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Acidic, Basic, and Neutral Drug Screen of Hydrolyzed Urine

30 March 2016

A new application note developed for clinical and toxicological laboratories describes the extraction of acidic, basic, and neutral drugs from urine for screening purposes using ISOLUTE® SLE+ supported liquid extraction plates prior to LC-MS/MS analysis.


Using a 100 µL sample size, most of the tested analytes were recovered at 70 percent or greater. The analytical response for the extracted analytes with high matrix effects was sufficient to achieve adequate recoveries and LODs. This method, therefore, supports the use of ISOLUTE® SLE+ for the screening of urine samples for various illicit and prescribed drugs.


Codeine, hydrocodone, oxycodone, norcodeine, oxymorphone, 6-acetyl codeine, alprazolam, clonazepam, diazepam, flunitrazepam, nitrazepam, oxazepam, temazepam, dextromethorphan, buprenorphine, norbuprenorphine, fentanyl, EDDP, benzoylecgonine, tetrahydrocannabinol, normeperidine, naltrexone, hydromorphone, propoxyphene, pentazocine, amphetamine, norfentanyl, MDEA, butalbital, pentobarbital, phenobarbital, secobarbital, 11-nor-9 –carboxy- Δ9-tetrahydrocannabinol, and d3-11-hydroxy-tetrahydrocannabinol.

Download the application note below. 

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ISOLUTE® SLE+ Supported Liquid Extraction