this poster compares different extraction techniques (mixed-mode SPE: ISOLUTE HCX and EVOLUTE EXPRESS CX; Dual mode extraction (ISOLUTE HYDRO DME+) and supported liquid extraction (ISOLUTE SLE+)) for extraction of fentanyl and fentanyl analogues from both urine and whole blood. SOFT 2019
4-ANPP (4-aminophenyl-1-phenethylpiperidine) 4-fluoro-isobutyryl-fentanyl 96-well plate Acryl fentanyl Alfentanil Butyryl fentanyl Carfentanil Clinical English Fentanyl Fentanyls Forensic Furanyl fentanyl Isobutryl fentanyl LC-MS/MS Methoxyacetyl fentanyl Norfentanyl Posters Sufentanil U-47700 U-51754 Urine Valeryl fentanyl Whole blood
The method addresses the extraction of all three classes of drugs of abuse from urine using non-polar and cation exchange retention mechanisms on the ISOLUTE HCX mixed mode column. This protocol was optimized to eliminate the use of dichloromethane in the elution of the acidic / neutral drug fraction.
Acidic drugs Amphetamine Application Notes Basic drugs Benzodiazepines Benzoylecgonine Cocaines Codeine Column Diazepam Forensic Morphine Naphyrone Norcocaine Nordiazepam Opiates Oxazepam THC-COOH Temazepam Urine
This procedure is recommended for the extraction of extensive panel of significant therapeutic and illicit drugs of abuse from whole blood and urine using mixed-mode cation exchange SPE columns. ISOLUTE HCX is a silica based mixed-mode sorbent that combines non-polar and strong cation exchange retention mechanisms for extraction of basic drugs from biological fluids. Ion exchange, Drugs of Abuse, DOA, Clinical,
2-Hydroxyethylflurazepam 4-Hydroxynordiazepam 7-Aminoclonazepam 7-Aminoflunitrazepam 7-Aminonitrazepam Alprazolam Application Notes Benzodiazepines Bromazepam Chlordiazepoxide Clinical Clonazepam Clozapine Demethylflunitrazepam Desalkylflurazepam Diazepam English Estazolam Flunitrazepam Flurazepam Forensic Hydroxyalprazolam Hydroxymidazolam Hydroxytriazolam LC-MS/MS Midazolam Nitrazepam Nordiazepam Oxazepam Prazepam Temazepam Triazolam Urine Whole blood Zolpidem
The following method was developed for the extraction of barbiturate drugs from urine, including: butabarbital, amobarbital, cyclobarbital and phenobarbital. Typical recoveries for these drugs are > 85%. ISOLUTE, BIOTAGE, HCX, DOA, Nervous System
This method was developed for the extraction of phencyclidine from meconium for drugs ofabuse applications. The analyte has an ionisable amine group, which is exploited through cation exchange interactions. The pKa is approximately 8, so the compound is fully ionized at pH values of less than 6. The analyte also has significant non-polar characteristics, and both hydrophobic and ion exchange retention mechanisms are used to give a clean extract. ISOLUTE, PCP, DOA, Varian Bond Elut Certify, UCT CleanScreen DAU
This application was developed for the extraction of phencyclidine and other basic drugs from urine for drugs of abuse applications. Typical recovery for PCP is > 85%. PCP, DOA, Biotage, ISOLUTE, UCT CleanScreen DAU, Varian Bond Elut Certify
ISOLUTE Confirm HCX columns are based on cation exchange and C8 mixed mode chemistries. Basic drugs are therefore retained by two primary retention mechanisms - ionic and non-polar. This allows a more rigorous interference elution regime to be used, leading to a very clean final extract, as many non-polar interferences which are retained by a non-polar interaction alone, can be eluted selectively, prior to elution of the drug.
The extraction of basic drugs from biological fluids using a purely non-polar retention mechanism (e.g. C4, C8 or C18) can lead to extracts that contain a large amount of non-polar co-extracted material that can interfere with subsequent analysis. Conversely, extraction mechanisms based on ion exchange interactions can be non-robust due to the variable ionic strength of the sample matrix.
Sample preparation techniques such as protein precipitation, supported liquid extraction or non-polar SPE may not be selective enough to give extracts of sufficient purity for low level analysis. In these cases, the selective mixed-mode approach to the extraction of basic and acidic drugs is a suitable alternative, giving very high purity extracts with minimal levels of co-extracted material.
This application note describes an automated extraction procedure from Tricyclic Antidepressants from Whole Blood Using ISOLUTE HCX Columns and the RapidTrace Workstation. TCAD, TCA, Tricyclic antidepressants, ISOLUTE,
The typical range of drugs screened using this generic method are Cocaine, D3-Cocaine, Benzoylegonine-TMS, D3-Benzoylegonine-TMS, Amphetamine, D5-Amphetamine, Methamphetamine, Codeine, Morphine, Amitriptyline HCL, Nortriptyline HCL, Imipramine HCL, Desipramine HCL. These drugs represent a panel of the most significantly abused drugs as recommended by The National Institute on Drug Abuse (NIDA). drugs of abuse, drugs testing, amphetamines, BZE, Cocaine, Codeine, Ion exchange,
Amitriptyline Amphetamine Application Notes Benzoylecgonine Biological fluids Butylone Codeine Column Desipramine Forensic Imipramine Methamphetamine Morphine Nortryptiline Plasma RapidTrace Tricyclic Antidepressant Urine