ISOLUTE® NH2 solid phase extraction cartridges work well for detecting alcohol biomarkers in human urine, and a recent study reported a successful throughput of 6,000 samples per year. The key advantage to this method is the elimination of the evaporation step prior to analysis by LC-MS/MS.
A new application note from Biotage (AN818.v1) offers some suggestions to optimize this method further, and also verifies the method on patient samples. The ISOLUTE NH2 cartridge format demonstrated as a viable option for urine measurements over a relevant concentration range in clinical reference laboratories.
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