Just like the North Star has guided sailors and astronomers for centuries, Isolera™ Assist guides you in an intelligent way to select optimal cartridges based on crude sample mass and TLC data.
Using Isolera Assist is like having a senior chemist/mentor with 30 years of experience watching over your shoulder. Helping you to produce successful purifications with a minimum of risk, time spent and cost.
Isolera™ Assist is installed on all new Isolera systems, just go to the product page and ask for a quote.
If you already own an Isolera system, contact your Biotage representative or use any of the following numbers:
Isolera Assist offers two experience levels – for confident users, the advanced user profile opens up all of the functionality of the Isolera system. Alternatively, users can opt for a basic profile, where a streamlined interface ensures the user is up and running in the minimum time. User levels also assist in optimizing open access environments, where the two user levels operate side by side on the system.
Like the Isolera just as it is? If you are already very familiar with the Isolera, just use the ‘Chemistry’ menu button to use the classic Isolera interface.
You just have to follow the orange colored trail and you will be guided through to a successful purification.
First select which user you are. Each user can have specific methods tied to their account, depending on their way of running flash chromatography.
A list of methods for the selected user is displayed. Select the method you want to run. Enter the quantity of you sample. This way the instrument can propose the fastest and most cost effective cartridge for your separation. To optimize the run even further, you may enter TLC data for your sample. If you do not have any TLC data, just press Create to go to the next step.
Enter the solvent strength for your TLC run. Now hold your TLC plate next to the screen and adjust the solvent front and spots to each position from the TLC plate. The Rf values are automatically calculated. No need to use a ruler ever again. If you add another TLC plate with different solvent strength, you can create a step-gradient to save even more time and solvent. Press Create when you are done.
If you add a second TLC you will get a Step Gradient.
The fastest and most cost effective cartridge is selected. You can view solvent consumption and time for the run. If you want to run a different cartridge, select it from the list. Green indicates that the sample load you entered is suitable for the cartridge.
The proposed ideal gradient is generated and you are ready to run. You can change the gradient if you wish.
If you select a previous run, you can optimize the gradient even further.
Click on the peak of interest to generate a shorter optimized step gradient. This ensures an even faster separation with minimal amount of solvent consumption.
Isolera™ Assist brings you the benefits of the feature-rich Isolera Flash Purification Systems in a simple and easy to use interface. It ensures you achieve the best results, regardless of your experience level. Ask for a quote on a system now at the product page, or contact your Biotage representative.
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