Biotage labs have released an application note describing the extraction of cocaine and its metabolites from spiked oral fluid collected with the Quantisal Oral Collection device prior to GC-MS.

This application note details effective protocols for optimized for loading volumes of either 400 μL or 1 mL. The simple sample preparation procedure delivers clean extracts and analyte recoveries ranging from 85–99% with RSDs below 10% for all analytes.

ISOLUTE® SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation, and significantly reduced sample preparation.

Analytes

Cocaine, AEME, EME, Benzoylecgonine, Cocaethylene and Norcocaine.

Typical analyte % recoveries for extracted cocaine and metabolites from urine (n=7) using the described ISOLUTE® SLE+ protocol.

More Application Notes from Biotage »