This application note describes the extraction of Vitamin B3 (Niacin, nicotinic acid) and its key metabolites from serum using a strong cation exchange SPE retention mechanism. High reproducible analyte recoveries are achieved.
Tags: 96-well plate, Application Notes, B Vitamins, Clinical, English, LC-MS/MS, Niacin (nicotinic acid), Niacinamide, Nicotinuric acid, Serum, Vitamin B3, Vitamins
The first decision that needs to be made is what the final analysis will be for the analyte. This will have an impact on the sample and cartridge size, as well as the final elution solvent. Gas chromatography offers higher sensitivity than HPLC, while HPLC is better suited for ionisable species and very high molecular weights. If LC-MS is available, minimal sample clean-up may be required.
Tags: Manuals & User Guides
This poster describes a strong cation exchange SPE protocol for extraction of Vitamin B3 (Niacin) and related metabolites from serum.
The ISOLUTE SCX-3 96 well plate format demonstrated as a viable option for serum measurements over a relevant concentration range in clinical diagnostics.
It is anticipated that strategies presented in this poster would be of broad-based interest to clinical laboratories focused on combining parent/metabolite assays into a single analysis.
Tags: 96-well plate, B Vitamins, Clinical, English, LC-MS/MS, Niacin (nicotinic acid), Niacinamide, Nicotinuric acid, Posters, Serum, Vitamin B3, Vitamins
The ISOLUTE® family of cation exchange sorbents are used to extract organic cations (basic compounds capable of exhibiting a positive charge) from both aqueous and non-aqueous matrixes. Although extraction is by the same mechanism, each sorbent has properties that influence the way they are used.
Tags: English, Product Notes
ISOLUTE® Si-TsOH (SCX-3) is the bound equivalent of p-toluene sulfonic acid with a
pKa <1. Similar to ISOLUTE propylsulfonic acid (SCX-2)1 , it can be used to (a) scavenge
amines and other bases such as anilines and borohydrides (b) as an acid catalyst in reactions
or (c) as a replacement for aqueous or organic acids in quenching reactions.
Tags: Product Notes
Biotage presented five new posters at the ASMS conference in San Diego this June. We've collected them all below for download. P177: Comparison of Sample Preparation Options for the Extr...11 January 2018
The Biotage 2018 catalog of analytical sample preparation products for chemistry professionals is now available. This edition includes a comprehensive range of sample preparation consumables for bi...17 July 2017
On July 1st, Biotage expanded its direct sales organization and opened the door to analytical customers in Italy. Account Manager Gianluca Pessoni is added to the Italian team, filling the ana...28 June 2017
This application note from Biotage Labs describes a method for extracting nitrosamines from urine. The ISOLUTE® SLE+ sample preparation method allows for a fast extraction with sufficient sampl...15 June 2017
This application note describes the extraction of amphetamine and amphetamine-style compounds from hair matrix prior to GC/MS analysis following bead homogenization. This application note describe...20 March 2017
This application note describes the operating conditions for the automated fractionation of EPH into aliphatic and PAH fractions using the ISOLUTE® EPH column in conjunction with the Biotage&re...20 February 2017
This application note describes the extraction of 49 drugs of abuse from whole blood, prior to UPLC-MS/MS analysis. An effective and efficient ISOLUTE SLE+ protocol optimized the for ...05 January 2016
Now available, the Biotage 2016 catalog of analytical sample preparation products for chemistry professionals. This edition includes a comprehensive range of products for bioanalysis, forensic, cli...11 August 2015
Dilute and shoot (D&S) methods are promoted for use by analytical instrument vendors in clinical, forensic toxicology and bioanalytical laboratories. More sensitive LC-MS/MS systems make this s...27 March 2015
Biotage, pioneers in flash chromatography, started packing columns with silica for flash purification 30 years ago. Some of the first employees are still with us today. In this video, site director...08 January 2015
Now available, the Biotage 2015 catalog of analytical sample preparation products for chemistry professionals. This edition includes a comprehensive range of products for bioanalysis, forensic, cli...29 July 2014
Buy your Biotage consumables through our website and shipping is free For a limited time, free shipping is applied to Biotage consumables orders through the Biotage website*. Let Biotage make orde...16 April 2014
Four new application notes for the sample preparation laboratory from Biotage’s applications development program. AN809 Extraction of THC and Metabolites Including 11-nor-9-c...
What is the maximum capacity of an SPE column?
The capacity of a sorbent is defined as the total mass of strongly retained analyte that can be retained by a given mass of the sorbent under optimum conditions.
For most sorbents, this can range from 1-5% by weight. 100 mg of sorbent might retain 1-5 mg of analyte.
Ion exchange sorbents typically have a capacity of 0.3-1 meq/g.Browse more questions and answers >
|Part No.||Description||Product type||Pack Size||Price|
||533-0002-A||ISOLUTE® SCX-3 25 mg/1 mL||Consumables||100||Log in for price|
||533-0005-A||ISOLUTE® SCX-3 50 mg/1 mL||Consumables||100||Log in for price|
|533-0010-A||ISOLUTE® SCX-3 100 mg/1 mL||Consumables||100||Log in for price|
||533-0010-B||ISOLUTE® SCX-3 100 mg/3 mL||Consumables||50||Log in for price|
||533-0020-B||ISOLUTE® SCX-3 200 mg/3 mL||Consumables||50||Log in for price|
||533-0025-P01||ISOLUTE®-96 SCX-3 25 mg Fixed Well Plate||Consumables||1||Log in for price|
|533-0050-B||ISOLUTE® SCX-3 500 mg/3 mL||Consumables||50||Log in for price|
||533-0050-C||ISOLUTE® SCX-3 500 mg/6 mL||Consumables||30||Log in for price|
||533-0050-P01||ISOLUTE®-96 SCX-3 50 mg Fixed Well Plate||Consumables||1||Log in for price|
||533-0100-B||ISOLUTE® SCX-3 1 g/3 mL||Consumables||50||Log in for price|
||533-0100-C||ISOLUTE® SCX-3 1 g/6 mL||Consumables||30||Log in for price|
||533-0100-P01||ISOLUTE®-96 SCX-3 100 mg Fixed Well Plate||Consumables||1||Log in for price|
||533-0200-CG||ISOLUTE® SCX-3 2 g/6 mL (Tabless)||Consumables||30||Log in for price|
||533-0200-D||ISOLUTE® SCX-3 2 g/15 mL||Consumables||20||Log in for price|
||533-0500-E||ISOLUTE® SCX-3 5 g/25 mL||Consumables||20||Log in for price|
||533-1000-F||ISOLUTE® SCX-3 10 g/70 mL||Consumables||16||Log in for price|