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Extraction of cocaine using SLE+ banner

Extracting Cocaine from Oral Fluid

07 November 2014

Biotage labs have released an application note describing the extraction of cocaine and its metabolites from spiked oral fluid collected with the Quantisal Oral Collection device prior to GC-MS.


Structures of cocaine and its metabolitesThis application note details effective protocols for optimized for loading volumes of either 400 μL or 1 mL. The simple sample preparation procedure delivers clean extracts and analyte recoveries ranging from 85–99% with RSDs below 10% for all analytes.

ISOLUTE® SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation, and significantly reduced sample preparation.


Cocaine, AEME, EME, Benzoylecgonine, Cocaethylene and Norcocaine.

Typical analyte % recoveries for extracted cocaine and metabolites from urine (n=7) using the described ISOLUTE® SLE+ protocol.

More Application Notes from Biotage »

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