Biotage has developed a range of tools to improve the peptide synthesis workflow, from synthesis of crude peptides through to the final purified product. These solutions make your workflow more efficient and improve the quality of peptides you synthesize. Our workflow solutions are used in pharma, biotech, CRO and academic laboratories throughout the world.
The use of reversed-phase high performance flash chromatography (HPFC) is gaining traction as a strategy to reduce the overall purification time for synthetic peptides. Herein we present data demonstrating that a focused gradient, with a slope reminiscent to that commonly used for HPLC-mediated peptide purification, can indeed be used in HPFC to improve the final purity of a desired peptide with minimal time invested in method development.