The research groups of Prof. Blixt and Prof. Jensen at the University of Copenhagen have described a high-throughput O-glycopeptide discovery platform for seromic profiling, using the Syro II parallel peptide synthesizer for high-throughput glycopeptide synthesis where over 1700 O-glycopeptides were synthesized for the construction of novel peptide microarrays.This approach provided multiwell low scale synthesis of peptides and GalNAc-glycopeptides combined with direct printing without prior purification for a facile and economical approach to display hundreds of glycopeptides. Biomarker microarrays are becoming valuable tools for serological screening of disease-associated autoantibodies.
The research for this application note was carried out in the groups of Prof. Ola Blixt and Prof. Knud J. Jensen at the University of Copenhagen.
Example of 20-mer O-glycopeptide Tn-Muc1a.
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