This application note describes the extraction of the immunosuppressant drug mycophenolic acid and its metabolite mycophenolic acid glucuronide from fortified serum using ISOLUTE® SLE+ in a 96-well plate format.
The ability to quantitate the amount of free drug and metabolite in patient serum is supported by using a fast and efficient supported liquid extraction sample preparation method using ISOLUTE® SLE+plates. The free drug and metabolite can be recovered from serum using ISOLUTE SLE+ with high enough efficiency to allow for quantitation at target trough levels.
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