Catecholamines and metanephrines are biomarkers used for the detection of diseases such as hypertension, pheochromocytoma and neuroblastoma. The presented poster describes an EVOLUTE® EXPRESS WCX method for simultaneous extraction and analysis of these important clinical analytes. The method uses a very low elution volume from the 10 mg format plates, eliminates the need for any evaporation steps, and is easily automated using Biotage® Extrahera™ sample processor.
EVOLUTE® EXPRESS combines sorbent wettability with optimized SPE components, allowing better flow consistency and in many cases eliminating the need for SPE column conditioning. Therefore the method was replicated without phase conditioning.
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