Phosphatidylethanol (PEth) is an alcohol biomarker with a high degree of specificity; blood concentration of PEth correlates to the amount of alcohol consumed. This application note describes the extraction of three common species of PEth from whole blood using ISOLUTE® SLE+ supported liquid extraction prior to HPLC-MS/MS analysis. This simple sample preparation procedure produces clean extracts, good recoveries with low RSD, and LOQ from 20 ng/mL, and can be fully automated using Biotage® Extrahera™. 96 samples can be processed in 22 minutes and 33 seconds.
ISOLUTE SLE+ supported liquid extraction fixed-well plates offer an efficient alternative to traditional liquid-liquid extraction (LLE) for bioanalytical sample preparation, providing high analyte recoveries, no emulsion formation, and significantly reduced sample preparation time.
Two New Methods for Extraction of a Steroid Hormone Panel from Human Urine
New Method for Extraction of a Steroid Panel from Horse Hair
New Method for Analysis of Environmental Pesticides in Water
"I Was Surprised that Everything Worked So Well". A Researcher on Moving to Automated Sample Prep
Notice of Discontinuation of Biotage® SNAP Flash Columns