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Extraction of Benzodiazepines From Oral Fluid Using ISOLUTE® SLE+

15 February 2017

This application note describes the extraction of benzodiazepine compounds from oral fluid matrix collected with the NeoSal™ devices, prior to GC/MS analysis.


The protocol described is optimized for the 1 mL capacity ISOLUTE SLE+ column format. The simple sample preparation procedure delivers clean extracts and analyte recoveries greater than 70% with RSDs lower than 10% for all analytes.

ISOLUTE® SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation, and significantly reduced sample preparation.

Download the application note below. 


  • Diazepam
  • Nordiazepam
  • Midazolam
  • 7-amino-flunitrazepam
  • Bromazepam
  • Oxazepam
  • Nitrazepam
  • Flurazepam
  • Temazepam
  • Lorazepam
  • Clonazepam
  • 2- hydroxy-ethylflurazepam
  • Alprazolam
  • Triazolam
  • Alpha-hydroxyalprazolam
  • Alpha-hydroxytriazolam

This concludes the Biotage Application Note series covering extraction of all the major drug classes from NeoSal oral fluid collection devices.

Find related application notes here »

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ISOLUTE® SLE+ Supported Liquid Extraction