OH Vitamin D3 metabolites from serum using supported liquid extraction prior to LC-MS/MS detection. A calibration range between 5 and 500 pg/mL is demonstrated using a starting volume of 0.25 mL of serum. Sensitivity is maximized through the use of a simple PTAD derivatization and formation of a methylamine complex. The method can be easily automated using the Biotage Extrahera. Details of the automated procedure and data comparing manual and automated method performance are included.
This application note describes the extraction of Vitamin B3 (Niacin, nicotinic acid) and its key metabolites from serum using a strong cation exchange SPE retention mechanism. High reproducible analyte recoveries are achieved.
Vitamin D deficiency can result in a variety of health issues such as osteoporosis, liver and kidney problems, it is often associated with increased risk of cancers and multiple sclerosis. From this standpoint vitamin D monitoring is on the rise and of significant clinical relevance. This method describes the use of ISOLUTE SLE+ plates for the efficient and simple extraction of the vitamin D metabolites 25-OH vitamin D2 and 25-OH vitamin D3. Incorporated into the procedure is an integral protein binding disruption step which maximizes analyte recovery and eliminates the need for any offline protein disruption. This method has been internally validated using DEQAS approved serum samples and deuterated internal standards the results obtained are better than that required for criteria for acceptable performance for DEQAS approval. All recoveries were consistently greater than 90% with all RSDs < 10% at LOQs of 1-3.75 ng/mL. Vitamin, Vitamin D, VitaminD, D, Clinical, SLE+, SLE, ISOLUTE, Metabolites, Supported Liquid Extraction, 25 Hydroxy Vitamin D,
Vitamins A and E have been shown to have antioxidant and anti-inflammatory effects that mammal biological systems use to protect against cell mutation and tissue damage. The extraction of Vitamin A and E from human serum prior to analysis with LC-MS-MS is problematic due to matrix effects. This method outlines a fast, high throughput sample preparation technique that eliminates matrix interferences from serum allowing for mass spectral detection of the target analytes at a concentration of 100 ng/mL or less. Vitamin, A, E, Beta Carotene, Alpha Tocopherol, Retinol, SLE+, SLE, Supported Liquid Extraction, Serum, Clinical, LCMS, Sample Prep