"A question I hear a lot from chemists is “how much can I load”. The answer is always “it depends on your separation quality”. At that point I begin asking about the TLC data and purification goals. Purification goal setting should be your first step and the question to answer is – what do I need this purification to achieve? Is the goal high purity, high yield, or some combination. Remember, you will typically sacrifice purity for high yield and yield for high purity so optimization is an important consideration.
In this post I will discuss the results of a study I conducted where I continually increased the crude sample load until my target compound purity fell below specific targeted levels to understand the real loading limitation."
Read the full story on the Flash Purification Blog »
Yes, You Can Add Biotage® Sfär Columns to Your Isolera™ System
Two New Applications Notes - Sample Preparation of a Drugs of Abuse Panel in Human Hair
Biotage Acquires Protein Purification Company PhyNexus Inc
Why We Selected ISOLUTE® SLE+ for Luteolin Extraction
Application Video AN882 Extraction of Oxytocin and Vasopressin from Serum