The objective of this study was to determine feasibility of transferring a validated liquid-liquid extraction method to a supported liquid extraction method using Biotage ISOLUTE® SLE+ 96-well plates (400μL sample capacity). The laboratory supporting this study processes ~550 samples per month supporting vitamin D analysis in plasma and serum (mixed samples/not separated). During this evaluation, it was determined that sample processing time was reduced by ~50% for a de-identified patient set (n=30, split and processed by both methods). Towards high throughput analysis platforms, ISOLUTE SLE+ was demonstrated as a viable solution in the sample preparation workflow for vitamin D applications.
This study was performed In conjunction with collaborators at the Seattle Children’s Hospital.
Two New Methods for Extraction of a Steroid Hormone Panel from Human Urine
New Method for Extraction of a Steroid Panel from Horse Hair
New Method for Analysis of Environmental Pesticides in Water
"I Was Surprised that Everything Worked So Well". A Researcher on Moving to Automated Sample Prep
Notice of Discontinuation of Biotage® SNAP Flash Columns