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Extraction of Barbiturates from Oral Fluid Using ISOLUTE® SLE+

31 January 2017

This application note describes the extraction of barbiturates from oral fluid matrix collected using the NeoSal™ collection devices, prior to GC/MS analysis.


Barbiturates are central nervous system depressants with sedatives properties in medicine. As with many other drugs they also also have recreational use with high addiction rates and risk of overdosing.

This application note describes an effective and efficient ISOLUTE SLE+ protocol optimized for 1 mL sample volumes. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 88% with RSDs lower than 6% for all analytes.

ISOLUTE® SLE+ supported liquid extraction plates and columns offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation, and significantly reduced sample preparation.