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High Recovery And Yield Of Amphipathic Peptide ‘18A’

03 November 2015

This application note highlights the excellent quality of synthesis that was achieved using the Biotage® Initiator+ Alstra™ microwave peptide synthesizer and after purification by RP-HPLC, a large quantity of pure peptide was recovered (>450 mg) with a high yield of 52%.


Structural and functional studies of membrane proteins remain a challenge due to difficulties in their handling. The combination of using high concentrations of reagents (0.5M) and fast and precise microwave heating to increase the coupling efficiency using the Biotage® Initiator+ Alstra™ microwave peptide synthesizer, enabled the efficient synthesis of peptide ‘18A’ and simplified the subsequent purification by RP-HPLC despite its relatively low solubility, to afford the desired peptide in 52% yield (468 mg). Read the full application note using the link below.


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High recovery and yield of amphipathic peptide 18A

Part No: AN103 Published: 2015 0.25 MB Format: pdf

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